Salmonella

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: João André Alves Silva, M.D. [2] Jolanta Marszalek, M.D. [3]

Overview

Salmonella is a genus of rod-shaped Gram-negative enterobacteria.^[1] Salmonella species are motile and produce hydrogen sulfide.^[2]

Taxonomy

Cellular organism; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae^[3]

Biology

Computer-generated image of three drug-resistant non-typhoidal Salmonella bacteria. *Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.*^[4]

Colonial growth pattern displayed by Salmonella enterica subsp. arizonae, formerly Salmonella choleraesuis and Arizona hinshawii bacteria grown on a blood agar culture plate *Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.*^[4]

Salmonella is a Gram-negative bacterium, facultatively intracellular anaerobic, non-spore-forming bacilli. It measures 2 to 3 by 0.4 to 0.6 μm. Salmonellae do not ferment lactose, reduce nitrates, produce acid on glucose fermentation and are non producers of cytochrome oxidase.^[5] Due to the presence of flagella, almost all salmonella are motile. 1% of the bacteria are able to ferment lactose, which may be responsible for its non-detection in media other than MacConkey agar.

For the isolation of salmonella in culture media, freshly passed stool are preferred. Common media for the growth of salmonella include: MacConkey agar, deoxycholate agar, and xylose-lysine-deoxycholate agar.^[6]

When the sample has a low number of bacteria, special enrichment broths, such as the selenite-based enrichment broth, may be used to increase the number of bacteria.^[6]

Infectious Cycle

Salmonella enterica enters the body through the mouth, by ingestion of contaminated food and water. For the bacteria to cause disease, an inoculum of about 50 000 bacteria is often required. Once in the intestine, the bacteria will first localize at the apical epithelium. Salmonella will then initiate bacterial mechanisms that allow host cell invasion, inducing inflammatory changes, such as:^[7]^[8]^[9]^[10]

Diffuse and focal infiltration of PMN
Crypt abscess
Necrosis of the epithelium
Fluid secretion
Edema

Different serovars will have different preferable intestinal locations. An example is the enterocolitis at the terminal ileum, cecum, and proximal colon caused by serovar Typhimurium. Intestinal disease is marked by neutrophil migration to the intestinal epithelium. This recruitment is done by the secretion of interleukin-8, induced by Salmonella.^[11]

Classification

Before 1983 Salmonella were classified in different species. However, genome studies have shown high levels of DNA similarities among the different types of salmonella, which lead to the actual classification of salmonella in 2 different species:

Salmonella enterica

Contains six subspecies - I, II, IIIa, IIIb, IV, and VI:^[12]^[13]
- I - enterica
- II - salamae
- III - arizonae
- IIIb - diarizonae
- IV - houtenae
- VI - indica
Subspecies I contains most pathogenic serotypes for humans (99.5%)
Subspecies IIIa and IIIb, formerly belonging to the genus Arizonae, are responsible for rare human infections

Salmonella bongori

Formerly subspecies V^[14]

Serovars

Salmonella subspecies may be serogrouped, into more than 2500 serovars with polyvalent antisera. For this division the following bacterial structures are considered:^[15]

Capsular antigen
Polysaccharide O antigens
Flagellar antigens

Different salmonella serotypes may also be distinguished in cultures according to different metabolism of sugars.^[16]

Tropism

In vitro Salmonella are able to interact with different cell types. However, in vivo, the bacteria was found to enter only certain human cells, namely:

Epithelial cells
Macrophages
Dendritic cells
Neutrophils

Natural Reservoir

Different salmonella serovars may have different natural reservoirs. Common types of serovars of salmonella enterica that infect the human gastrointestinal tract include the serovars Sendai, Typhi, and Paratyphi. These have humans as their only natural host.

Related Chapters

References

↑ Ryan KJ, Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed. ed.). McGraw Hill. ISBN 0-8385-8529-9.
↑ Giannella RA (1996). "Salmonella". In Baron S et al (eds.). Baron's Medical Microbiology (4th ed. ed.). Univ of Texas Medical Branch. ISBN 0-9631172-1-1.
↑ "Salmonella (Taxonomy)".
↑ ^4.0 ^4.1 "Public Health Image Library (PHIL), Centers for Disease Control and Prevention".
↑ Mandell, Gerald (2010). Mandell, Douglas, and Bennett's principles and practice of infectious diseases. Philadelphia, PA: Churchill Livingstone/Elsevier. ISBN 0443068399.
↑ ^6.0 ^6.1 Perez, J. M.; Cavalli, P.; Roure, C.; Renac, R.; Gille, Y.; Freydiere, A. M. (2003). "Comparison of Four Chromogenic Media and Hektoen Agar for Detection and Presumptive Identification of Salmonella Strains in Human Stools". Journal of Clinical Microbiology. 41 (3): 1130–1134. doi:10.1128/JCM.41.3.1130-1134.2003. ISSN 0095-1137.
↑ McGovern VJ, Slavutin LJ (1979). "Pathology of salmonella colitis". Am J Surg Pathol. 3 (6): 483–90. PMID 534385.
↑ Giannella RA, Formal SB, Dammin GJ, Collins H (1973). "Pathogenesis of salmonellosis. Studies of fluid secretion, mucosal invasion, and morphologic reaction in the rabbit ileum". J Clin Invest. 52 (2): 441–53. doi:10.1172/JCI107201. PMC 302274. PMID 4630603.
↑ Clarke RC, Gyles CL (1987). "Virulence of wild and mutant strains of Salmonella typhimurium in ligated intestinal segments of calves, pigs, and rabbits". Am J Vet Res. 48 (3): 504–10. PMID 3551701.
↑ Finlay BB, Heffron F, Falkow S (1989). "Epithelial cell surfaces induce Salmonella proteins required for bacterial adherence and invasion". Science. 243 (4893): 940–3. PMID 2919285.
↑ McCormick BA, Colgan SP, Delp-Archer C, Miller SI, Madara JL (1993). "Salmonella typhimurium attachment to human intestinal epithelial monolayers: transcellular signalling to subepithelial neutrophils". J Cell Biol. 123 (4): 895–907. PMC 2200157. PMID 8227148.
↑ "The type species of the genus Salmonella Lignieres 1900 is Salmonella enterica (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987, with the type strain LT2T, and conservation of the epithet enterica in Salmonella enterica over all earlier epithets that may be applied to this species. Opinion 80". Int J Syst Evol Microbiol. 55 (Pt 1): 519–20. 2005. PMID 15653929.
↑ Tindall BJ; Grimont PAD, Garrity GM; Euzéby JP (2005). "Nomenclature and taxonomy of the genus Salmonella". Int J Syst Evol Microbiol. 55: 521&ndash, 524. PMID 15653930.
↑ Popoff MY, Bockemühl J, Gheesling LL (2004). "Supplement 2002 (no. 46) to the Kauffmann-White scheme". Res Microbiol. 155 (7): 568–70. doi:10.1016/j.resmic.2004.04.005. PMID 15313257.
↑ Murray, Patrick (2013). Medical microbiology. Philadelphia: Elsevier/Saunders. ISBN 0323086926.
↑ Mandell, Gerald (2010). Mandell, Douglas, and Bennett's principles and practice of infectious diseases. Philadelphia, PA: Churchill Livingstone/Elsevier. ISBN 0443068399.

Template:WikiDoc Sources

[Sherris-1] Ryan KJ, Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed. ed.). McGraw Hill. ISBN 0-8385-8529-9.

[Baron-2] Giannella RA (1996). "Salmonella". In Baron S et al (eds.). Baron's Medical Microbiology (4th ed. ed.). Univ of Texas Medical Branch. ISBN 0-9631172-1-1.

[NCBI-3] "Salmonella (Taxonomy)".

[PHIL-4] 4.0 ^4.1 "Public Health Image Library (PHIL), Centers for Disease Control and Prevention".

[5] Mandell, Gerald (2010). Mandell, Douglas, and Bennett's principles and practice of infectious diseases. Philadelphia, PA: Churchill Livingstone/Elsevier. ISBN 0443068399.

[PerezCavalli2003-6] 6.0 ^6.1 Perez, J. M.; Cavalli, P.; Roure, C.; Renac, R.; Gille, Y.; Freydiere, A. M. (2003). "Comparison of Four Chromogenic Media and Hektoen Agar for Detection and Presumptive Identification of Salmonella Strains in Human Stools". Journal of Clinical Microbiology. 41 (3): 1130–1134. doi:10.1128/JCM.41.3.1130-1134.2003. ISSN 0095-1137.

[pmid534385-7] McGovern VJ, Slavutin LJ (1979). "Pathology of salmonella colitis". Am J Surg Pathol. 3 (6): 483–90. PMID 534385.

[pmid4630603-8] Giannella RA, Formal SB, Dammin GJ, Collins H (1973). "Pathogenesis of salmonellosis. Studies of fluid secretion, mucosal invasion, and morphologic reaction in the rabbit ileum". J Clin Invest. 52 (2): 441–53. doi:10.1172/JCI107201. PMC 302274. PMID 4630603.

[pmid3551701-9] Clarke RC, Gyles CL (1987). "Virulence of wild and mutant strains of Salmonella typhimurium in ligated intestinal segments of calves, pigs, and rabbits". Am J Vet Res. 48 (3): 504–10. PMID 3551701.

[pmid2919285-10] Finlay BB, Heffron F, Falkow S (1989). "Epithelial cell surfaces induce Salmonella proteins required for bacterial adherence and invasion". Science. 243 (4893): 940–3. PMID 2919285.

[pmid8227148-11] McCormick BA, Colgan SP, Delp-Archer C, Miller SI, Madara JL (1993). "Salmonella typhimurium attachment to human intestinal epithelial monolayers: transcellular signalling to subepithelial neutrophils". J Cell Biol. 123 (4): 895–907. PMC 2200157. PMID 8227148.

[Opinion80_2005-12] "The type species of the genus Salmonella Lignieres 1900 is Salmonella enterica (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987, with the type strain LT2T, and conservation of the epithet enterica in Salmonella enterica over all earlier epithets that may be applied to this species. Opinion 80". Int J Syst Evol Microbiol. 55 (Pt 1): 519–20. 2005. PMID 15653929.

[Tindall_2005-13] Tindall BJ; Grimont PAD, Garrity GM; Euzéby JP (2005). "Nomenclature and taxonomy of the genus Salmonella". Int J Syst Evol Microbiol. 55: 521&ndash, 524. PMID 15653930.

[pmid15313257-14] Popoff MY, Bockemühl J, Gheesling LL (2004). "Supplement 2002 (no. 46) to the Kauffmann-White scheme". Res Microbiol. 155 (7): 568–70. doi:10.1016/j.resmic.2004.04.005. PMID 15313257.

[15] Murray, Patrick (2013). Medical microbiology. Philadelphia: Elsevier/Saunders. ISBN 0323086926.

[16] Mandell, Gerald (2010). Mandell, Douglas, and Bennett's principles and practice of infectious diseases. Philadelphia, PA: Churchill Livingstone/Elsevier. ISBN 0443068399.

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