'''Immunohistochemical markers:'''<ref name="pmid29262668">{{cite journal |vauthors=Boccellino M, Vanacore D, Zappavigna S, Cavaliere C, Rossetti S, D'Aniello C, Chieffi P, Amler E, Buonerba C, Di Lorenzo G, Di Franco R, Izzo A, Piscitelli R, Iovane G, Muto P, Botti G, Perdonà S, Caraglia M, Facchini G |title=Testicular cancer from diagnosis to epigenetic factors |journal=Oncotarget |volume=8 |issue=61 |pages=104654–104663 |date=November 2017 |pmid=29262668 |pmc=5732834 |doi=10.18632/oncotarget.20992 |url=}}</ref>
Seminomas
Stain positively for:<ref name="pmid17527070">{{cite journal |vauthors=Santagata S, Ligon KL, Hornick JL |title=Embryonic stem cell transcription factor signatures in the diagnosis of primary and metastatic germ cell tumors |journal=Am. J. Surg. Pathol. |volume=31 |issue=6 |pages=836–45 |date=June 2007 |pmid=17527070 |doi=10.1097/PAS.0b013e31802e708a |url=}}</ref><ref name="pmid17117392">{{cite journal |vauthors=Cheng L, Sung MT, Cossu-Rocca P, Jones TD, MacLennan GT, De Jong J, Lopez-Beltran A, Montironi R, Looijenga LH |title=OCT4: biological functions and clinical applications as a marker of germ cell neoplasia |journal=J. Pathol. |volume=211 |issue=1 |pages=1–9 |date=January 2007 |pmid=17117392 |doi=10.1002/path.2105 |url=}}</ref>
*OCT3/4 transcription factors<ref name="pmid15818593">{{cite journal |vauthors=de Jong J, Stoop H, Dohle GR, Bangma CH, Kliffen M, van Esser JW, van den Bent M, Kros JM, Oosterhuis JW, Looijenga LH |title=Diagnostic value of OCT3/4 for pre-invasive and invasive testicular germ cell tumours |journal=J. Pathol. |volume=206 |issue=2 |pages=242–9 |date=June 2005 |pmid=15818593 |doi=10.1002/path.1766 |url=}}</ref>
*C-kit
*CD30
*D2-40 monoclonal antibody
*PLAP
*NANOG transcription factor
Embryonal carcinoma
Stain positively for:<ref name="pmid17527070">{{cite journal |vauthors=Santagata S, Ligon KL, Hornick JL |title=Embryonic stem cell transcription factor signatures in the diagnosis of primary and metastatic germ cell tumors |journal=Am. J. Surg. Pathol. |volume=31 |issue=6 |pages=836–45 |date=June 2007 |pmid=17527070 |doi=10.1097/PAS.0b013e31802e708a |url=}}</ref><ref name="pmid17117392">{{cite journal |vauthors=Cheng L, Sung MT, Cossu-Rocca P, Jones TD, MacLennan GT, De Jong J, Lopez-Beltran A, Montironi R, Looijenga LH |title=OCT4: biological functions and clinical applications as a marker of germ cell neoplasia |journal=J. Pathol. |volume=211 |issue=1 |pages=1–9 |date=January 2007 |pmid=17117392 |doi=10.1002/path.2105 |url=}}</ref>
*PLAP
*OCT3/4<ref name="pmid15818593">{{cite journal |vauthors=de Jong J, Stoop H, Dohle GR, Bangma CH, Kliffen M, van Esser JW, van den Bent M, Kros JM, Oosterhuis JW, Looijenga LH |title=Diagnostic value of OCT3/4 for pre-invasive and invasive testicular germ cell tumours |journal=J. Pathol. |volume=206 |issue=2 |pages=242–9 |date=June 2005 |pmid=15818593 |doi=10.1002/path.1766 |url=}}</ref>