Amoebic liver abscess other diagnostic studies: Difference between revisions
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* The specimens for the culture of E histolytica include fecal specimens, rectal biopsy specimens or liver abscess aspirates. | * The specimens for the culture of E histolytica include fecal specimens, rectal biopsy specimens or liver abscess aspirates. | ||
* There are two culture techniques. They are xenic and axenic systems.<ref name="pmid12097242">{{cite journal| author=Clark CG, Diamond LS| title=Methods for cultivation of luminal parasitic protists of clinical importance. | journal=Clin Microbiol Rev | year= 2002 | volume= 15 | issue= 3 | pages= 329-41 | pmid=12097242 | doi= | pmc=118080 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12097242 }} </ref> | |||
*Culture of E histolytica is less sensitive than microscopy as a detection method. | |||
*Parasite cultures are expensive, labor intensive and difficult to maintain in the diagnostic laboratory. | |||
*Culture is not recommended as the routine diagnostic test due to overgrowth of other organisms like bacteria and fungi in the culture media. | |||
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|Isoenzyme analysis | |Isoenzyme analysis | ||
Revision as of 01:41, 10 February 2017
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1];Associate Editor(s)-in-Chief: Yamuna Kondapally, M.B.B.S[2]
Overview
Other Diagnostic Tests
Needle aspiration
- Needle aspiration is used to differentiate between amoebic and pyogenic liver abscess
- The ultrasound and CT are used to guide percutaneous aspiration and drainage.
- The aspirated fluid is odorless unless secondarily infected
- The pathognomonic of the aspirate is the reddish-brown anchovy paste appearance, which indicates that the abscess has been present for weeks.
- The trophozoites are present only in the wall of the abscess. Rest of abscess is composed of lysed leukocytes.
| Laboratory Method | Findings | ||
|---|---|---|---|
| Microscopy |
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Wet (Saline) preparation
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Concentration Technique
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Permanently stained smears
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| Culture Methods |
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| Isoenzyme analysis | |||
| Antibody Detection Tests | |||
| Antigen Detection Tests | |||
| Immunochromatographic Assays | |||
DNA-Based Diagnostic Tests
| Laboratory Methods | Findings |
|---|---|
| Manual Methods | |
| Automated Methods | |
| Conventional PCR | |
| Real-Time PCR | |
| Microarray Development | |
| Typing Methods |
References
- ↑ Huston CD, Haque R, Petri WA (1999). "Molecular-based diagnosis of Entamoeba histolytica infection". Expert Rev Mol Med. 1999: 1–11. doi:doi:10.1017/S1462399499000599 Check
|doi=value (help). PMID 14987356. - ↑ Tanyuksel M, Petri WA (2003). "Laboratory diagnosis of amebiasis". Clin Microbiol Rev. 16 (4): 713–29. PMC 207118. PMID 14557296.
- ↑ Krogstad DJ, Spencer HC, Healy GR, Gleason NN, Sexton DJ, Herron CA (1978). "Amebiasis: epidemiologic studies in the United States, 1971-1974". Ann Intern Med. 88 (1): 89–97. PMID 619763.
- ↑ Li E, Stanley SL (1996). "Protozoa. Amebiasis". Gastroenterol Clin North Am. 25 (3): 471–92. PMID 8863036.
- ↑ González-Ruiz A, Haque R, Aguirre A, Castañón G, Hall A, Guhl F; et al. (1994). "Value of microscopy in the diagnosis of dysentery associated with invasive Entamoeba histolytica". J Clin Pathol. 47 (3): 236–9. PMC 501902. PMID 8163695.
- ↑ Strachan WD, Chiodini PL, Spice WM, Moody AH, Ackers JP (1988). "Immunological differentiation of pathogenic and non-pathogenic isolates of Entamoeba histolytica". Lancet. 1 (8585): 561–3. PMID 2894495.
- ↑ Clark CG, Diamond LS (2002). "Methods for cultivation of luminal parasitic protists of clinical importance". Clin Microbiol Rev. 15 (3): 329–41. PMC 118080. PMID 12097242.