Tolonium chloride
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Template:Chembox new Tolonium chloride (INN, also known as toluidine blue O) is a compound used in histology.
Test for lignin
Toluidine blue solution is used in testing for lignin, a complex organic molecule that bonds to cellulose fibres and strengthens and hardens the cell walls in plants. A positive toluidine blue test causes the solution to turn from blue to blue-green. A similar test can be performed with phloroglucinol-HCl solution, which turns red.
Toluidine Blue is often used to identify Mast cells. It is also used to stain proteoglycans and glycosaminoglycans in tissues such as cartilage.