The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms.[3]
Clinical significance
AKAP4 is a potential biomarker for early diagnosis and immunotherapy of colon cancer.[4] AKAP4 may be implicated as a biomarker and immunotherapeutic target for cervical cancer.[5] AKAP4 is also a circulating biomarker for non-small cell lung cancer.[6]
↑Turner RM, Johnson LR, Haig-Ladewig L, Gerton GL, Moss SB (November 1998). "An X-linked gene encodes a major human sperm fibrous sheath protein, hAKAP82. Genomic organization, protein kinase A-RII binding, and distribution of the precursor in the sperm tail". The Journal of Biological Chemistry. 273 (48): 32135–41. doi:10.1074/jbc.273.48.32135. PMID9822690.
↑Mohapatra B, Verma S, Shankar S, Suri A (March 1998). "Molecular cloning of human testis mRNA specifically expressed in haploid germ cells, having structural homology with the A-kinase anchoring proteins". Biochemical and Biophysical Research Communications. 244 (2): 540–5. doi:10.1006/bbrc.1998.8079. PMID9514854.
↑Agarwal, Sumit; Saini, Shikha; Parashar, Deepak; Verma, Archana; Jagadish, Nirmala; Batra, Aruna; Suri, Sushma; Bhatnagar, Amar; Gupta, Anju (2013-05-01). "Expression and humoral response of A-kinase anchor protein 4 in cervical cancer". International Journal of Gynecological Cancer: Official Journal of the International Gynecological Cancer Society. 23 (4): 650–658. doi:10.1097/IGC.0b013e31828a0698. ISSN1525-1438. PMID23478221.
↑ 7.07.1Brown PR, Miki K, Harper DB, Eddy EM (June 2003). "A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum". Biology of Reproduction. 68 (6): 2241–8. doi:10.1095/biolreprod.102.013466. PMID12606363.
↑Miki K, Eddy EM (December 1998). "Identification of tethering domains for protein kinase A type Ialpha regulatory subunits on sperm fibrous sheath protein FSC1". The Journal of Biological Chemistry. 273 (51): 34384–90. doi:10.1074/jbc.273.51.34384. PMID9852104.
Further reading
Lester LB, Scott JD (1997). "Anchoring and scaffold proteins for kinases and phosphatases". Recent Progress in Hormone Research. 52: 409–29, discussion 429–30. PMID9238861.
Miki K, Eddy EM (December 1998). "Identification of tethering domains for protein kinase A type Ialpha regulatory subunits on sperm fibrous sheath protein FSC1". The Journal of Biological Chemistry. 273 (51): 34384–90. doi:10.1074/jbc.273.51.34384. PMID9852104.
Turner RM, Foster JA, Gerton GL, Moss SB, Patrizio P (August 2001). "Molecular evaluation of two major human sperm fibrous sheath proteins, pro-hAKAP82 and hAKAP82, in stump tail sperm". Fertility and Sterility. 76 (2): 267–74. doi:10.1016/S0015-0282(01)01922-7. PMID11476771.
Ficarro S, Chertihin O, Westbrook VA, White F, Jayes F, Kalab P, Marto JA, Shabanowitz J, Herr JC, Hunt DF, Visconti PE (March 2003). "Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation". The Journal of Biological Chemistry. 278 (13): 11579–89. doi:10.1074/jbc.M202325200. PMID12509440.
Brown PR, Miki K, Harper DB, Eddy EM (June 2003). "A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum". Biology of Reproduction. 68 (6): 2241–8. doi:10.1095/biolreprod.102.013466. PMID12606363.
Moretti E, Scapigliati G, Pascarelli NA, Baccetti B, Collodel G (September 2007). "Localization of AKAP4 and tubulin proteins in sperm with reduced motility". Asian Journal of Andrology. 9 (5): 641–9. doi:10.1111/J.1745-7262.2007.00267.X. PMID17712481.
