Citrus canker

Xanthomonas axonopodis
Scientific classification
Kingdom: Bacteria Phylum: Proteobacteria Class: Gamma Proteobacteria Order: Xanthomonadales Family: Xanthomonadaceae Genus: Xanthomonas Species: X. axonopodis
Binomial name
Xanthomonas axonopodis (Hasse, 1915)
Type strain
ATCC 19312
Synonyms
Pseudomonas citrii Xanthomonas citri

Citrus canker is a disease affecting citrus species that is caused by the bacterium Xanthomonas axonopodis. Infection causes lesions on the leaves, stems, and fruit of citrus trees, including lime, oranges, and grapefruit. While not harmful to humans, canker significantly affects the vitality of citrus trees, causing leaves and fruit to drop prematurely; a fruit infected with canker is safe to eat but too unsightly to be sold.

The disease, which is believed to have originated in South East Asia, is extremely persistent when it becomes established in an area, making it necessary for all citrus orchards to be destroyed for successful eradication of the disease. Australia, Brazil and the United States are currently suffering from canker outbreaks.

Biology

Xanthomonas axonopodis is a rod shaped gram negative bacterium with polar flagella. The bacterium has a genome length approximately 5 Mbp. There are a number of types of citrus canker disease caused by different pathovars and variants of the bacterium:

• The Asiatic type of canker (Canker A), X. axonopodis pv. citri, caused by a group of strains originally found in Asia, is the most widespread and severe form of the disease.
• Cancrosis B, caused by a group of X. axonopodis pv. aurantifolii strains originally found in South America is a disease of lemons, key lime, bitter orange, and pomelo.
• Cancrosis C, also caused by strains within X. axonopodis pv. aurantifolii, only infects key lime and bitter orange.
• A* strains, discovered in Oman, Saudi Arabia, Iran, and India, only infects key lime.

Pathology

File:Citrus canker on foliage.jpg

Lesions on leaves

Plants infected with citrus canker have characteristic lesions on leaves, stems, and fruit with raised, brown, water-soaked margins, usually with a yellow halo or ring effect around the lesion. Older lesions have a corky appearance, still in many cases retaining the halo effect. The bacterium propagates in lesions in leaves, stems, and fruit. The lesions ooze bacterial cells that, when dispersed by wind and rain, can spread to other plants in the area. Infection may spread further by large storm events including tornadoes and hurricanes. The disease can also be spread by using contaminated equipment on or near infected trees, by workers who have come in contact with infected trees, and by transport of infected or contaminated plants or fruit.

Citrus canker bacteria can enter through a plant's stomata or through wounds on leaves or other green parts. In most cases, younger leaves are considered to be the most susceptible. Also, damage caused by Citrus Leaf Miner larvae (Phyllocnistis citrella) can be sites for infection to occur. Symptoms appear from 14 to 60 or more days after infection. Citrus canker bacteria can stay viable in old lesions and other plant surfaces for several months.

File:Citrus canker on fruit.jpg

Citrus canker lesions on fruit.

Detection

The disease can be detected in orchards and on fruit by the appearance of lesions. Early detection is critical in quarantine situations. Bacteria are tested for pathogenicity by inoculating multiple citrus species with the bacterium. Simultaneously, other diagnostic tests (antibody detection, fatty-acid profiling, and genetic procedures using PCR) are conducted to identify the particular canker strain.

Susceptibility

Most varieties of citrus are susceptible to citrus canker. Some species are more susceptible than others, while a few species are resistant to infection.

Susceptibility	Variety
Highly susceptible	Grapefruit (Citrus x paradisi), Key lime (C. aurantiifolia), Pointed leaf Hystrix (C. hystrix), lemon (C. limon)
Susceptible	Limes (C. latifolia) including Tahiti lime, Palestine sweet lime; Trifoliate orange (Poncirus trifoliata); Citranges/Citrumelos (P. trifoliata hybrids); Tangerines, Tangors, Tangelos (C. reticulata hybrids); Sweet oranges (C. sinensis); Bitter oranges (C. aurantium)
Resistant	Citron (C. medica), Mandarins (C. reticulata)
Highly resistant	Calamondin (X Citrofortunella), Kumquat (Fortunella spp.)
Modified from: Gottwald, T.R. et al (2002). Citrus canker: The pathogen and its impact. Online. Plant Health Progress

Management

Citrus canker outbreaks are prevented and managed in a number of ways. In countries that do not have canker, the disease is prevented from entering the country by quarantine measures. In countries with new outbreaks, eradication programs that are started soon after the disease has been discovered have been successful; such programs rely on destruction of affected orchards. When eradication has been unsuccessful and the disease has become established, management options include replacing susceptible citrus cultivars with resistant cultivars, applying preventive sprays of copper-based bactericides, and destroying infected trees and all surrounding trees within an appropriate radius.

Distribution and economic impact

Citrus canker is thought to originate in the area of Southeast Asia-India. It is now also present in Japan, South and Central Africa, the Middle East, the Pacific Islands, some countries in South America, and Florida. Some areas of the world have eradicated citrus canker and others have ongoing eradication programs, but the disease remains endemic in most areas where it has appeared. Because of its rapid spread, high potential for damage, and impact on export sales and domestic trade, citrus canker is a significant threat to all citrus-growing regions.

Australia

The citrus industry is the largest fresh-fruit exporting industry in Australia. Australia has had three outbreaks of citrus canker; two were successfully eradicated and one is ongoing. The disease was found twice during the 1900s in the Northern Territory and was eradicated each time. During the first outbreak in 1912, every citrus tree north of latitude 19° South was destroyed, taking 11 years to eradicate the disease. In 2004, Asiatic citrus canker was detected in an orchard in Emerald, Queensland, and was thought to have occurred from the illegal import of infected citrus plants. The state and federal governments have ordered that all commercial orchards, all non-commercial citrus tress, and all native lime trees (C. glauca) in the vicinity of Emerald be destroyed rather than trying to isolate infected trees.

Brazil

Citrus is an important domestic and export crop for Brazil. Citrus agriculture is the second most important agricultural activity in the Brazilian state of São Paulo, the largest sweet orange production area in the world. There are over 100,000 orchards in São Paulo, and the area planted with citrus is increasing. Of the ~2 million trees, greater than 80% of the trees are a single variety of orange, and the remainder is made up of tangerine and lemon trees. Because of the uniformity in citrus variety the state has been adversely affected by canker, causing crop and monetary losses. In Brazil rather than destroying entire orchards to eradicate the disease contaminated trees and trees within a 30 m radius are destroyed, up to 1998 over half a million trees had been destroyed.

United States

Citrus canker was first found in the United States in 1910 not far from the Georgia - Florida border. Carried by wind and rain, the disease spread southward swiftly, reaching Dade County, more than 400 miles (600 km) away, in just two years. Beyond Florida, the pathogen spread throughout the Gulf States and reached as far north as South Carolina. It took more than 20 years to eradicate that outbreak of citrus canker, from 1914 through 1931, $2.5 million in State and private funds were spent to control it—a sum equivalent to $28 million in 2000 dollars. In 26 counties, some 257,745 grove trees and 3,093,110 nursery trees were destroyed by burning. Citrus Canker was detected again on the Gulf Coast of Florida in 1986 and was successfully eradicated.

The most recent outbreak of Citrus Canker was discovered in Florida in 1995 in a residential area near Miami International Airport. Despite eradication attempts, by late 2005 the disease had spread across much of the state, reaching as far as Orange Park, 315 miles (500 km) away. State agriculture officials indicated that the disease had probably been spread by hurricanes that struck the state in 2004 and 2005. Active eradication programs continue in 13 Florida counties and "sentinel trees" surveys are being conducted across the state to detect new infections

The spread following Hurricane Wilma on October 24, 2005, has been linked to a massive drop in citrus production estimates. A USDA study showed the spread may lead to the destruction of over 183,000 acres (741 km²), which constitutes an added 25 % of total citrus area.