Ureaplasma urealyticum laboratory findings: Difference between revisions
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==Overview== | ==Overview== | ||
Microbial culture method and/or [[PCR]]-based techniques are the two reliable methods for making a diagnosis of Ureaplasma colonization/infection. [[PCR]]-based techniques are more sensitive than culture | Microbial culture method and/or [[PCR]]-based techniques are the two reliable methods for making a diagnosis of Ureaplasma colonization/infection. [[PCR]]-based techniques are more sensitive than culture.<ref name="pmid 26518581">{{cite journal| author=Resch B, Gutmann C, Reiterer F, Luxner J, Urlesberger B| title=Neonatal Ureaplasma urealyticum colonization increases pulmonary and cerebral morbidity despite treatment with macrolide antibiotics. | journal=Infection | year= 2016 | volume= 44 | issue= 3 | pages= 323-7 | pmid= 26518581 | doi=10.1007/s15010-015-0858-7 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=26518581 }} </ref><ref name="pmid26668114">{{cite journal| author=Yoneda N, Yoneda S, Niimi H, Ueno T, Hayashi S, Ito M et al.| title=Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor. | journal=Am J Reprod Immunol | year= 2016 | volume= 75 | issue= 2 | pages= 112-25 | pmid=26668114 | doi=10.1111/aji.12456 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=26668114 }} </ref><ref name="pmid8496757">{{cite journal| author=Ollikainen J, Hiekkaniemi H, Korppi M, Sarkkinen H, Heinonen K| title=Ureaplasma urealyticum infection associated with acute respiratory insufficiency and death in premature infants. | journal=J Pediatr | year= 1993 | volume= 122 | issue= 5 Pt 1 | pages= 756-60 | pmid=8496757 | doi= | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=8496757 }} </ref> | ||
==Laboratory Findings== | ==Laboratory Findings== | ||
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]Associate Editor(s)-in-Chief: Fatimo Biobaku M.B.B.S [2]
Overview
Microbial culture method and/or PCR-based techniques are the two reliable methods for making a diagnosis of Ureaplasma colonization/infection. PCR-based techniques are more sensitive than culture.[1][2][3]
Laboratory Findings
Colonization and infection with Ureaplasma species are diagnosed based on culture results and/or PCR.[4][5][6][7][2][8][3]
Microscopy
Culture method: Ureaplasma species have been cultured from different sites of the body of infants such as the blood, CSF, nasopharynx, endotracheal secretions, gastric aspirates, pleural fluid, lung and brain tissue. Ureaplasma species are also the most common organism isolated from infected amniotic fluid and placenta. Culture from sites such as the urogenital tract, rectum, and joint aspirate, has been documented. Conventional bacteriologic culture methods cannot identify these species and special culture for Mycoplasma is often used.
Molecular-based test
PCR-based method: This is a rapid and more sensitive technique for detection of Ureaplasma species compared to microbial culture method.
References
- ↑ Resch B, Gutmann C, Reiterer F, Luxner J, Urlesberger B (2016). "Neonatal Ureaplasma urealyticum colonization increases pulmonary and cerebral morbidity despite treatment with macrolide antibiotics". Infection. 44 (3): 323–7. doi:10.1007/s15010-015-0858-7. PMID 26518581 26518581 Check
|pmid=value (help). - ↑ 2.0 2.1 Yoneda N, Yoneda S, Niimi H, Ueno T, Hayashi S, Ito M; et al. (2016). "Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor". Am J Reprod Immunol. 75 (2): 112–25. doi:10.1111/aji.12456. PMID 26668114.
- ↑ 3.0 3.1 Ollikainen J, Hiekkaniemi H, Korppi M, Sarkkinen H, Heinonen K (1993). "Ureaplasma urealyticum infection associated with acute respiratory insufficiency and death in premature infants". J Pediatr. 122 (5 Pt 1): 756–60. PMID 8496757.
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- ↑
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- ↑ Ollikainen J, Hiekkaniemi H, Korppi M, Katila ML, Heinonen K (1993). "Ureaplasma urealyticum cultured from brain tissue of preterm twins who died of intraventricular hemorrhage". Scand J Infect Dis. 25 (4): 529–31. PMID 8248756.