Ki-67 (Biology)

Antigen identified by monoclonal antibody Ki-67
File:PBB Protein MKI67 image.jpg PDB rendering based on 1r21.
Available structures PDB Ortholog search: Template:Homologene2PDBe PDBe, Template:Homologene2uniprot RCSB List of PDB id codes 1r21​, 2aff​
Identifiers
Symbols	MKI67 ; KIA; Ki-67
External IDs	Template:OMIM5 Template:MGI HomoloGene: 1814
Gene ontology Molecular function Template:GNF GO Template:GNF GO Template:GNF GO Cellular component Template:GNF GO Template:GNF GO Biological process Template:GNF GO Template:GNF GO Template:GNF GO
Orthologs
Template:GNF Ortholog box
Species	Human	Mouse
Entrez	n/a	n/a
Ensembl	n/a	n/a
UniProt	n/a	n/a
RefSeq (mRNA)	n/a	n/a
RefSeq (protein)	n/a	n/a
Location (UCSC)	n/a	n/a
PubMed search	n/a	n/a

File:Mouse NT antibody NF Ki67.jpg

Antibody stain against Neurofilament (green) and Ki 67 (red) in a Mouse embryo 12.5 days after fertilization. The proliferating cells are in the ventricular zone in the neural tube and therefore colored red.

The Ki-67 protein (also known as antigen identified by monoclonal antibody Ki-67or MKI67) is a cellular marker for proliferation. It is strictly associated with cell proliferation. During the interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive tumor cells (the Ki-67 labelling index) is often correlated with the clinical course of cancer. The best-studied examples in this context are carcinomas of the prostate and the breast. For these types of tumors, the prognostic value for survival and tumor recurrence have repeatedly been proven in uni- and multivariate analysis.

The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67 , which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. The name is derived from the city of origin (Kiel) and the number of the original clone in the 96-well plate.

MIB-1 is a commonly used monoclonal antibody that detects the Ki-67 antigen. It is used in clinical applications to determine the Ki-67 labelling index. One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval.

See also

• PCNA - Proliferating Cell Nuclear Antigen, expressed during the DNA synthesis.

Literature

• Scholzen T, Gerdes J (2000). "The Ki-67 protein: from the known and the unknown". J. Cell. Physiol. 182 (3): 311–22. PMID 10653597. (Review)

External links

• Ki-67+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH)