CLED agar
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CLED agar (Cysteine Lactose Electrolyte Deficient medium) is a valuable non-inhibitory growth medium used in the isolation and differentiation of urinary organisms. Being electrolyte deficient, it prevents the swarming of Proteus species. Lactose fermenters produce yellow colonies on CLED agar; non-lactose fermenters appear blue. It has a pH of approximately 7.3. CLED agar contains:
Peptone | 4g/l |
'Lab Lemco' powder | 3g/l |
Tryptone | 4g/l |
Lactose | 10g/l |
L-Cysteine | 128mg/l |
Bromothymol blue | 20mg/l |
Agar No. 1 | 15g/l
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