Chancroid pathophysiology: Difference between revisions
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==Overview== | ==Overview== | ||
Chancroid may develop after transmission of ''Haemophilus ducreyi'' through breaks in human epithelium, most commonly through sexual contact. Fimbrialike proteins, Flp1, Flp2, Flp3, are suspected to form pili that assist in adhesion and microcolony formation. ''H. ducreyi'' induces secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8), which causes inflammatory cells to form abscesses, leading to the formation of papules that may progress into pustules. ''H. ducreyi'' cytolethal distending toxin (HdCDT) is a major virulence factor that contributes to necrosis of myeloid and epithelial cells, causing ulceration. On gross examination, pastules, pustules, and ulcers and charactersitic features, depending on the stage of the disease. On microscopic examination, a three-zone structure is typical. | |||
==Pathophysiology== | ==Pathophysiology== | ||
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*Chancroid may develop after transmission of class I or class II of the bacterium ''Haemophilus ducreyi'' through sexual contact.<ref name="ChancroidMedlinePlus">Chancroid. MedlinePlus (Decemner 2, 2015). https://www.nlm.nih.gov/medlineplus/ency/article/000635.htm Accessed January 6, 2015.</ref> | *Chancroid may develop after transmission of class I or class II of the bacterium ''Haemophilus ducreyi'' through sexual contact.<ref name="ChancroidMedlinePlus">Chancroid. MedlinePlus (Decemner 2, 2015). https://www.nlm.nih.gov/medlineplus/ency/article/000635.htm Accessed January 6, 2015.</ref> | ||
*A class I gentically distinct subclade strain of ''H. ducreyi'' may serve as the etiologic agent of non-sexually transmitted skin ulcers.<ref name="pmid25271477">{{cite journal| author=Marks M, Chi KH, Vahi V, Pillay A, Sokana O, Pavluck A et al.| title=Haemophilus ducreyi associated with skin ulcers among children, Solomon Islands. | journal=Emerg Infect Dis | year= 2014 | volume= 20 | issue= 10 | pages= 1705-7 | pmid=25271477 | doi=10.3201/eid2010.140573 | pmc=PMC4193279 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=25271477}}</ref><ref name="pmid25774793">{{cite journal| author=Gaston JR, Roberts SA, Humphreys TL| title=Molecular phylogenetic analysis of non-sexually transmitted strains of Haemophilus ducreyi. | journal=PLoS One | year= 2015 | volume= 10 | issue= 3 | pages= e0118613 | pmid=25774793 | doi=10.1371/journal.pone.0118613 | pmc=PMC4361675 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=25774793 }} </ref> | *A class I gentically distinct subclade strain of ''H. ducreyi'' may serve as the etiologic agent of non-sexually transmitted skin ulcers.<ref name="pmid25271477">{{cite journal| author=Marks M, Chi KH, Vahi V, Pillay A, Sokana O, Pavluck A et al.| title=Haemophilus ducreyi associated with skin ulcers among children, Solomon Islands. | journal=Emerg Infect Dis | year= 2014 | volume= 20 | issue= 10 | pages= 1705-7 | pmid=25271477 | doi=10.3201/eid2010.140573 | pmc=PMC4193279 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=25271477}}</ref><ref name="pmid25774793">{{cite journal| author=Gaston JR, Roberts SA, Humphreys TL| title=Molecular phylogenetic analysis of non-sexually transmitted strains of Haemophilus ducreyi. | journal=PLoS One | year= 2015 | volume= 10 | issue= 3 | pages= e0118613 | pmid=25774793 | doi=10.1371/journal.pone.0118613 | pmc=PMC4361675 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=25774793 }} </ref> | ||
*''H. ducreyi'' is an obligate human pathogen. | |||
===Pathogenesis=== | ===Pathogenesis=== | ||
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Yazan Daaboul, M.D.; Nate Michalak, B.A.; Serge Korjian M.D.
Overview
Chancroid may develop after transmission of Haemophilus ducreyi through breaks in human epithelium, most commonly through sexual contact. Fimbrialike proteins, Flp1, Flp2, Flp3, are suspected to form pili that assist in adhesion and microcolony formation. H. ducreyi induces secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8), which causes inflammatory cells to form abscesses, leading to the formation of papules that may progress into pustules. H. ducreyi cytolethal distending toxin (HdCDT) is a major virulence factor that contributes to necrosis of myeloid and epithelial cells, causing ulceration. On gross examination, pastules, pustules, and ulcers and charactersitic features, depending on the stage of the disease. On microscopic examination, a three-zone structure is typical.
Pathophysiology
Transmission
- Chancroid may develop after transmission of class I or class II of the bacterium Haemophilus ducreyi through sexual contact.[1]
- A class I gentically distinct subclade strain of H. ducreyi may serve as the etiologic agent of non-sexually transmitted skin ulcers.[2][3]
- H. ducreyi is an obligate human pathogen.
Pathogenesis
- H. ducreyi enters the skin through breaks in the epithelium.
- H. ducreyi produces 3 fimbrialike proteins (Flp), Flp1, Flp2, and Flp3 that help the bacteria adhere to subcutaneous epithelial cells and fibroblasts.[4][5]
- H. ducreyi recruits inflammatory cells to the infected area and induces secretion of interleukin 6 (IL-6) and interleukin 8 (IL-8) from epithelial cells. IL-8 induces neutrophils and macrophages to form abscesses, which may cause the presentation of erythematous papules which progress into intradermal pustules.[5][6][7]
- Ulcers develop after secretion of the virulence factor H. ducreyi cytolethal distending toxin (HdCDT), which causes necrosis of myeloid and epithelial cells.[7]
- It is presumed that iron plays an essential role in chancroid pathogenesis.[5]
- H. ducreyi has the ability to avoid phagocytosis.[7]
Virulence Factors
- H. Ducreyi produces and secretes 2 major virulence factors: fimbrialike protein (Flp) and H. ducreyi cytolethal distending toxin (HdCDT).
- Flp:
- A 12.8 kb Flp operon regulates the expression of 3 fimbrialike proteins, Flp1, Flp2, and Flp3 in a type IV secretion system. These proteins have been demonstrated to play an important role in adherence to fibroblasts and pathogenesis of chancroid.[4]
- HdCDT:[8]
- HdCDT is a tripartite protein complex consisting of CdtA, CdtB, and CdtC subunits, all of which are required to produce the active form of the toxin.
- HdCDT activates DNase, inducing DNA double-stranded breaks that result in cellular responses similar to that of ionizing radiation.
- The effect of HdCDT is cell type-specific.
- In epithelial cells and fibroblasts, DNA damage activates ATM kinases, which activate RhoA GTPase leading to induction of actin stress fibers and cell distention.
- RhoA activation is not detected in lymphocytes.
- Flp:
- Other virulence factors that have been isolated include the following:[9]
- Lipooligosaccharide
- Hemolysin
- Copper-zinc superoxide dismutase
- Outer membrane proteins (OMP) DsrA, PAL, and hemoglobin-binding OMP
Adhesion
- The tadA gene is though to be an important regulator for expression of the flp gene cluster.
- Flp1, Flp2, and Flp3 are suspected to play a role in forming pili on the cellular surface of H. ducreyi.
- In vitro, animal, and human models demonstrate that Flps are necessary for H. ducreyi to form microcolonies, which enables pathogenesis.[4]
Gross Pathology
On gross pathology, the following characteristic features may be present in chancroid:
- Erythematous papules
- Intraepidermal pustules
- Soft (nonindurated) ulcers with irregular borders and sharp margins.
Microscopic Pathology
On microscopic pathology, the following characteristic features may be present in chancroid:
- Three-zone structure:
- Narrow superficial zone - contains necrotic tissue, erythrocytes, fibrin, and degenerated leucocytes
- Middle zone - contains edematous inflamed tissue
- Deep zone - contains plasma cells and lymphocytes
- Granulomatous base
- Purulent exudates
References
- ↑ Chancroid. MedlinePlus (Decemner 2, 2015). https://www.nlm.nih.gov/medlineplus/ency/article/000635.htm Accessed January 6, 2015.
- ↑ Marks M, Chi KH, Vahi V, Pillay A, Sokana O, Pavluck A; et al. (2014). "Haemophilus ducreyi associated with skin ulcers among children, Solomon Islands". Emerg Infect Dis. 20 (10): 1705–7. doi:10.3201/eid2010.140573. PMC 4193279. PMID 25271477.
- ↑ Gaston JR, Roberts SA, Humphreys TL (2015). "Molecular phylogenetic analysis of non-sexually transmitted strains of Haemophilus ducreyi". PLoS One. 10 (3): e0118613. doi:10.1371/journal.pone.0118613. PMC 4361675. PMID 25774793.
- ↑ 4.0 4.1 4.2 Spinola, S. M.; Fortney, K. R.; Katz, B. P.; Latimer, J. L.; Mock, J. R.; Vakevainen, M.; Hansen, E. J. (2003). "Haemophilus ducreyi Requires an Intact flp Gene Cluster for Virulence in Humans". Infection and Immunity. 71 (12): 7178–7182. doi:10.1128/IAI.71.12.7178-7182.2003. ISSN 0019-9567.
- ↑ 5.0 5.1 5.2 Abeck D, Johnson AP (1992). "Pathophysiological concept of Haemophilus ducreyi infection (chancroid)". Int J STD AIDS. 3 (5): 319–23. PMID 1391058.
- ↑ Chancroid. Wikipedia (July 16, 2015). https://en.wikipedia.org/wiki/Chancroid Accessed on January 6, 2016.
- ↑ 7.0 7.1 7.2 Chancroid in Emergency Medicine. Medscape (February 12, 2014). http://emedicine.medscape.com/article/781520-overview#a5 Accessed January 8, 2016.
- ↑ Frisan, Teresa; Cortes-Bratti, Ximena; Chaves-Olarte, Esteban; Stenerlow, Bo; Thelestam, Monica (2003). "The Haemophilus ducreyi cytolethal distending toxin induces DNA double-strand breaks and promotes ATM-dependent activation of RhoA". Cellular Microbiology. 5 (10): 695–707. doi:10.1046/j.1462-5822.2003.00311.x. ISSN 1462-5814.
- ↑ Nika, J. R. (2002). "Haemophilus ducreyi Requires the flp Gene Cluster for Microcolony Formation In Vitro". Infection and Immunity. 70 (6): 2965–2975. doi:10.1128/IAI.70.6.2965-2975.2002. ISSN 0019-9567.