Zika virus infection laboratory findings: Difference between revisions
Jump to navigation
Jump to search
YazanDaaboul (talk | contribs) No edit summary |
YazanDaaboul (talk | contribs) |
||
| Line 23: | Line 23: | ||
===PCR=== | ===PCR=== | ||
*During the first week after onset of symptoms, Zika virus may be diagnosed by performing reverse transcriptase-polymerase chain reaction (RT-PCR) on serum. | *During the first week after onset of symptoms, Zika virus may be diagnosed by performing reverse transcriptase-polymerase chain reaction (RT-PCR) on serum. | ||
==References== | ==References== | ||
Revision as of 15:43, 27 January 2016
|
Zika virus infection Microchapters |
|
Diagnosis |
|
Treatment |
|
Case Studies |
|
Zika virus infection laboratory findings On the Web |
|
American Roentgen Ray Society Images of Zika virus infection laboratory findings |
|
Risk calculators and risk factors for Zika virus infection laboratory findings |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Yazan Daaboul, M.D.; Nate Michalak, B.A.; Serge Korjian M.D.
Overview
The diagnosis of Zika virus infection is usually made by detection of elevated IgM and IgG Zika virus antibodies by ELISA or viral RNA by RT-PCR. Non-specific lab findings include elevated markers of inflammation, mild neutropenia, normal leukocyte count or mild leukocytosis with normal platelet count and liver function tests. State health departments are requested to report laboratory-confirmed Zika virus infections to CDC. Pregnant women suspected to have Zika virus infection may be required to undergo amniocentesis and testing of histopathologic samples of the placenta and umbilical cord, frozen placental tissue and cord tissue for Zika virus RNA, and cord serum for Zika and dengue virus IgM and neutralizing antibodies.
Laboratory Findings
- State health departments are requested to report laboratory-confirmed Zika virus infections to CDC.
- The diagnosis of Zika virus infection is usually made by detection of elevated IgM and IgG Zika virus antibodies by ELISA or viral RNA by RT-PCR.
- Patients with Zika virus may also have the following non-specific lab findings
Non-Specific Laboratory Findings
- Elevated markers of inflammation and acute phase reactants (e.g. ESR, C-reactive protein, ferritin)
- Elevated lactate dehydrogenase (LDH)
- Mild neutropenia
- Normal leukocyte count / mild leukocytosis
- Platelet count and liver function tests (LFTs) are usually normal (vs. thrombocytopenia and elevated LFTs in Dengue fever)
ELISA
- ELISA can be used to detect virus-specific IgM (anti-ZIKV-IgM) and neutralizing antibodies toward the end of the first week of illness.
- Cross-reaction with related flaviviruses (e.g., dengue and West Nile viruses) is common and may be difficult to discern.
- Plaque-reduction neutralization testing can be performed to measure virus-specific neutralizing antibodies and discriminate between cross-reacting antibodies in primary flavivirus infections.[1]
PCR
- During the first week after onset of symptoms, Zika virus may be diagnosed by performing reverse transcriptase-polymerase chain reaction (RT-PCR) on serum.
References
- ↑ For HealthCare Providers: Diagnostic Testing. Center for Disease Control and Prevention (June 1, 2015). http://www.cdc.gov/zika/hc-providers/diagnostic.html Accessed December 21, 2015.