Cyclophilin

Revision as of 17:14, 7 September 2016 by en>Koifishkid (Removed the link to the cyclophilin/PPID page. These proteins are confusingly named. The protein described in this article is encoded by PPIF, not PPID. PPIF is part of the mitochondrial permeability transition pore, while PPID is not. ~~~~)
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peptidylprolyl isomerase A (cyclophilin A)
File:Cyclophilin A-cyclosporin complex 1CWA.png
Ribbon diagram of cyclophilin A in complex with ciclosporin (yellow). From PDB: 1CWA​.
Identifiers
SymbolPPIA
Entrez5478
HUGO9253
OMIM123840
RefSeqNM_203430
UniProtQ3KQW3
Other data
EC number5.2.1.8
LocusChr. 7 p13
Pro_isomerase
File:PDB 1w74 EBI.jpg
x-ray structure of peptidyl-prolyl cis-trans isomerase a, ppia, rv0009, from mycobacterium tuberculosis.
Identifiers
SymbolPro_isomerase
PfamPF00160
Pfam clanCL0475
InterProIPR002130
PROSITEPDOC00154
SCOP1cyh
SUPERFAMILY1cyh

Cyclophilins are a family of proteins from vertebrates and other organisms that bind to ciclosporin (cyclosporin A), an immunosuppressant which is usually used to suppress rejection after internal organ transplants.[1] These proteins have peptidyl prolyl isomerase activity, which catalyzes the isomerization of peptide bonds from trans form to cis form at proline residues and facilitates protein folding.

Cyclophilin A is a cytosolic and highly abundant protein. The protein belongs to a family of isozymes, including cyclophilins B and C, and natural killer cell cyclophilin-related protein.[2][3][4] Major isoforms have been found throughout the cell, including the ER, and some are even secreted.

Cyclophilin A (CypA)

File:1awv.jpg
Cyclophilin A + HIV peptid (green), Human.

Cyclophilin A also known as peptidylprolyl isomerase A, which is found in the cytosol, has a beta barrel structure with two alpha helices and a beta-sheet. Other cyclophilins have similar structures to cyclophilin A. The cyclosporin-cyclophilin A complex inhibits a calcium/calmodulin-dependent phosphatase, calcineurin, the inhibition of which is thought to suppress organ rejection by halting the production of the pro-inflammatory molecules TNF alpha and interleukin 2.

Cyclophilin A is also known to be recruited by the Gag polyprotein during HIV-1 virus infection, and its incorporation into new virus particles is essential for HIV-1 infectivity.[5]

Cyclophilin D

Cyclophilin D, which is located in the matrix of mitochondria, is only a modulatory, but not structural component of the mitochondrial permeability transition pore.[6][7] The pore opening raises the permeability of the mitochondrial inner membrane, allows influx of cytosolic molecules into the mitochondrial matrix, increases the matrix volume, and disrupts the mitochondrial outer membrane. As a result, the mitochondria fall into a functional disorder, so the opening of the pore plays an important role in cell death. Cyclophilin D is thought to regulate the opening of the pore because cyclosporin A, which binds to CyP-D, inhibits the pore opening.

However, mitochondria obtained from the cysts of Artemia franciscana, do not exhibit the mitochondrial permeability transition pore [8][9]

Clinical significance

Diseases

Drug targets

Cyclophilin inhibitors are being developed to treat neurodegenerative diseases.[10]

Examples

Human genes encoding proteins containing the cyclophilin type peptidyl-prolyl cis-trans isomerase domain include:

References

  1. ↑ Stamnes MA, Rutherford SL, Zuker CS (September 1992). "Cyclophilins: a new family of proteins involved in intracellular folding". Trends Cell Biol. 2 (9): 272–6. doi:10.1016/0962-8924(92)90200-7. PMID 14731520.
  2. ↑ Trandinh CC, Pao GM, Saier MH (December 1992). "Structural and evolutionary relationships among the immunophilins: two ubiquitous families of peptidyl-prolyl cis-trans isomerases". FASEB J. 6 (15): 3410–20. PMID 1464374.
  3. ↑ Galat A (September 1993). "Peptidylproline cis-trans-isomerases: immunophilins". Eur. J. Biochem. 216 (3): 689–707. doi:10.1111/j.1432-1033.1993.tb18189.x. PMID 8404888.
  4. ↑ Hacker J, Fischer G (November 1993). "Immunophilins: structure-function relationship and possible role in microbial pathogenicity". Mol. Microbiol. 10 (3): 445–56. doi:10.1111/j.1365-2958.1993.tb00917.x. PMID 7526121.
  5. ↑ Thali M, Bukovsky A, Kondo E, et al. (24 November 1994). "Functional association of cyclophilin A with HIV-1 virions". Nature. 372 (6504): 363–365. doi:10.1038/372363a0. PMID 7969495.
  6. ↑ Basso E, Fante L, Fowlkes J, Petronilli V, Forte MA, Bernardi P (May 2005). "Properties of the permeability transition pore in mitochondria devoid of Cyclophilin D". J. Biol. Chem. 280 (19): 18558–61. doi:10.1074/jbc.C500089200. PMID 15792954.
  7. ↑ Doczi J, Turiák L, Vajda S, et al. (February 2011). "Complex contribution of cyclophilin D to Ca2+-induced permeability transition in brain mitochondria, with relation to the bioenergetic state". J. Biol. Chem. 286 (8): 6345–53. doi:10.1074/jbc.M110.196600. PMC 3057831. PMID 21173147.
  8. ↑ Menze MA, Hutchinson K, Laborde SM, Hand SC (July 2005). "Mitochondrial permeability transition in the crustacean Artemia franciscana: absence of a calcium-regulated pore in the face of profound calcium storage". Am. J. Physiol. Regul. Integr. Comp. Physiol. 289 (1): R68–76. doi:10.1152/ajpregu.00844.2004. PMID 15718386.
  9. ↑ Konràd C, Kiss G, Töröcsik B, et al. (March 2011). "A distinct sequence in the adenine nucleotide translocase from Artemia franciscana embryos is associated with insensitivity to bongkrekate and atypical effects of adenine nucleotides on Ca2+ uptake and sequestration". FEBS J. 278 (5): 822–36. doi:10.1111/j.1742-4658.2010.08001.x. PMID 21205213.
  10. ↑ J&J targets degenerative diseases in cyclophilin inhibitor partnership. Dan Stanton. 08-Dec-2015

External links

Retrieved from "https://www.wikidoc.org/index.php?title=Cyclophilin&oldid=1421498"