Enterobiasis laboratory findings: Difference between revisions
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The diagnostic characteristics of egg are: size 50-60 µm by 20-32 µm; typical elongated shape, with one convex side and one flattened side and colorless shell.<ref>{{cite web | url = http://pathmicro.med.sc.edu/parasitology/nematodes.htm| title =PARASITOLOGY - CHAPTER FOUR NEMATODES (Round Worms) | accessdate = 2007-10-18| publisher = The Board of Trustees of the University of South Carolina}}</ref><ref name="pmid21286054">{{cite journal |vauthors=Caldwell JP |title=Pinworms (enterobius vermicularis) |journal=Can Fam Physician |volume=28 |issue= |pages=306–9 |year=1982 |pmid=21286054 |pmc=2306321 |doi= |url=}}</ref> | The diagnostic characteristics of egg are: size 50-60 µm by 20-32 µm; typical elongated shape, with one convex side and one flattened side and colorless shell.<ref>{{cite web | url = http://pathmicro.med.sc.edu/parasitology/nematodes.htm| title =PARASITOLOGY - CHAPTER FOUR NEMATODES (Round Worms) | accessdate = 2007-10-18| publisher = The Board of Trustees of the University of South Carolina}}</ref><ref name="pmid21286054">{{cite journal |vauthors=Caldwell JP |title=Pinworms (enterobius vermicularis) |journal=Can Fam Physician |volume=28 |issue= |pages=306–9 |year=1982 |pmid=21286054 |pmc=2306321 |doi= |url=}}</ref> | ||
===Stool analysis=== | ===Stool analysis=== | ||
Stool analysis for ova and parasites is of low diagnostic yield. The actual worms may be seen in the host's feces; however the eggs are invisible to the naked eye. | Stool analysis for ova and parasites is of low diagnostic yield. The actual worms may be seen in the host's feces; however the eggs are invisible to the naked eye.<ref>{{cite web | url = http://pathmicro.med.sc.edu/parasitology/nematodes.htm| title =PARASITOLOGY - CHAPTER FOUR NEMATODES (Round Worms) | accessdate = 2007-10-18| publisher = The Board of Trustees of the University of South Carolina}}</ref><ref name="pmid21286054">{{cite journal |vauthors=Caldwell JP |title=Pinworms (enterobius vermicularis) |journal=Can Fam Physician |volume=28 |issue= |pages=306–9 |year=1982 |pmid=21286054 |pmc=2306321 |doi= |url=}}</ref> | ||
===Histology=== | ===Histology=== | ||
On [[histology|histologic]] cross-section, alae or wings (running the length of the worm) are identifying features of the pinworm (see micrograph).<ref>Diagnostic Findings Enterobiasis. [[Centers for Disease Control and Prevention]]. URL:[http://www.dpd.cdc.gov/dpdx/HTML/Enterobiasis.htm http://www.dpd.cdc.gov/dpdx/HTML/Enterobiasis.htm]. Accessed on: August 6, 2008.</ref> | On [[histology|histologic]] cross-section, alae or wings (running the length of the worm) are identifying features of the pinworm (see micrograph).<ref>Diagnostic Findings Enterobiasis. [[Centers for Disease Control and Prevention]]. URL:[http://www.dpd.cdc.gov/dpdx/HTML/Enterobiasis.htm http://www.dpd.cdc.gov/dpdx/HTML/Enterobiasis.htm]. Accessed on: August 6, 2008.</ref> |
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
Overview
Diagnosis is often made clinically by observing the female worm(s) in the peri-anal region, but can also be made using the "scotch-tape" test, in which the sticky side of a strip of cellophane tape is pressed against the peri-anal skin, then examined under a microscope for pinworm eggs.
Laboratory Findings
Scotch Tape Test
It is also called Hall or National Institute of Health swab. Sticky side of a strip of cellophane tape is pressed against the peri-anal skin, then examined under a microscope for pinworm eggs. The test is repeated for five consecutive mornings to increase the sensitivity to 99%. It is done prior to washing or defecation. The diagnostic characteristics of egg are: size 50-60 µm by 20-32 µm; typical elongated shape, with one convex side and one flattened side and colorless shell.[1][2]
Stool analysis
Stool analysis for ova and parasites is of low diagnostic yield. The actual worms may be seen in the host's feces; however the eggs are invisible to the naked eye.[3][2]
Histology
On histologic cross-section, alae or wings (running the length of the worm) are identifying features of the pinworm (see micrograph).[4]
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Two pinworms, captured on emergence from the anus. Markings are 1 mm apart.
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Pinworms are sometimes diagnosed incidentally by pathology. Micrograph of pinworms in the appendix. H&E stain.
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High magnification micrograph of a pinworm in cross-section in the appendix. H&E stain.
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Enterobius vermicularis egg under a light microscope.
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Pinworm eggs are easily seen under a microscope.
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Pinworms are sometimes diagnosed incidentally by pathology. Micrograph of male pinworm in cross-section. Alae (blue arrow), intestine (red arrow) and testis (black arrow). H&E stain.
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A pinworm (Enterobius vermicularis).
Videos Showing Pinworm Egg Under Microscope
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References
- ↑ "PARASITOLOGY - CHAPTER FOUR NEMATODES (Round Worms)". The Board of Trustees of the University of South Carolina. Retrieved 2007-10-18.
- ↑ 2.0 2.1 Caldwell JP (1982). "Pinworms (enterobius vermicularis)". Can Fam Physician. 28: 306–9. PMC 2306321. PMID 21286054.
- ↑ "PARASITOLOGY - CHAPTER FOUR NEMATODES (Round Worms)". The Board of Trustees of the University of South Carolina. Retrieved 2007-10-18.
- ↑ Diagnostic Findings Enterobiasis. Centers for Disease Control and Prevention. URL:http://www.dpd.cdc.gov/dpdx/HTML/Enterobiasis.htm. Accessed on: August 6, 2008.