GRB2-related adapter protein 2 also known as GRB2-related adaptor downstream of Shc (GADS) is a 37 kDaprotein that in humans is encoded by the GRAP2gene.[1][2][3]
This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist.[3]
↑Qiu M, Hua S, Agrawal M, Li G, Cai J, Chan E, Zhou H, Luo Y, Liu M (Dec 1998). "Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1". Biochemical and Biophysical Research Communications. 253 (2): 443–7. doi:10.1006/bbrc.1998.9795. PMID9878555.
↑Xia C, Bao Z, Tabassam F, Ma W, Qiu M, Hua S, Liu M (Jul 2000). "GCIP, a novel human grap2 and cyclin D interacting protein, regulates E2F-mediated transcriptional activity". The Journal of Biological Chemistry. 275 (27): 20942–8. doi:10.1074/jbc.M002598200. PMID10801854.
↑ 5.05.1Ellis JH, Ashman C, Burden MN, Kilpatrick KE, Morse MA, Hamblin PA (Jun 2000). "GRID: a novel Grb-2-related adapter protein that interacts with the activated T cell costimulatory receptor CD28". Journal of Immunology. 164 (11): 5805–14. doi:10.4049/jimmunol.164.11.5805. PMID10820259.
↑ 6.06.1Liu SK, Fang N, Koretzky GA, McGlade CJ (Jan 1999). "The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors". Current Biology. 9 (2): 67–75. doi:10.1016/s0960-9822(99)80017-7. PMID10021361.
↑ 7.07.1Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID16189514.
↑Ma W, Xia C, Ling P, Qiu M, Luo Y, Tan TH, Liu M (Mar 2001). "Leukocyte-specific adaptor protein Grap2 interacts with hematopoietic progenitor kinase 1 (HPK1) to activate JNK signaling pathway in T lymphocytes". Oncogene. 20 (14): 1703–14. doi:10.1038/sj.onc.1204224. PMID11313918.
Liu SK, McGlade CJ (Dec 1998). "Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc". Oncogene. 17 (24): 3073–82. doi:10.1038/sj.onc.1202337. PMID9872323.
Liu SK, Fang N, Koretzky GA, McGlade CJ (Jan 1999). "The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors". Current Biology. 9 (2): 67–75. doi:10.1016/S0960-9822(99)80017-7. PMID10021361.
Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP (Dec 1999). "The DNA sequence of human chromosome 22". Nature. 402 (6761): 489–95. doi:10.1038/990031. PMID10591208.
Xia C, Bao Z, Tabassam F, Ma W, Qiu M, Hua S, Liu M (Jul 2000). "GCIP, a novel human grap2 and cyclin D interacting protein, regulates E2F-mediated transcriptional activity". The Journal of Biological Chemistry. 275 (27): 20942–8. doi:10.1074/jbc.M002598200. PMID10801854.
Zhang W, Trible RP, Zhu M, Liu SK, McGlade CJ, Samelson LE (Jul 2000). "Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell angigen receptor-mediated signaling". The Journal of Biological Chemistry. 275 (30): 23355–61. doi:10.1074/jbc.M000404200. PMID10811803.
Ellis JH, Ashman C, Burden MN, Kilpatrick KE, Morse MA, Hamblin PA (Jun 2000). "GRID: a novel Grb-2-related adapter protein that interacts with the activated T cell costimulatory receptor CD28". Journal of Immunology. 164 (11): 5805–14. doi:10.4049/jimmunol.164.11.5805. PMID10820259.
Ma W, Xia C, Ling P, Qiu M, Luo Y, Tan TH, Liu M (Mar 2001). "Leukocyte-specific adaptor protein Grap2 interacts with hematopoietic progenitor kinase 1 (HPK1) to activate JNK signaling pathway in T lymphocytes". Oncogene. 20 (14): 1703–14. doi:10.1038/sj.onc.1204224. PMID11313918.
Poetsch A, Molday LL, Molday RS (Dec 2001). "The cGMP-gated channel and related glutamic acid-rich proteins interact with peripherin-2 at the rim region of rod photoreceptor disc membranes". The Journal of Biological Chemistry. 276 (51): 48009–16. doi:10.1074/jbc.M108941200. PMID11641407.
Wenzel J, Sanzenbacher R, Ghadimi M, Lewitzky M, Zhou Q, Kaplan DR, Kabelitz D, Feller SM, Janssen O (Dec 2001). "Multiple interactions of the cytosolic polyproline region of the CD95 ligand: hints for the reverse signal transduction capacity of a death factor". FEBS Letters. 509 (2): 255–62. doi:10.1016/S0014-5793(01)03174-X. PMID11741599.
Guyot B, Arnaud S, Phothirath P, Bourette RP, Grasset MF, Rigal D, Mouchiroud G (May 2002). "Genomic organization and restricted expression of the human Mona/Gads gene suggests regulation by two specific promoters". Gene. 290 (1–2): 173–9. doi:10.1016/S0378-1119(02)00555-3. PMID12062812.
Lindholm CK, Henriksson ML, Hallberg B, Welsh M (Jul 2002). "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells". European Journal of Biochemistry / FEBS. 269 (13): 3279–88. doi:10.1046/j.1432-1033.2002.03008.x. PMID12084069.
Zhu M, Janssen E, Leung K, Zhang W (Nov 2002). "Molecular cloning of a novel gene encoding a membrane-associated adaptor protein (LAX) in lymphocyte signaling". The Journal of Biological Chemistry. 277 (48): 46151–8. doi:10.1074/jbc.M208946200. PMID12359715.
Guyot B, Arnaud S, Phothirath P, Rigal D, Mouchiroud G (Dec 2002). "Upstream open reading frames regulate translation of Mona/Gads adapter mRNA in the megakaryocytic lineage". Platelets. 13 (8): 459–64. doi:10.1080/0953710021000059431. PMID12487779.
Yankee TM, Solow SA, Draves KD, Clark EA (Jan 2003). "Expression of the Grb2-related protein of the lymphoid system in B cell subsets enhances B cell antigen receptor signaling through mitogen-activated protein kinase pathways". Journal of Immunology. 170 (1): 349–55. doi:10.4049/jimmunol.170.1.349. PMID12496419.
1r1p: Structural Basis for Differential Recognition of Tyrosine Phosphorylated Sites in the Linker for Activation of T cells (LAT) by the Adaptor Protein Gads
1r1q: Structural Basis for Differential Recognition of Tyrosine Phosphorylated Sites in the Linker for Activation of T cells (LAT) by the Adaptor Protein Gads
1r1s: Structural Basis for Differential Recognition of Tyrosine Phosphorylated Sites in the Linker for Activation of T cells (LAT) by the Adaptor Protein Gads
2d0n: Crystal structure of the C-terminal SH3 domain of the adaptor protein GADS in complex with SLP-76 motif peptide reveals a unique SH3-SH3 interaction
