Double-strand-break repair protein rad21 homolog is a protein that in humans is encoded by the RAD21gene.[1][2]
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.[2]
↑McKay MJ, Troelstra C, van der Spek P, Kanaar R, Smit B, Hagemeijer A, Bootsma D, Hoeijmakers JH (Jan 1997). "Sequence conservation of the rad21 Schizosaccharomyces pombe DNA double-strand break repair gene in human and mouse". Genomics. 36 (2): 305–15. doi:10.1006/geno.1996.0466. PMID8812457.
↑Hakimi, Mohamed-Ali; Bochar Daniel A; Schmiesing John A; Dong Yuanshu; Barak Orr G; Speicher David W; Yokomori Kyoko; Shiekhattar Ramin (Aug 2002). "A chromatin remodelling complex that loads cohesin onto human chromosomes". Nature. England. 418 (6901): 994–8. Bibcode:2002Natur.418..994H. doi:10.1038/nature01024. ISSN0028-0836. PMID12198550.
Further reading
Nomura N, Nagase T, Miyajima N, et al. (1995). "Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 1 (5): 223–9. doi:10.1093/dnares/1.5.223. PMID7584044.
Sadano H, Sugimoto H, Sakai F, et al. (2000). "NXP-1, a human protein related to Rad21/Scc1/Mcd1, is a component of the nuclear matrix". Biochem. Biophys. Res. Commun. 267 (1): 418–22. doi:10.1006/bbrc.1999.1969. PMID10623634.
Hoque MT, Ishikawa F (2001). "Human chromatid cohesin component hRad21 is phosphorylated in M phase and associated with metaphase centromeres". J. Biol. Chem. 276 (7): 5059–67. doi:10.1074/jbc.M007809200. PMID11073952.
Sook Kim M, Hyen Baek J, Bae MK, Kim KW (2001). "Human rad21 gene, hHR21(SP), is downregulated by hypoxia in human tumor cells". Biochem. Biophys. Res. Commun. 281 (5): 1106–12. doi:10.1006/bbrc.2001.4488. PMID11243849.
Severin DM, Leong T, Cassidy B, et al. (2001). "Novel DNA sequence variants in the hHR21 DNA repair gene in radiosensitive cancer patients". Int. J. Radiat. Oncol. Biol. Phys. 50 (5): 1323–31. doi:10.1016/S0360-3016(01)01608-X. PMID11483345.
Gregson HC, Schmiesing JA, Kim JS, et al. (2002). "A potential role for human cohesin in mitotic spindle aster assembly". J. Biol. Chem. 276 (50): 47575–82. doi:10.1074/jbc.M103364200. PMID11590136.
Deng Y, Li S, Li X, Magali T (2002). "Expression of hHR21sp gene by peripheral blood and hematopoietic cells of normal subjects and Fanconi anemia patients". Chin. Med. J. 114 (12): 1295–9. PMID11793857.
Chen F, Kamradt M, Mulcahy M, et al. (2002). "Caspase proteolysis of the cohesin component RAD21 promotes apoptosis". J. Biol. Chem. 277 (19): 16775–81. doi:10.1074/jbc.M201322200. PMID11875078.
Porkka KP, Tammela TL, Vessella RL, Visakorpi T (2004). "RAD21 and KIAA0196 at 8q24 are amplified and overexpressed in prostate cancer". Genes Chromosomes Cancer. 39 (1): 1–10. doi:10.1002/gcc.10289. PMID14603436.