Amoebic liver abscess other diagnostic studies

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1];Associate Editor(s)-in-Chief: Yamuna Kondapally, M.B.B.S[2]

Other Diagnostic Tests

Needle aspiration

• Needle aspiration is used to differentiate between amoebic and pyogenic liver abscess
• The ultrasound and CT are used to guide percutaneous aspiration and drainage.
• The aspirated fluid is odorless unless secondarily infected
• The pathognomonic of the aspirate is the reddish-brown anchovy paste appearance, which indicates that the abscess has been present for weeks.
• The trophozoites are present only in the wall of the abscess. Rest of abscess is composed of lysed leukocytes.

Laboratory Method	Findings
Microscopy	Microscopic techniques include: 1. Wet preparation 2. Concentration 3. Permanantly stained smears
Culture Methods
Isoenzyme analysis
Antibody Detection Tests
Antigen Detection Tests
Immunochromatographic Assays

DNA-Based Diagnostic Tests

Laboratory Methods	Findings
Manual Methods
Automated Methods
Conventional PCR
Real-Time PCR
Microarray Development
Typing Methods