Serratia infection laboratory findings
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Overview
Laboratory Findings
Culture Growth
According to the Centers for Disease Control and Prevention (CDC), the following procedure should be followed to properly culture "Serratia:[1]
- Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)
- Growth Conditions:
- Temperature: 35 °C (95 °F)
- Atmosphere: Aerobic
- Procedure
- Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)
- Open vial aseptically to avoid contamination
- Using a sterile loop, remove a small amount of frozen isolate from the top of the vial
- Aseptically transfer the loop to BAP
- Use streak plate method to isolate single colonies
- Incubate inverted phase at 35 °C (95 °F) for 18-24 hours
MIC Results and Interpretation
The following table demonstrates the MIC results following Serratia culture/antibiogram and interpretation:
| MIC (µg/ml) Results and Interpretation | ||||||||
| Drug | MIC | Interpretation | ||||||
| Amikacin | 2 | S | ||||||
| Amoxicillin/Clavulanic Acid * | > 32 | R | ||||||
| Ampicillin | > 32 | R | ||||||
| Ampicillin/Sulbactam | > 32 | R | ||||||
| Aztreonam | 16 | R | ||||||
| Cefazolin | > 32 | R | ||||||
| Cefepime | ≤ 0.5 | S | ||||||
| Cefotaxime | ≤ 1 | S | ||||||
| Cefoxitin | 2 | S | ||||||
| Ceftazidime | 1 | S | ||||||
| Ceftriaxone | ≤ 1 | S | ||||||
| Ciprofloxacin | ≤ 0.12 | S | ||||||
| Colistin | > 4 | _ | ||||||
| Doripenem | _ | _ | ||||||
| Ertapenem | > 16 | R | ||||||
| Gentamicin | 0.5 | S | ||||||
| Imipenem | > 32 | R | ||||||
| Imipenem + chelators ** | _ | _ | ||||||
| Levofloxacin | ≤ 0.25 | S | ||||||
| Meropenem | > 16 | R | ||||||
| Piperacillin/Tazobactam * | 2 | S | ||||||
| Polymyxin B | > 4 | _ | ||||||
| Tetracycline | 8 | I | ||||||
| Tigecycline *** | 1 | S | ||||||
| Tobramycin | 1 | S | ||||||
| Trimethoprim/Sulfamethoxazole (TMP - SMX) | 2 | S | ||||||
References
- ↑ Antimicrobial Resistance Bank - Serratia marcescens. Centers for Disease Control and Prevention. http://www.cdc.gov/drugresistance/pdf/0122.pdf. Accessed on January 5 2016