Sandbox ID gallery2

H

Hepatic capillariasis

Capillaria hepatica

Capillaria hepatica eggs

• 
  Eggs of C. hepatica in liver stained with H&E.
  Adapted from CDC

Capillaria hepatica adults

• 
  Cross-section of C. hepatica in liver tissue, stained with H&E. Note the presence of a stichocyte (black arrow) and bacillary bands (blue arrows). Image taken at 200x magnification.
  Adapted from CDC

Heterophyiasis

Heterophyes heterophyes

Adult of Heterophyes heterophyes

• 
  Adult of H. heterophyes, stained with carmine. In this figure, the following structures are labeled: oral sucker (OS), pharynx (PH), intestine (IN), ventral sucker, or acetabulum (AC), and eggs within the uterus (UT)
  Adapted from CDC

Snail intermediate hosts of Heterophyes heterophyes

• 
  Cerithideopsilla cingulata, an intermediate host for H. heterophyes in southeast Asia. Image courtesy of Conchology, Inc, Mactan Island, Philippines
  Adapted from CDC

Hymenolepiasis

Hymenolepis diminuta

Hymenolepis diminuta eggs in wet mounts

• 
  Egg of H. diminuta in an unstained wet mount of concentrated stool. Image taken at 400x magnification.
  Adapted from CDC

Hymenolepis diminuta proglottids

• 
  Proglottids of H. diminuta stained with carmine. Notice the craspedote form of the proglottids.
  Adapted from CDC

Hymenolepis nana

Hymenolepis nana eggs in wet mounts

• 
  Egg of H. nana in an unstained wet mount. In this image, the polar filaments in the space between the oncosphere and outer shell are clearly visible.
  Adapted from CDC

Hymenolepis nana eggs, zinc PVA trichrome stain

• 
  Egg of H. nana in a trichrome-stained stool specimen. Although trichrome is not the preferred method for observing helminth eggs, they can be detected this way. The eggs are distorted, probably due to the zinc polyvinyl alcohol (PVA) used for preserving specimens for trichrome stain. Images courtesy of the Oregon State Public Health Laboratory.
  Adapted from CDC

Hymenolepis nana proglottids

• 
  Higher magnification of the eggs in Figures 1 and 2, taken at 1000x, oil. Hooks do not stain with H&E but are refractile and may be visible in stained specimens with proper adjustment of the microscope. Polar filaments are visible in the egg in the upper right quadrant of the image.
  Adapted from CDC

Hymenolepis nana adults

• 
  Higher magnification of the scolex in Figure 2. In this image, two of the suckers and the rostellar hooks are clearly visible.
  Adapted from CDC

I

Intestinal amebae

Entamoeba coli

E. coli cysts in concentrated wet mounts

• 
  Cyst of E. coli in a concentrated wet mount stained with iodine. Five nuclei are visible in this focal plane.
  Adapted from CDC

E. coli cysts stained with trichrome

• 
  Mature cyst of E. coli, stained with trichrome. This figure and Figure 5 represent the same cyst shown in two different focal planes. Eight nuclei can be seen between the two focal planes.
  Adapted from CDC

E. coli trophozoites stained with trichrome

• 
  Trophozoite of E. coli stained with trichrome.
  Adapted from CDC

Entamoeba gingivalis

E. gingivalis trophozoites stained with trichrome

• 
  Trophozoite of E. gingivalis from culture, stained with trichrome.
  Adapted from CDC

Entamoeba hartmanni

E. hartmanni cyst in a wet mount

• 
  Cyst of an E. hartmanni in a wet mount, stained with iodine.
  Adapted from CDC

E. hartmanni cysts stained with trichrome

• 
  Cyst of E. hartmanni stained with trichrome.
  Adapted from CDC

E. hartmanni trophozoites stained with trichrome

• 
  Trophozoite of E. hartmanni stained with trichrome.
  Adapted from CDC

Entamoeba polecki

E. polecki cyst in a concentrated wet mount, stained with iodine

• 
  Cyst of E. polecki in a wet mount, stained with iodine. Notice the numerous chromatoid bodies (arrows).
  Adapted from CDC

E. polecki cysts stained with trichrome

• 
  Cyst of E. polecki stained with trichrome. Notice the large nucleus with a pleomorphic karyosome and numerous variably-shaped chromatoid bodies.
  Adapted from CDC

E. polecki trophozoites stained with trichrome

• 
  Trophozoite of E. polecki stained with trichrome.
  Adapted from CDC

Endolimax nana

Endolimax nana cysts in concentrated wet mounts

• 
  Cyst of E. nana in a direct wet mount stained with iodine.
  Adapted from CDC

E. nana cyst stained with trichrome

• 
  Cyst of E. nana stained with trichrome.
  Adapted from CDC

E. nana trophozoites stained with trichrome

• 
  Trophozoite of E. nana stained with trichrome.
  Adapted from CDC

Iodamoeba buetschlii

Iodamoeba buetschlii cysts in concentrated wet mounts

• 
  Cyst of I. buetschlii from the same specimen as seen in Figures A and B, but stained with iodine. In this cyst, the glycogen vacuole is more-easily observed as a dark-staining mass in the cyst.
  Adapted from CDC

I. buetschlii cysts stained with trichrome

• 
  Cyst of I. buetschlii stained with trichrome. In this specimen, both the nucleus and large glycogen vacuole are visible.
  Adapted from CDC

I. buetschlii trophozoite stained with trichrome

• 
  Trophozoite of I. buetschlii trophozoite stained with trichrome.
  Adapted from CDC

Intestinal capillariasis

Capillaria philippinensis

Capillaria philippinensis eggs

• 
  Egg of C. philippinensis in an unstained wet mount of stool.
  Adapted from CDC

Capillaria philippinensis adults

• 
  Cross-section of a gravid adult female C. philippinensis from an intestinal biopsy specimen, stained with H&E. Shown in this figure are a bacillary band (blue arrow), the intestine (red arrow) and uterus containing an egg in cross-section (black arrow).
  Adapted from CDC

L

Leishmaniasis (Visceral leishmaniasis, Kala-azar)

Leishmania sp.

Leishmania amastigotes

• 
  Leishmania tropica amastigotes from an impression smear of a biopsy specimen from a skin lesion. In this figure, amastigotes are being freed from a rupturing macrophage. Patient had traveled to Egypt, Africa, and the Middle East. Based on culture in NNN medium, followed by isoenzyme analysis, the species was identified as L. tropica.
  Adapted from CDC

Leishmania mexicana in tissue stained with hematoxylin and eosin (H&E)

• 
  Leishmania mexicana in a biopsy specimen from a skin lesion stained with H&E. The amastigotes are lining the walls of two vacuoles, a typical arrangement. The species identification was derived from culture followed by isoenzyme analysis.
  Adapted from CDC

Leishmania sp. promastigotes from culture

• 
  Leishmania sp. promastigotes from culture.
  Adapted from CDC

Loaiasis

Loa loa

Microfilariae of Loa loa

• 
  Higher magnification of the microfilariae in Figure 7, taken at 500x oil magnification.
  Adapted from CDC

Adults of L. loa

• 
  Adult of L. loa removed from the eye of a patient. Image courtesy of the Georgia State Public Health Laboratory.
  Adapted from CDC

Lymphatic filariasis (Bancroftian filariasis)

Brugia malayi

• 
  Microfilaria of B. malayi in a thin blood smear, stained with Giemsa.
  Adapted from CDC

Brugia timori

• 
  Microfilaria of B. timori in a thick blood smear from a patient from Indonesia, stained with Giemsa and captured at 500x oil magnification. Image from a specimen courtesy of Dr. Thomas C. Orihel, Tulane University, New Orleans, LA.
  Adapted from CDC

Wuchereria bancrofti

Microfilariae of Wuchereria bancrofti

• 
  Close-up of the posterior end of the worm in Figure 3.
  Adapted from CDC

Adults of W. bancrofti

• 
  Adults of W. bancrofti. The male worm is on the left; the female is on the right.
  Adapted from CDC

M

Mansonellosis

Mansonella ozzardi

Microfilariae of Mansonella ozzardi

• 
  Microfilaria of M. ozzardi in a thick blood smear, stained with Giemsa. Note the hook-like end to the tail in this figure.
  Adapted from CDC

Mansonella perstans

Microfilariae of Mansonella perstans

• 
  Microfilaria of M. perstans in a thick blood smear stained with Giemsa. Image courtesy of the Parasitology Department, Public Health Lab, Ontario Agency for Health Protection and Promotion, Canada.
  Adapted from CDC

Mansonella streptocerca

Microfilariae of Mansonella streptocerca

• 
  Microfilaria of M. streptocerca, fixed in 2% formalin and stained with hematoxylin.
  Adapted from CDC

Mesocestoidiasis

Mesocestoides spp.

Mesocestoides spp. proglottids and scoleces

• 
  Scolex of Mesocestoides sp. stained with carmine. In this field, two of the suckers are clearly visible. Note that lack of rostellar hooklets.
  Adapted from CDC

Mesocestoides spp. tetrathyridia

• 
  Tetrathyridium of Mesocestoides sp. in the liver of a laboratory-infected mouse.
  Adapted from CDC

Metagonimiasis

Metagonimus yokogawai

Metagonimus yokogawai, adult fluke

• 
  Adult M. yokogawai, stained with carmine. In this figure, the following structures are labeled: oral sucker (OS), pharynx (PH), intestine (IN), genitoacetabulum (GA), ovary (OV), the large, paired testes (TE), and eggs within the uterus (EG).
  Adapted from CDC

Snail intermediate hosts of M. yokogawai

• 
  Snail in the genus, Semisulcospira. Image courtesy of Conchology, Inc, Mactan Island, Philippines.
  Adapted from CDC

Microsporidiosis

Encephalitozoon cuniculi

• 
  Spores of E. cuniculi in a kidney biopsy specimen stained with Ryan's modified trichrome.
  Adapted from CDC

Encephalitozoon hellem

• 
  Monoclonal antibody-based immunofluorescence identification of Encephalitozoon hellem.
  Adapted from CDC

Encephalitozoon intestinalis

• 
  Encephalitozoon intestinalis stained with Calcofluor white.
  Adapted from CDC

Enterocytozoon bieneusi

• 
  Enterocytozoon bieneusi spores stained with Chromotrope 2R.
  Adapted from CDC

Myiasis (Bot Flies)

Cuterebra spp.

• 
  Adult of Cuterebra sp. Cuterebra spp. are primarily parasites of rodents and lagomorphs, although human infections are rare. Images taken from specimens courtesy of the Georgia Museum of Natural History, University of Georgia, Athens, GA.
  Adapted from CDC

Dermatobia hominis

• 
  Adult of Dermatobia hominis, the human bot fly. Image taken from a specimen courtesy of the Georgia Museum of Natural History, University of Georgia, Athens, GA.
  Adapted from CDC

Oestrus ovis

• 
  First instar larva of O. ovis, collected from the eye of a patient in India presenting with conjunctivitis. Image courtesy of the L V Prasad Eye Institute, Banjara Hills, India.
  Adapted from CDC

Phormia regina

• 
  Close-up of the posterior spiracles of the specimen in Figure 1.
  Adapted from CDC

O

Oesophagostomiasis

Oesophagostomum spp.

Eggs of Oesophagostomum spp.

• 
  Egg of Oesophagostomum sp. in an unstained wet mount of stool.
  Adapted from CDC

L3 infective larvae of Oesophagostomum spp.

• 
  Tail-end of the specimen in Figures 1-3. Notice the long tail space (arrow) and long, tapering tail sheath.
  Adapted from CDC

Adults of Oesophagostomum spp.

• 
  Same specimen as in Figure 5, but shown in a slightly different focal plane. Note the bursa (BU).
  Adapted from CDC

Oesophagostomum spp. in tissue specimens

• 
  Higher-magnification (200x) of the specimen in Figure 1. Note the large, platymyarian muscle cells (MU) and thick, muscled esophagus (ES).
  Adapted from CDC

Onchocerciasis (River Blindness)

Onchocerca volvulus

Microfilariae of Onchocerca volvulus in tissue

• 
  Microfilariae of O. volvulus from a skin nodule of a patient from Zambia, stained with hematoxylin and eosin (H&E). Image taken at 1000x oil magnification. Microfilariae of O. volvulus within the uterus of an adult female. The specimen was taken from the same patient as in Figure 1. Image taken at 500x magnification, oil.
  Adapted from CDC
• 
  Cross-section of an adult female O. volvulus, stained with H&E. Note the presence of many microfilariae within the uterus.
  Adapted from CDC

Adults of Onchocerca volvulus in tissue

• 
  Cross-section of an adult female Onchocerca sp. from the biopsy of a scalp nodule from a patient from Liberia. Note the presence of the intestine (blue arrow), uterine tubes (red arrows) and some cuticular nodules (green arrows). Also notice the weak musculature under the thick cuticle. Image courtesy of Drs. Philip LeBoit and Paul Borbeau.
  Adapted from CDC

Opisthorchiasis

Opisthorchis felineus

Adults of Opisthorchis felineus

• 
  Adult of O. felineus. Image courtesy of the Web Atlas of Medical Parasitology and the Korean Society for Parasitology.
  Adapted from CDC

Intermediate hosts of Opisthorchis spp.

• 
  Bithynia sp., a common intermediate host of Opisthorchis spp. Image courtesy of Michal Manas.
  Adapted from CDC

Opisthorchis viverrini

Eggs of Opisthorchis viverrini in wet mounts

• 
  Egg of O. viverrini in an unstained wet mount of concentrated stool. Image taken at 400x magnification.
  Adapted from CDC

Adults of O. viverrini

• 
  Adult of O. viverrini. Image courtesy of the Web Atlas of Medical Parasitology and the Korean Society for Parasitology.
  Adapted from CDC

P

Paragonimiasis

Paragonimus spp.

Eggs of Paragonimus spp. in unstained wet mounts

• 
  Egg of P. westermani in an unstained wet mount.
  Adapted from CDC

Eggs of Paragonimus spp. in tissue

• 
  Egg of Paragonimus sp. taken from a lung biopsy stained with hematoxylin and eosin (H&E). This egg measured 80-90 µm by 40-45 µm. The species was not identified in this case.
  Adapted from CDC

Eggs of Paragonimus kellicotti

• 
  Higher magnification (1000x, oil) of the specimen in Figures 3 and 4. Image courtesy of Dr. Gary Procop.
  Adapted from CDC

Adults of Paragonimus spp.

• 
  Sections of several adults of P. kellicotti taken from a pleural biopsy stained with H&E. Numbers 1-5 show the individual worms. The integument is gone from most of the worms, as this was probably an old infection, but remnants of the gonad (A, dart) and uterine tubes (B) can be seen. Image courtesy of Dr. Miguel Madariaga, University of Nebraska Medical Center. Image first appeared in: Madariaga, MD, M. G., T. Ruma, MD, and J. H. Theis, MD. 2007. Autochthonous human paragonimiasis in North America. Wilderness & Environmental Medicine. 18(3): 203-205. Image used with permission of © Allen Press Publishing Services.
  Adapted from CDC

Pediculosis

Pediculosis sp.

Head and Body Lice adults

• 
  Adult of P. humanus.
  Adapted from CDC

Head and Body Lice nits

• 
  Empty shell of the nit in Figure 1, the nymph having left.
  Adapted from CDC

Pentatrichomonas hominis

Pentatrichomonas hominis trophozoites

• 
  Trophozoite of P. hominis in a stool specimen, stained with iron hematoxylin.
  Adapted from CDC

Philophthalmiasis

Philophthalmus spp.

Philophthalmus spp, adult flukes

• 
  Close-up of the anterior end of the worm in Figure 3, showing a close-up of the oral sucker (OS), pharynx (PH), and acetabulum (AC).
  Adapted from CDC

Snail intermediate hosts of Philophthalmus spp.

• 
  Snails thyridium of Mesocestoides sp. in the liver of a laboratory-infected mouse.
  Adapted from CDC

Pneumocystis pneumonia (PCP)

Pneumocystis jirovecii (previously Pneumocystis carinii)

Pneumocystis jirovecii trophozoites

• 
  Trophozoites of P. jirovecii in a bronchoalveolar lavage (BAL) specimen from an AIDS patient, stained with Giemsa.
  Adapted from CDC

Pneumocystis jirovecii cysts

• 
  Cysts of P. jirovecii in lung tissue, stained with methenamine silver and hematoxylin and eosin (H&E). The walls of the cysts are stained black; the intracystic bodies are not visible with this stain.
  Adapted from CDC

Indirect immunofluorescence using monoclonal antibodies against Pneumocystis jirovecii

• 
  Direct immunofluorescence antibody stain using monoclonal antibodies that target Pneumocystis jirovecii. This image is from a bronchoalveolar lavage (BAL) specimen from a patient with a malignancy. Image courtesy of Brigham & Women's Hospital, Boston, MA.
  Adapted from CDC

R

Retortamonas intestinalis

Retortamonas intestinalis, trophozoites

• 
  Trophozoite of R. intestinalis in a stool specimen, stained with trichrome.
  Adapted from CDC

Retortamonas intestinalis, cysts

• 
  Cyst of R. intestinalis in a stool specimen, stained with trichrome.
  Adapted from CDC

S

Sarcocystosis

Sarcocystis spp.

Sarcocystis oocysts in wet mounts

• 
  Sporulated oocyst of Sarcocystis sp. in unstained wet mounts, magnification 400x.
  Adapted from CDC

Sarcocystis oocysts in wet mounts viewed under differential interference contrast (DIC)

• 
  Sporulated oocyst of Sarcocystis sp. in a wet mount viewed under DIC microscopy, magnification 400x.
  Adapted from CDC

Sarcocystis oocysts in wet mounts viewed under ultraviolet (UV) microscopy

• 
  Individual sporocyst of Sarcocystis sp. in a wet mount viewed under UV microscopy, magnification 400x.
  Adapted from CDC

Sarcocystis sarcocysts in tissue

• 
  Higher magnification (1000x) of the sarcocyst in Figure 5, showing many bradyzoites.
  Adapted from CDC

Schistosomiasis (Bilharziasis)

Schistosoma haematobium

• 
  Egg of S. haematobium in a wet mount of a urine concentrate.
  Adapted from CDC

Schistosoma intercalatum

• 
  Egg of S. intercalatum in a wet mount.
  Adapted from CDC

Schistosoma japonicum

• 
  Egg of S. japonicum in an unstained wet mount. Note the small, inconspicuous spines (red arrows).
  Adapted from CDC
• 
  Egg of S. japonicum in an unstained wet mount of stool.
  Adapted from CDC

Schistosoma mansoni

• 
  Adults of S. mansoni. The thin female resides in the gynecophoral canal of the thicker male. Note the tuberculate exterior of the male.
  Adapted from CDC

Schistosoma mekongi

• 
  Egg of S. mekongi. Note the inconspicuous spine (red arrow).
  Adapted from CDC

Sparganosis

Proliferating spargana in groin tissue

• 
  Proliferating sparganum in groin tissue of a patient from Paraguay, stained with hematoxylin and eosin (H&E).
  Adapted from CDC

Proliferating spargana in lung tissue

• 
  Higher magnification of the sparganum in Figure 3. In this image, calcareous corpuscles (green arrows) can be seen.
  Adapted from CDC

Spargana removed from tissue

• 
  Sparganum removed from the ocular conjunctiva of a patient from Taiwan. The worm measured 40 mm long. Image courtesy of Dr. John H. Cross and the Uniformed Services University of the Health Sciences, Bethesda, MD.
  Adapted from CDC

Strongyloidiasis

Strongyloides stercoralis

Strongyloides stercoralis first-stage rhabditiform (L1) larvae

• 
  Rhabditiform larva of S. stercoralis in an unstained wet mount of stool. Notice the prominent genital primordium (blue arrow), rhabditoid esophagus (red arrow) and short buccal canal (green arrow).
  Adapted from CDC

Strongyloides stercoralis third-stage filariform (L3) larvae

• 
  Higher magnification (1000x oil) of the worm in Figure 2. Notice the notched tail.
  Adapted from CDC

Strongyloides stercoralis free-living adults

• 
  Adult free-living female S. stercoralis. Notice the row of eggs within the female’s body.
  Adapted from CDC

Strongyloides stercoralis in tissue

• 
  Longitudinal-section of a larva of S. stercoralis from the same specimen as Figure 5. Image taken at 400x magnification.
  Adapted from CDC

T

Taeniasis

Taenia spp.

Taenia spp. eggs

• 
  Unstained Taenia sp. egg, teased from a proglottid of an adult. Four hooks can easily be seen in this image.
  Adapted from CDC

Taenia spp. scoleces

• 
  Scolex of T. saginata. Note the four large suckers and lack of rostellum and rostellar hooks.
  Adapted from CDC

Taenia spp. proglottids

• 
  The same proglottid as in Figure 5 injected with India ink, demonstrating the number of primary uterine branches (>12). Image courtesy of the Oregon State Public Health Laboratory.
  Adapted from CDC

Cross-sections of Taenia spp. stained with hematoxylin and eosin (H&E)

• Close-up of a cross-section of a Taenia sp. proglottid stained with H&E, showing numerous calcareous corpuscles. Image courtesy of Ameripath. Adapted from CDC
  Close-up of a cross-section of a Taenia sp. proglottid stained with H&E, showing numerous calcareous corpuscles. Image courtesy of Ameripath.
  Adapted from CDC

Taenia spp. adults

• Taenia saginata adult worm. Adapted from CDC
  Taenia saginata adult worm.
  Adapted from CDC

Thelaziasis

Thelazia spp.

Thelazia spp. adults

• 
  Posterior end of a female Thelazia sp.
  Adapted from CDC

Intermediate hosts of Thelazia spp.

• 
  Musca domestica, the house fly. This species has been implicated in the transmission of thelaziasis in the United States and Asia. Image courtesy of Parasite and Diseases Image Library, Australia.
  Adapted from CDC

Toxocariasis

Toxocara canis

Toxocara canis larva hatching

• 
  T. canis larva.
  Adapted from CDC

Toxocara cati

Adult Toxocara cati worms

• 
  Close-up of the posterior end of T. cati, showing a prominent point at the end of the “tail.”.
  Adapted from CDC

Trichinellosis (Trichinosis)

Trichinella spp.

Encysted larvae of Trichinella in tissue, stained with hematoxylin and eosin (H&E)

• Higher-magnification of the larvae in Figure 3. Shown in these cuts are a nucleated stichocyte (ST), prominent lateral chords, or bacillary bands, (LC), immature reproductive tubes (RT), and the intestine (IN). Image captured at 1000x magnification. Adapted from CDC
  Higher-magnification of the larvae in Figure 3. Shown in these cuts are a nucleated stichocyte (ST), prominent lateral chords, or bacillary bands, (LC), immature reproductive tubes (RT), and the intestine (IN). Image captured at 1000x magnification.
  Adapted from CDC

Trichinella larvae in tongue tissue of a rat, stained with H&E

• 
  Trichinella larva in tongue muscle of a rat, stained with hematoxylin and eosin (H&E). Image was captured at 400x magnification.
  Adapted from CDC

Larvae of Trichinella from bear meat

• 
  Larva of Trichinella liberated from bear meat. This larva is from a different case than those shown in Figures 1-4.
  Adapted from CDC

Trichostrongylosis

Trichostrongylus spp.

Trichostrongylus spp. eggs in wet mounts

• 
  Egg of Trichostrongylus sp. in an unstained wet mount of stool. Image courtesy of the Indiana State Department of Health.
  Adapted from CDC

Trichostrongyle eggs in wet mounts

• 
  Egg of a trichostrongyle from the same specimen as Figures 1 and 2. In this egg, a developing larva can be observed.
  Adapted from CDC

Trichostrongylus adults

• 
  Posterior end of a male Trichostrongylus sp. Note the presence of a bursa (red arrow) and spicule (blue arrow). of a glycerin-mounted specimen, taken at 200x magnification.
  Adapted from CDC

Tungiasis

Tunga penetrans

Tunga penetrans

• 
  Close-up of the eggs from the specimen in Figure 2.
  Adapted from CDC

Tunga penetrans lesions and biopsy specimens

• 
  Cross-sections of T. penetrans in tissue, stained with hematoxylin and eosin (H&E). In this image, the following structures are labeled: cuticle (CU), gut (GU), and developing eggs (EG).
  Adapted from CDC