Serratia infection laboratory findings

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Culture Growth

According to the Centers for Disease Control and Prevention (CDC), the following procedure should be followed to properly culture "Serratia:

• Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)
• Growth Conditions:

• Temperature: 35 °C (95 °F)
• Atmosphere: Aerobic

• Procedure

• Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)
• Open vial aseptically to avoid contamination
• Using a sterile loop, remove a small amount of frozen isolate from the top of the vial
• Aseptically transfer the loop to BAP
• Use streak plate method to isolate single colonies
• Incubate inverted phase at 35 °C (95 °F) for 18-24 hours

MIC Results and Interpretation

The following table demonstrates the MIC results following Serratia culture/antibiogram and interpretation

MIC (µg/ml) Results and Interpretation	'	'
Drug	MIC	Interpretation
Amikacin	2	S
Amoxicillin/Clavulanic Acid *	> 32	R
Ampicillin	> 32	R
Ampicillin/Sulbactam	> 32	R
Aztreonam	16	R
Cefazolin	> 32	R
Cefepime	≤ 0.5	S
Cefotaxime	≤ 1	S
Cefoxitin	2	S
Ceftazidime	1	S
Ceftriaxone	≤ 1	S
Ciprofloxacin	≤ 0.12	S
Colistin	> 4	_
Doripenem	_	_
Ertapenem	> 16	R
Gentamicin	0.5	S
Imipenem	> 32	R
Imipenem + chelators **	_	_
Levofloxacin	≤ 0.25	S
Meropenem	> 16	R
Piperacillin/Tazobactam *	2	S
Polymyxin B	> 4	_
Tetracycline	8	I
Tigecycline ***	1	S
Tobramycin	1	S
Trimethoprim/Sulfamethoxazole (TMP - SMX)	2	S