Severe acute respiratory syndrome other diagnostic studies
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Differentiating Severe Acute Respiratory Syndrome from other Diseases |
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
Other Diagnostic Studies
With the identification and sequencing of the RNA of the coronavirus responsible for SARS on April 12, 2003, several diagnostic test kits have been produced and are now being tested for their suitability for use.
Three possible diagnostic tests have emerged, each with drawbacks. The first, an ELISA (enzyme-linked immunosorbent assay) test detects antibodies to SARS reliably but only 21 days after the onset of symptoms. The second, an immunofluorescence assay, can detect antibodies 10 days after the onset of the disease but is a labour and time intensive test, requiring an immunofluorescence microscope and an experienced operator. The last test is a PCR (polymerase chain reaction) test that can detect genetic material of the SARS virus in specimens ranging from blood, sputum, tissue samples and stools. The PCR tests so far have proven to be very specific but not very sensitive. This means that while a positive PCR test result is strongly indicative that the patient is infected with SARS, a negative test result does not mean that the patient does not have SARS.
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![TEM from a tissue culture isolate, revealing numbers of severe acute respiratory virus (SARS) virions. From Public Health Image Library (PHIL). [1]](/images/4/4a/Coronavirus15.jpeg)
TEM from a tissue culture isolate, revealing numbers of severe acute respiratory virus (SARS) virions. From Public Health Image Library (PHIL). [1]
![TEM from a tissue culture isolate, revealing numbers of severe acute respiratory virus (SARS) virions. From Public Health Image Library (PHIL). [1]](/index.php/File:Coronavirus15.jpeg)