Amoebic liver abscess other diagnostic studies: Difference between revisions
Jump to navigation
Jump to search
| Line 22: | Line 22: | ||
* Microscopic techniques include 1. Wet preparation 2. Concentration 3. Permanently stained smears<ref name="pmid14987356">{{cite journal| author=Huston CD, Haque R, Petri WA| title=Molecular-based diagnosis of Entamoeba histolytica infection. | journal=Expert Rev Mol Med | year= 1999 | volume= 1999 | issue= | pages= 1-11 | pmid=14987356 | doi=doi:10.1017/S1462399499000599 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=14987356 }} </ref> | * Microscopic techniques include 1. Wet preparation 2. Concentration 3. Permanently stained smears<ref name="pmid14987356">{{cite journal| author=Huston CD, Haque R, Petri WA| title=Molecular-based diagnosis of Entamoeba histolytica infection. | journal=Expert Rev Mol Med | year= 1999 | volume= 1999 | issue= | pages= 1-11 | pmid=14987356 | doi=doi:10.1017/S1462399499000599 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=14987356 }} </ref> | ||
* Less reliable method than culture or antigen detection test<ref name="pmid14557296">{{cite journal| author=Tanyuksel M, Petri WA| title=Laboratory diagnosis of amebiasis. | journal=Clin Microbiol Rev | year= 2003 | volume= 16 | issue= 4 | pages= 713-29 | pmid=14557296 | doi= | pmc=207118 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=14557296 }} </ref><ref name="pmid619763">{{cite journal| author=Krogstad DJ, Spencer HC, Healy GR, Gleason NN, Sexton DJ, Herron CA| title=Amebiasis: epidemiologic studies in the United States, 1971-1974. | journal=Ann Intern Med | year= 1978 | volume= 88 | issue= 1 | pages= 89-97 | pmid=619763 | doi= | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=619763 }} </ref> | * Less reliable method than culture or antigen detection test<ref name="pmid14557296">{{cite journal| author=Tanyuksel M, Petri WA| title=Laboratory diagnosis of amebiasis. | journal=Clin Microbiol Rev | year= 2003 | volume= 16 | issue= 4 | pages= 713-29 | pmid=14557296 | doi= | pmc=207118 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=14557296 }} </ref><ref name="pmid619763">{{cite journal| author=Krogstad DJ, Spencer HC, Healy GR, Gleason NN, Sexton DJ, Herron CA| title=Amebiasis: epidemiologic studies in the United States, 1971-1974. | journal=Ann Intern Med | year= 1978 | volume= 88 | issue= 1 | pages= 89-97 | pmid=619763 | doi= | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=619763 }} </ref> | ||
* Minimum three stool samples are examined for ova and parasites within 10 days of sample collection as organisms are excreted intermittently. | |||
* Presence of RBCs in trophozoites is diagnosticof Entamoeb his olytica | |||
|- | |- | ||
|'''Wet (Saline) preparation''' | |'''Wet (Saline) preparation''' | ||
Revision as of 00:44, 10 February 2017
|
Amoebic liver abscess Microchapters |
|
Diagnosis |
|
Treatment |
|
Case Studies |
|
Amoebic liver abscess other diagnostic studies On the Web |
|
American Roentgen Ray Society Images of Amoebic liver abscess other diagnostic studies |
|
Risk calculators and risk factors for Amoebic liver abscess other diagnostic studies |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1];Associate Editor(s)-in-Chief: Yamuna Kondapally, M.B.B.S[2]
Overview
Other Diagnostic Tests
Needle aspiration
- Needle aspiration is used to differentiate between amoebic and pyogenic liver abscess
- The ultrasound and CT are used to guide percutaneous aspiration and drainage.
- The aspirated fluid is odorless unless secondarily infected
- The pathognomonic of the aspirate is the reddish-brown anchovy paste appearance, which indicates that the abscess has been present for weeks.
- The trophozoites are present only in the wall of the abscess. Rest of abscess is composed of lysed leukocytes.
| Laboratory Method | Findings | ||
|---|---|---|---|
| Microscopy |
| ||
Wet (Saline) preparation
|
Concentration Technique
|
Permanently stained smears
| |
| Culture Methods | |||
| Isoenzyme analysis | |||
| Antibody Detection Tests | |||
| Antigen Detection Tests | |||
| Immunochromatographic Assays | |||
DNA-Based Diagnostic Tests
| Laboratory Methods | Findings |
|---|---|
| Manual Methods | |
| Automated Methods | |
| Conventional PCR | |
| Real-Time PCR | |
| Microarray Development | |
| Typing Methods |
References
- ↑ Huston CD, Haque R, Petri WA (1999). "Molecular-based diagnosis of Entamoeba histolytica infection". Expert Rev Mol Med. 1999: 1–11. doi:doi:10.1017/S1462399499000599 Check
|doi=value (help). PMID 14987356. - ↑ Tanyuksel M, Petri WA (2003). "Laboratory diagnosis of amebiasis". Clin Microbiol Rev. 16 (4): 713–29. PMC 207118. PMID 14557296.
- ↑ Krogstad DJ, Spencer HC, Healy GR, Gleason NN, Sexton DJ, Herron CA (1978). "Amebiasis: epidemiologic studies in the United States, 1971-1974". Ann Intern Med. 88 (1): 89–97. PMID 619763.