Sickle-cell disease laboratory findings: Difference between revisions
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{{Sickle-cell disease}} | {{Sickle-cell disease}} | ||
{{CMG}}; {{AE}} {{AN}} {{shyam}} | {{CMG}}; {{AE}} {{AN}}, {{shyam}} | ||
==Overview== | ==Overview== | ||
The most important laboratory test for sickle cell anemia is a complete blood count (CBC), specifically hemoglobin and hematocrit. | The most important laboratory test for sickle cell anemia is a complete blood count (CBC), specifically [[hemoglobin]] and [[hematocrit]]. | ||
==Laboratory findings== | ==Laboratory findings== | ||
===Blood tests=== | ===Blood tests=== | ||
Complete blood count (CBC) will reveal [[haemoglobin]] levels in the range of 6-8 g/dL with a high reticulocyte count (greater than 1.5%). | Complete blood count (CBC) will reveal [[haemoglobin]] levels in the range of 6-8 g/dL with a high [[reticulocyte count]] (greater than 1.5%). | ||
On a peripheral blood film, one can observe features of [[hyposplenism]] i.e., target cells and Howell-Jolly bodies. | On a peripheral blood film, one can observe features of [[hyposplenism]] i.e., target cells and Howell-Jolly bodies. | ||
Sickling of the red blood cells, on a blood film, can be induced by the addition of sodium metabisulphite. | Sickling of the red blood cells, on a blood film, can be induced by the addition of sodium metabisulphite. | ||
Another test is Sickle Solubility | Another test is Sickle Solubility Test. A mixture of haemoglobin S (Hb S) in a reducing solution e.g., sodium dithionite gives a turbid appearance while normal Hb gives a clear solution. | ||
Abnormal [[hemoglobin]] forms can be detected on [[hemoglobin electrophoresis]], a form of [[gel electrophoresis]] on which the various types of hemoglobin move at varying speed. Sickle-cell hemoglobin (HgbS) and [[hemoglobin C]] with sickling (HgbSC) | Abnormal [[hemoglobin]] forms can be detected on [[hemoglobin electrophoresis]], a form of [[gel electrophoresis]] on which the various types of hemoglobin move at varying speed. Sickle-cell hemoglobin (HgbS) and [[hemoglobin C]] with sickling (HgbSC) are the two most common forms and are identifiable. [[Genetic testing]] is rarely performed. | ||
For patients with sickle cell disease who present with possible infection, important laboratory testing may include: urinalysis, blood cultures, sputum cultures, and cerebrospinal fluid analysis. | For patients with sickle cell disease who present with possible infection, important laboratory testing may include: urinalysis, blood cultures, sputum cultures, and cerebrospinal fluid analysis. | ||
Revision as of 18:13, 24 October 2016
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Aarti Narayan, M.B.B.S [2], Shyam Patel [3]
Overview
The most important laboratory test for sickle cell anemia is a complete blood count (CBC), specifically hemoglobin and hematocrit.
Laboratory findings
Blood tests
Complete blood count (CBC) will reveal haemoglobin levels in the range of 6-8 g/dL with a high reticulocyte count (greater than 1.5%). On a peripheral blood film, one can observe features of hyposplenism i.e., target cells and Howell-Jolly bodies. Sickling of the red blood cells, on a blood film, can be induced by the addition of sodium metabisulphite. Another test is Sickle Solubility Test. A mixture of haemoglobin S (Hb S) in a reducing solution e.g., sodium dithionite gives a turbid appearance while normal Hb gives a clear solution. Abnormal hemoglobin forms can be detected on hemoglobin electrophoresis, a form of gel electrophoresis on which the various types of hemoglobin move at varying speed. Sickle-cell hemoglobin (HgbS) and hemoglobin C with sickling (HgbSC) are the two most common forms and are identifiable. Genetic testing is rarely performed.
For patients with sickle cell disease who present with possible infection, important laboratory testing may include: urinalysis, blood cultures, sputum cultures, and cerebrospinal fluid analysis.