Amoebic liver abscess other diagnostic studies: Difference between revisions
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{| class="wikitable" | {| class="wikitable" | ||
!Laboratory Method | !Laboratory Method | ||
!Findings | ! colspan="3" |Findings | ||
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|Microscopy | | rowspan="2" |Microscopy | ||
|Microscopic techniques include 1. Wet preparation 2. Concentration 3. Permanently stained smears<ref name="pmid14987356">{{cite journal| author=Huston CD, Haque R, Petri WA| title=Molecular-based diagnosis of Entamoeba histolytica infection. | journal=Expert Rev Mol Med | year= 1999 | volume= 1999 | issue= | pages= 1-11 | pmid=14987356 | doi=doi:10.1017/S1462399499000599 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=14987356 }} </ref> | | colspan="3" | | ||
'''Wet (Saline) preparation''' | * Microscopic techniques include 1. Wet preparation 2. Concentration 3. Permanently stained smears<ref name="pmid14987356">{{cite journal| author=Huston CD, Haque R, Petri WA| title=Molecular-based diagnosis of Entamoeba histolytica infection. | journal=Expert Rev Mol Med | year= 1999 | volume= 1999 | issue= | pages= 1-11 | pmid=14987356 | doi=doi:10.1017/S1462399499000599 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=14987356 }} </ref> | ||
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|'''Wet (Saline) preparation''' | |||
* Very insensitive method (<10%) Procedure | * Very insensitive method (<10%) Procedure | ||
* The sample is a fresh specimen that should be examined within 1 hr of collection | * The sample is a fresh specimen that should be examined within 1 hr of collection | ||
* The test is positive when RBCs in trophozoites are detected | * The test is positive when RBCs in trophozoites are detected | ||
* It is not used in patients without acute dysentery as trophozoites will not contain RBCs | * It is not used in patients without acute dysentery as trophozoites will not contain RBCs | ||
'''Concentration Technique''' | |'''Concentration Technique''' | ||
* It is helpful in detecting cysts in the stool sample in asymptomatic carriers | * It is helpful in detecting cysts in the stool sample in asymptomatic carriers | ||
'''Permanently stained smears''' | |'''Permanently stained smears''' | ||
* This is an important method for identification and recovery of Entamoeba species | * This is an important method for identification and recovery of Entamoeba species | ||
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|Culture Methods | |Culture Methods | ||
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|Isoenzyme analysis | |Isoenzyme analysis | ||
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|Antibody Detection Tests | |Antibody Detection Tests | ||
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|Antigen Detection Tests | |Antigen Detection Tests | ||
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|Immunochromatographic Assays | |Immunochromatographic Assays | ||
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Revision as of 00:00, 10 February 2017
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Amoebic liver abscess Microchapters |
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Diagnosis |
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Treatment |
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Case Studies |
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Amoebic liver abscess other diagnostic studies On the Web |
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American Roentgen Ray Society Images of Amoebic liver abscess other diagnostic studies |
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Risk calculators and risk factors for Amoebic liver abscess other diagnostic studies |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1];Associate Editor(s)-in-Chief: Yamuna Kondapally, M.B.B.S[2]
Overview
Other Diagnostic Tests
Needle aspiration
- Needle aspiration is used to differentiate between amoebic and pyogenic liver abscess
- The ultrasound and CT are used to guide percutaneous aspiration and drainage.
- The aspirated fluid is odorless unless secondarily infected
- The pathognomonic of the aspirate is the reddish-brown anchovy paste appearance, which indicates that the abscess has been present for weeks.
- The trophozoites are present only in the wall of the abscess. Rest of abscess is composed of lysed leukocytes.
| Laboratory Method | Findings | ||
|---|---|---|---|
| Microscopy |
| ||
Wet (Saline) preparation
|
Concentration Technique
|
Permanently stained smears
| |
| Culture Methods | |||
| Isoenzyme analysis | |||
| Antibody Detection Tests | |||
| Antigen Detection Tests | |||
| Immunochromatographic Assays | |||
DNA-Based Diagnostic Tests
| Laboratory Methods | Findings |
|---|---|
| Manual Methods | |
| Automated Methods | |
| Conventional PCR | |
| Real-Time PCR | |
| Microarray Development | |
| Typing Methods |
References
- ↑ Huston CD, Haque R, Petri WA (1999). "Molecular-based diagnosis of Entamoeba histolytica infection". Expert Rev Mol Med. 1999: 1–11. doi:doi:10.1017/S1462399499000599 Check
|doi=value (help). PMID 14987356.