PABPC4: Difference between revisions

Jump to navigation Jump to search
VisualWikitext
WikiBot (talk | contribs)
Bots, Bureaucrats, bureaucrats, interwiki, nocaptcha, Administrators
57,550 edits
m Robot: Automated text replacement (-{{WikiDoc Cardiology Network Infobox}} +, -<references /> +{{reflist|2}}, -{{reflist}} +{{reflist|2}})
 
m Bot: HTTP→HTTPS (v470)
 
Line 1: Line 1:
<!-- The PBB_Controls template provides controls for Protein Box Bot, please see Template:PBB_Controls for details. -->
{{Infobox_gene}}
{{PBB_Controls
'''Polyadenylate-binding protein 4''' ('''PABPC4''') is a [[protein]] that in humans is encoded by the ''PABPC4'' [[gene]].<ref name="pmid10543404">{{cite journal | vauthors = Féral C, Mattéi MG, Pawlak A, Guellaën G | title = Chromosomal localization of three human poly(A)-binding protein genes and four related pseudogenes | journal = Hum Genet | volume = 105 | issue = 4 | pages = 347–53 | date = Nov 1999 | pmid = 10543404 | pmc = 1865476 | doi = 10.1007/s004390051113 }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: PABPC4 poly(A) binding protein, cytoplasmic 4 (inducible form)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8761| accessdate = }}</ref>
| update_page = yes
| require_manual_inspection = no
| update_protein_box = yes
| update_summary = yes
| update_citations = yes
}}


<!-- The GNF_Protein_box is automatically maintained by Protein Box Bot.  See Template:PBB_Controls to Stop updates. -->
== Function ==
{{GNF_Protein_box
| image = PBB_Protein_PABPC4_image.jpg
| image_source = [[Protein_Data_Bank|PDB]] rendering based on 1cvj.
| PDB = {{PDB2|1cvj}}
| Name = Poly(A) binding protein, cytoplasmic 4 (inducible form)
| HGNCid = 8557
| Symbol = PABPC4
| AltSymbols =; APP-1; APP1; PABP4; iPABP
| OMIM = 603407
| ECnumber = 
| Homologene = 37855
| MGIid = 2385206
| GeneAtlas_image1 = PBB_GE_PABPC4_201064_s_at_tn.png
| Function = {{GNF_GO|id=GO:0000166 |text = nucleotide binding}} {{GNF_GO|id=GO:0003676 |text = nucleic acid binding}} {{GNF_GO|id=GO:0003723 |text = RNA binding}} {{GNF_GO|id=GO:0005515 |text = protein binding}} {{GNF_GO|id=GO:0008143 |text = poly(A) binding}} {{GNF_GO|id=GO:0008266 |text = poly(U) binding}} {{GNF_GO|id=GO:0017130 |text = poly(rC) binding}}
| Component = {{GNF_GO|id=GO:0005737 |text = cytoplasm}}
| Process = {{GNF_GO|id=GO:0006396 |text = RNA processing}} {{GNF_GO|id=GO:0006401 |text = RNA catabolic process}} {{GNF_GO|id=GO:0006412 |text = translation}} {{GNF_GO|id=GO:0007596 |text = blood coagulation}}
| Orthologs = {{GNF_Ortholog_box
    | Hs_EntrezGene = 8761
    | Hs_Ensembl = ENSG00000090621
    | Hs_RefseqProtein = NP_003810
    | Hs_RefseqmRNA = NM_003819
    | Hs_GenLoc_db = 
    | Hs_GenLoc_chr = 1
    | Hs_GenLoc_start = 39799075
    | Hs_GenLoc_end = 39815049
    | Hs_Uniprot = Q13310
    | Mm_EntrezGene = 230721
    | Mm_Ensembl = 
    | Mm_RefseqmRNA = NM_130881
    | Mm_RefseqProtein = NP_570951
    | Mm_GenLoc_db = 
    | Mm_GenLoc_chr = 
    | Mm_GenLoc_start = 
    | Mm_GenLoc_end = 
    | Mm_Uniprot = 
  }}
}}
'''Poly(A) binding protein, cytoplasmic 4 (inducible form)''', also known as '''PABPC4''', is a human [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: PABPC4 poly(A) binding protein, cytoplasmic 4 (inducible form)| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8761| accessdate = }}</ref>


<!-- The PBB_Summary template is automatically maintained by Protein Box Bot.  See Template:PBB_Controls to Stop updates. -->
Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.<ref name="entrez" />
{{PBB_Summary
| section_title =
| summary_text = Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.<ref name="entrez">{{cite web | title = Entrez Gene: PABPC4 poly(A) binding protein, cytoplasmic 4 (inducible form)| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8761| accessdate = }}</ref>
}}


==References==
== Model organisms ==
{{reflist|2}}
 
==Further reading==
{| class="wikitable sortable collapsible collapsed" border="1" cellpadding="2" style="float: right;" |
|+ ''Pabpc4'' knockout mouse phenotype
|-
! Characteristic!! Phenotype
 
|-
| [[Homozygote]] viability || bgcolor="#488ED3"|Normal
|-
| Fertility || bgcolor="#488ED3"|Normal
|-
| Body weight || bgcolor="#488ED3"|Normal
|-
| [[Open Field (animal test)|Anxiety]] || bgcolor="#488ED3"|Normal
|-
| Neurological assessment || bgcolor="#488ED3"|Normal
|-
| Grip strength || bgcolor="#488ED3"|Normal
|-
| [[Hot plate test|Hot plate]] || bgcolor="#488ED3"|Normal
|-
| [[Dysmorphology]] || bgcolor="#488ED3"|Normal
|-
| [[Indirect calorimetry]] || bgcolor="#488ED3"|Normal
|-
| [[Glucose tolerance test]] || bgcolor="#C40000"|Abnormal<ref name="Glucose tolerance test">{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MAUA/glucose-tolerance-ip/ |title=Glucose tolerance test data for Pabpc4 |publisher=Wellcome Trust Sanger Institute}}</ref>
|-
| [[Auditory brainstem response]] || bgcolor="#488ED3"|Normal
|-
| [[Dual-energy X-ray absorptiometry|DEXA]] || bgcolor="#488ED3"|Normal
|-
| [[Radiography]] || bgcolor="#488ED3"|Normal
|-
| Body temperature || bgcolor="#488ED3"|Normal
|-
| Eye morphology || bgcolor="#488ED3"|Normal
|-
| [[Clinical chemistry]] || bgcolor="#488ED3"|Normal
|-
| [[Haematology]] || bgcolor="#488ED3"|Normal
|-
| [[Peripheral blood lymphocyte]]s || bgcolor="#488ED3"|Normal
|-
| [[Micronucleus test]] || bgcolor="#488ED3"|Normal
|-
| Heart weight || bgcolor="#488ED3"|Normal
|-
| colspan=2; style="text-align: center;" | All tests and analysis from<ref name="mgp_reference">{{cite journal | doi = 10.1111/j.1755-3768.2010.4142.x | title = The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice | year = 2010 | last1 = Gerdin | first1 = AK | journal = Acta Ophthalmologica | volume = 88 | pages =  925–7 }}</ref><ref>[http://www.sanger.ac.uk/mouseportal/ Mouse Resources Portal], Wellcome Trust Sanger Institute.</ref>
|}
[[Model organism]]s have been used in the study of PABPC4 function. A conditional [[knockout mouse]] line, called ''Pabpc4<sup>tm1a(KOMP)Wtsi</sup>''<ref name="allele_ref">{{cite web |url=http://www.knockoutmouse.org/martsearch/search?query=Pabpc4 |title=International Knockout Mouse Consortium}}</ref><ref name="mgi_allele_ref">{{cite web |url=http://www.informatics.jax.org/searchtool/Search.do?query=MGI:4364054 |title=Mouse Genome Informatics}}</ref> was generated as part of the [[International Knockout Mouse Consortium]] program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.<ref name="pmid21677750">{{cite journal | vauthors = Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A | title = A conditional knockout resource for the genome-wide study of mouse gene function | journal = Nature | volume = 474 | issue = 7351 | pages = 337–342 | year = 2011 | pmid = 21677750 | pmc = 3572410 | doi = 10.1038/nature10163 }}</ref><ref name="mouse_library">{{cite journal | vauthors = Dolgin E | title = Mouse library set to be knockout | journal = Nature | volume = 474 | issue = 7351 | pages = 262–3 | year = 2011 | pmid = 21677718 | doi = 10.1038/474262a }}</ref><ref name="mouse_for_all_reasons">{{cite journal | vauthors = Collins FS, Rossant J, Wurst W | title = A Mouse for All Reasons | journal = Cell | volume = 128 | issue = 1 | pages = 9–13 | year = 2007 | pmid = 17218247 | doi = 10.1016/j.cell.2006.12.018 }}</ref>
 
Male and female animals underwent a standardized [[phenotypic screen]] to determine the effects of deletion.<ref name="mgp_reference" /><ref name="pmid21722353">{{cite journal | vauthors = van der Weyden L, White JK, Adams DJ, Logan DW | title = The mouse genetics toolkit: revealing function and mechanism. | journal = Genome Biol | volume = 12 | issue = 6 | pages = 224 | year = 2011 | pmid = 21722353 | pmc = 3218837 | doi = 10.1186/gb-2011-12-6-224 }}</ref> Twenty tests were carried out on [[mutant]] mice and one significant abnormality was observed: female homozygous mutants displayed [[impaired glucose tolerance]].<ref name="mgp_reference" />
 
== Interactions ==
 
PABPC4 has been shown to [[Protein-protein interaction|interact]] with [[PHLDA1]].<ref name=pmid11369516>{{cite journal | vauthors = Hinz T, Flindt S, Marx A, Janssen O, Kabelitz D | title = Inhibition of protein synthesis by the T cell receptor-inducible human TDAG51 gene product | journal = Cell. Signal. | volume = 13 | issue = 5 | pages = 345–52 | date = May 2001 | pmid = 11369516 | doi = 10.1016/S0898-6568(01)00141-3 }}</ref>
 
== References ==
{{reflist}}
 
== Further reading ==
{{refbegin | 2}}
{{refbegin | 2}}
{{PBB_Further_reading
* {{cite journal | vauthors = Maruyama K, Sugano S | title = Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides | journal = Gene | volume = 138 | issue = 1–2 | pages = 171–4 | year = 1994 | pmid = 8125298 | doi = 10.1016/0378-1119(94)90802-8 }}
| citations =
* {{cite journal | vauthors = Yang H, Duckett CS, Lindsten T | title = iPABP, an inducible poly(A)-binding protein detected in activated human T cells | journal = Mol. Cell. Biol. | volume = 15 | issue = 12 | pages = 6770–6 | year = 1996 | pmid = 8524242 | pmc = 230930 | doi =  }}
*{{cite journal | author=Maruyama K, Sugano S |title=Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. |journal=Gene |volume=138 |issue= 1-2 |pages= 171-4 |year= 1994 |pmid= 8125298 |doi= }}
* {{cite journal | vauthors = Houng AK, Maggini L, Clement CY, Reed GL | title = Identification and structure of activated-platelet protein-1, a protein with RNA-binding domain motifs that is expressed by activated platelets | journal = Eur. J. Biochem. | volume = 243 | issue = 1–2 | pages = 209–18 | year = 1997 | pmid = 9030741 | doi = 10.1111/j.1432-1033.1997.0209a.x }}
*{{cite journal | author=Yang H, Duckett CS, Lindsten T |title=iPABP, an inducible poly(A)-binding protein detected in activated human T cells. |journal=Mol. Cell. Biol. |volume=15 |issue= 12 |pages= 6770-6 |year= 1996 |pmid= 8524242 |doi=  }}
* {{cite journal | vauthors = Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S | title = Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library | journal = Gene | volume = 200 | issue = 1–2 | pages = 149–56 | year = 1997 | pmid = 9373149 | doi = 10.1016/S0378-1119(97)00411-3 }}
*{{cite journal | author=Houng AK, Maggini L, Clement CY, Reed GL |title=Identification and structure of activated-platelet protein-1, a protein with RNA-binding domain motifs that is expressed by activated platelets. |journal=Eur. J. Biochem. |volume=243 |issue= 1-2 |pages= 209-18 |year= 1997 |pmid= 9030741 |doi= }}
* {{cite journal | vauthors = Hoshino S, Imai M, Kobayashi T, Uchida N, Katada T | title = The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein | journal = J. Biol. Chem. | volume = 274 | issue = 24 | pages = 16677–80 | year = 1999 | pmid = 10358005 | doi = 10.1074/jbc.274.24.16677 }}
*{{cite journal | author=Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, ''et al.'' |title=Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. |journal=Gene |volume=200 |issue= 1-2 |pages= 149-56 |year= 1997 |pmid= 9373149 |doi= }}
* {{cite journal | vauthors = Hinz T, Flindt S, Marx A, Janssen O, Kabelitz D | title = Inhibition of protein synthesis by the T cell receptor-inducible human TDAG51 gene product | journal = Cell. Signal. | volume = 13 | issue = 5 | pages = 345–52 | year = 2001 | pmid = 11369516 | doi = 10.1016/S0898-6568(01)00141-3 }}
*{{cite journal | author=Hoshino S, Imai M, Kobayashi T, ''et al.'' |title=The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein. |journal=J. Biol. Chem. |volume=274 |issue= 24 |pages= 16677-80 |year= 1999 |pmid= 10358005 |doi= }}
* {{cite journal | vauthors = Li J, Hawkins IC, Harvey CD, Jennings JL, Link AJ, Patton JG | title = Regulation of Alternative Splicing by SRrp86 and Its Interacting Proteins | journal = Mol. Cell. Biol. | volume = 23 | issue = 21 | pages = 7437–47 | year = 2003 | pmid = 14559993 | pmc = 207616 | doi = 10.1128/MCB.23.21.7437-7447.2003 }}
*{{cite journal  | author=Féral C, Mattéi MG, Pawlak A, Guellaën G |title=Chromosomal localization of three human poly(A)-binding protein genes and four related pseudogenes. |journal=Hum. Genet. |volume=105 |issue= 4 |pages= 347-53 |year= 1999 |pmid= 10543404 |doi=  }}
* {{cite journal | vauthors = Lehner B, Sanderson CM | title = A Protein Interaction Framework for Human mRNA Degradation | journal = Genome Res. | volume = 14 | issue = 7 | pages = 1315–23 | year = 2004 | pmid = 15231747 | pmc = 442147 | doi = 10.1101/gr.2122004 }}
*{{cite journal | author=Hinz T, Flindt S, Marx A, ''et al.'' |title=Inhibition of protein synthesis by the T cell receptor-inducible human TDAG51 gene product. |journal=Cell. Signal. |volume=13 |issue= 5 |pages= 345-52 |year= 2001 |pmid= 11369516 |doi= }}
* {{cite journal | vauthors = Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM | title = Functional Proteomics Mapping of a Human Signaling Pathway | journal = Genome Res. | volume = 14 | issue = 7 | pages = 1324–32 | year = 2004 | pmid = 15231748 | pmc = 442148 | doi = 10.1101/gr.2334104 }}
*{{cite journal  | author=Strausberg RL, Feingold EA, Grouse LH, ''et al.'' |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899-903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 }}
* {{cite journal | vauthors = Goehler H, Lalowski M, Stelzl U, Waelter S, Stroedicke M, Worm U, Droege A, Lindenberg KS, Knoblich M, Haenig C, Herbst M, Suopanki J, Scherzinger E, Abraham C, Bauer B, Hasenbank R, Fritzsche A, Ludewig AH, Büssow K, Buessow K, Coleman SH, Gutekunst CA, Landwehrmeyer BG, Lehrach H, Wanker EE | title = A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease | journal = Mol. Cell | volume = 15 | issue = 6 | pages = 853–65 | year = 2004 | pmid = 15383276 | doi = 10.1016/j.molcel.2004.09.016 }}
*{{cite journal | author=Li J, Hawkins IC, Harvey CD, ''et al.'' |title=Regulation of alternative splicing by SRrp86 and its interacting proteins. |journal=Mol. Cell. Biol. |volume=23 |issue= 21 |pages= 7437-47 |year= 2003 |pmid= 14559993 |doi= }}
* {{cite journal | vauthors = Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ | title = Immunoaffinity profiling of tyrosine phosphorylation in cancer cells | journal = Nat. Biotechnol. | volume = 23 | issue = 1 | pages = 94–101 | year = 2005 | pmid = 15592455 | doi = 10.1038/nbt1046 }}
*{{cite journal  | author=Ota T, Suzuki Y, Nishikawa T, ''et al.'' |title=Complete sequencing and characterization of 21,243 full-length human cDNAs. |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40-5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 }}
* {{cite journal | vauthors = Ong SE, Mittler G, Mann M | title = Identifying and quantifying in vivo methylation sites by heavy methyl SILAC | journal = Nat. Methods | volume = 1 | issue = 2 | pages = 119–26 | year = 2005 | pmid = 15782174 | doi = 10.1038/nmeth715 }}
*{{cite journal | author=Lehner B, Sanderson CM |title=A protein interaction framework for human mRNA degradation. |journal=Genome Res. |volume=14 |issue= 7 |pages= 1315-23 |year= 2004 |pmid= 15231747 |doi= 10.1101/gr.2122004 }}
* {{cite journal | vauthors = Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D | title = Large-scale mapping of human protein–protein interactions by mass spectrometry | journal = Mol. Syst. Biol. | volume = 3 | issue = 1 | pages = 89 | year = 2007 | pmid = 17353931 | pmc = 1847948 | doi = 10.1038/msb4100134 }}
*{{cite journal | author=Colland F, Jacq X, Trouplin V, ''et al.'' |title=Functional proteomics mapping of a human signaling pathway. |journal=Genome Res. |volume=14 |issue= 7 |pages= 1324-32 |year= 2004 |pmid= 15231748 |doi= 10.1101/gr.2334104 }}
*{{cite journal | author=Goehler H, Lalowski M, Stelzl U, ''et al.'' |title=A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease. |journal=Mol. Cell |volume=15 |issue= 6 |pages= 853-65 |year= 2004 |pmid= 15383276 |doi= 10.1016/j.molcel.2004.09.016 }}
*{{cite journal | author=Gerhard DS, Wagner L, Feingold EA, ''et al.'' |title=The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121-7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 }}
*{{cite journal  | author=Rush J, Moritz A, Lee KA, ''et al.'' |title=Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. |journal=Nat. Biotechnol. |volume=23 |issue= 1 |pages= 94-101 |year= 2005 |pmid= 15592455 |doi= 10.1038/nbt1046 }}
*{{cite journal | author=Ong SE, Mittler G, Mann M |title=Identifying and quantifying in vivo methylation sites by heavy methyl SILAC. |journal=Nat. Methods |volume=1 |issue= 2 |pages= 119-26 |year= 2005 |pmid= 15782174 |doi= 10.1038/nmeth715 }}
*{{cite journal | author=Ewing RM, Chu P, Elisma F, ''et al.'' |title=Large-scale mapping of human protein-protein interactions by mass spectrometry. |journal=Mol. Syst. Biol. |volume=3 |issue= |pages= 89 |year= 2007 |pmid= 17353931 |doi= 10.1038/msb4100134 }}
}}
{{refend}}
{{refend}}


{{protein-stub}}
{{PDB Gallery|geneid=8761}}
{{WikiDoc Sources}}
{{RNA-binding proteins}}
 
[[Category:Genes mutated in mice]]

Latest revision as of 02:17, 27 October 2017

VALUE_ERROR (nil)
Identifiers
Aliases
External IDsGeneCards: [1]
Orthologs
SpeciesHumanMouse
Entrez

n/a

n/a

Ensembl

n/a

n/a

UniProt

n/a

n/a

RefSeq (mRNA)

n/a

n/a

RefSeq (protein)

n/a

n/a

Location (UCSC)n/an/a
PubMed searchn/an/a
Wikidata
View/Edit Human

Polyadenylate-binding protein 4 (PABPC4) is a protein that in humans is encoded by the PABPC4 gene.[1][2]

Function

Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.[2]

Model organisms

Model organisms have been used in the study of PABPC4 function. A conditional knockout mouse line, called Pabpc4tm1a(KOMP)Wtsi[6][7] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[8][9][10]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[4][11] Twenty tests were carried out on mutant mice and one significant abnormality was observed: female homozygous mutants displayed impaired glucose tolerance.[4]

Interactions

PABPC4 has been shown to interact with PHLDA1.[12]

References

  1. ↑ Féral C, Mattéi MG, Pawlak A, Guellaën G (Nov 1999). "Chromosomal localization of three human poly(A)-binding protein genes and four related pseudogenes". Hum Genet. 105 (4): 347–53. doi:10.1007/s004390051113. PMC 1865476. PMID 10543404.
  2. ↑ 2.0 2.1 "Entrez Gene: PABPC4 poly(A) binding protein, cytoplasmic 4 (inducible form)".
  3. ↑ "Glucose tolerance test data for Pabpc4". Wellcome Trust Sanger Institute.
  4. ↑ 4.0 4.1 4.2 Gerdin, AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
  5. ↑ Mouse Resources Portal, Wellcome Trust Sanger Institute.
  6. ↑ "International Knockout Mouse Consortium".
  7. ↑ "Mouse Genome Informatics".
  8. ↑ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
  9. ↑ Dolgin E (2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
  10. ↑ Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
  11. ↑ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
  12. ↑ Hinz T, Flindt S, Marx A, Janssen O, Kabelitz D (May 2001). "Inhibition of protein synthesis by the T cell receptor-inducible human TDAG51 gene product". Cell. Signal. 13 (5): 345–52. doi:10.1016/S0898-6568(01)00141-3. PMID 11369516.

Further reading

Retrieved from "https://www.wikidoc.org/index.php?title=PABPC4&oldid=1411208"