Amoebic liver abscess other diagnostic studies: Difference between revisions
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* MtermiMtenMlyMinimum three stool samples are examined for ova and parasites within 10days of sample collection as organisms are excreted intermittently<ref name="pmid8863036">{{cite journal| author=Li E, Stanley SL| title=Protozoa. Amebiasis. | journal=Gastroenterol Clin North Am | year= 1996 | volume= 25 | issue= 3 | pages= 471-92 | pmid=8863036 | doi= | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=8863036 }} </ref> | * MtermiMtenMlyMinimum three stool samples are examined for ova and parasites within 10days of sample collection as organisms are excreted intermittently<ref name="pmid8863036">{{cite journal| author=Li E, Stanley SL| title=Protozoa. Amebiasis. | journal=Gastroenterol Clin North Am | year= 1996 | volume= 25 | issue= 3 | pages= 471-92 | pmid=8863036 | doi= | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=8863036 }} </ref> | ||
* Presence of RBCs in trophozoites is diagnostic of Entamoeba histolytica | * Presence of RBCs in trophozoites is diagnostic of Entamoeba histolytica | ||
* | * The specificity of the test is low as the trophozoites containing RBCs are not present in all cases and E dispar may also contain RBCs in trophozoites | ||
|- | |- | ||
|'''Wet (Saline) preparation''' | |'''Wet (Saline) preparation''' | ||
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1];Associate Editor(s)-in-Chief: Yamuna Kondapally, M.B.B.S[2]
Overview
Other Diagnostic Tests
Needle aspiration
- Needle aspiration is used to differentiate between amoebic and pyogenic liver abscess
- The ultrasound and CT are used to guide percutaneous aspiration and drainage.
- The aspirated fluid is odorless unless secondarily infected
- The pathognomonic of the aspirate is the reddish-brown anchovy paste appearance, which indicates that the abscess has been present for weeks.
- The trophozoites are present only in the wall of the abscess. Rest of abscess is composed of lysed leukocytes.
| Laboratory Method | Findings | ||
|---|---|---|---|
| Microscopy |
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Wet (Saline) preparation
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Concentration Technique
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Permanently stained smears
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| Culture Methods | |||
| Isoenzyme analysis | |||
| Antibody Detection Tests | |||
| Antigen Detection Tests | |||
| Immunochromatographic Assays | |||
DNA-Based Diagnostic Tests
| Laboratory Methods | Findings |
|---|---|
| Manual Methods | |
| Automated Methods | |
| Conventional PCR | |
| Real-Time PCR | |
| Microarray Development | |
| Typing Methods |
References
- ↑ Huston CD, Haque R, Petri WA (1999). "Molecular-based diagnosis of Entamoeba histolytica infection". Expert Rev Mol Med. 1999: 1–11. doi:doi:10.1017/S1462399499000599 Check
|doi=value (help). PMID 14987356. - ↑ Tanyuksel M, Petri WA (2003). "Laboratory diagnosis of amebiasis". Clin Microbiol Rev. 16 (4): 713–29. PMC 207118. PMID 14557296.
- ↑ Krogstad DJ, Spencer HC, Healy GR, Gleason NN, Sexton DJ, Herron CA (1978). "Amebiasis: epidemiologic studies in the United States, 1971-1974". Ann Intern Med. 88 (1): 89–97. PMID 619763.
- ↑ Li E, Stanley SL (1996). "Protozoa. Amebiasis". Gastroenterol Clin North Am. 25 (3): 471–92. PMID 8863036.