Nucleoporin 85

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Identifiers
Aliases
External IDs	GeneCards: [1]
Orthologs
Species	Human	Mouse
Entrez	n/a	n/a
Ensembl	n/a	n/a
UniProt	n/a	n/a
RefSeq (mRNA)	n/a	n/a
RefSeq (protein)	n/a	n/a
Location (UCSC)	n/a	n/a
PubMed search	n/a	n/a
Wikidata
View/Edit Human

Nucleoporin 85 (Nup85) is a protein that in humans is encoded by the NUP85 gene.

Function

Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160.

Model organisms

Model organisms have been used in the study of NUP85 function. A conditional knockout mouse line called Nup85^{tm1a(KOMP)Wtsi} was generated at the Wellcome Trust Sanger Institute. Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Additional screens performed: - In-depth immunological phenotyping

Nup85 knockout mouse phenotype

Characteristic	Phenotype
All data available at.	Insulin	Normal
Homozygous viability at P14	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Anti-nuclear Antibody Assay	Normal
Epidermal Immune Composition	Normal
Influenza Challenge	Normal

Further reading

• Kapeller R, Toker A, Cantley LC, Carpenter CL (Oct 1995). "Phosphoinositide 3-kinase binds constitutively to alpha/beta-tubulin and binds to gamma-tubulin in response to insulin". The Journal of Biological Chemistry. 270 (43): 25985–91. doi:10.1074/jbc.270.43.25985. PMID 7592789.
• Tynan SH, Purohit A, Doxsey SJ, Vallee RB (Oct 2000). "Light intermediate chain 1 defines a functional subfraction of cytoplasmic dynein which binds to pericentrin". The Journal of Biological Chemistry. 275 (42): 32763–8. doi:10.1074/jbc.M001536200. PMID 10893222.
• Cronshaw JM, Krutchinsky AN, Zhang W, Chait BT, Matunis MJ (Sep 2002). "Proteomic analysis of the mammalian nuclear pore complex". The Journal of Cell Biology. 158 (5): 915–27. doi:10.1083/jcb.200206106. PMC 2173148. PMID 12196509.
• Mulari MT, Patrikainen L, Kaisto T, Metsikkö K, Salo JJ, Väänänen HK (May 2003). "The architecture of microtubular network and Golgi orientation in osteoclasts--major differences between avian and mammalian species". Experimental Cell Research. 285 (2): 221–35. doi:10.1016/S0014-4827(03)00033-8. PMID 12706117.
• Chang F, Re F, Sebastian S, Sazer S, Luban J (Apr 2004). "HIV-1 Vpr induces defects in mitosis, cytokinesis, nuclear structure, and centrosomes". Molecular Biology of the Cell. 15 (4): 1793–801. doi:10.1091/mbc.E03-09-0691. PMC 379276. PMID 14767062.
• Loïodice I, Alves A, Rabut G, Van Overbeek M, Ellenberg J, Sibarita JB, Doye V (Jul 2004). "The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis". Molecular Biology of the Cell. 15 (7): 3333–44. doi:10.1091/mbc.E03-12-0878. PMC 452587. PMID 15146057.
• Terashima Y, Onai N, Murai M, Enomoto M, Poonpiriya V, Hamada T, Motomura K, Suwa M, Ezaki T, Haga T, Kanegasaki S, Matsushima K (Aug 2005). "Pivotal function for cytoplasmic protein FROUNT in CCR2-mediated monocyte chemotaxis". Nature Immunology. 6 (8): 827–35. doi:10.1038/ni1222. PMID 15995708.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Satoh M, Akatsu T, Ishkawa Y, Minami Y, Nakamura M (Mar 2007). "A novel activator of C-C chemokine, FROUNT, is expressed with C-C chemokine receptor 2 and its ligand in failing human heart". Journal of Cardiac Failure. 13 (2): 114–9. doi:10.1016/j.cardfail.2006.11.003. PMID 17395051.