Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase 2.
Clinical significance
Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.[3]
Interactions
Ribosomal protein L5 has been shown to interact with:
↑Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM (January 2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics. 83 (1): 153–67. doi:10.1016/s0888-7543(03)00235-0. PMID14667819.
↑Boldyreff B, Issinger OG (February 1997). "A-Raf kinase is a new interacting partner of protein kinase CK2 beta subunit". FEBS Lett. 403 (2): 197–9. doi:10.1016/s0014-5793(97)00010-0. PMID9042965.
↑Kim JM, Cha JY, Marshak DR, Bae YS (September 1996). "Interaction of the beta subunit of casein kinase II with the ribosomal protein L5". Biochem. Biophys. Res. Commun. 226 (1): 180–6. doi:10.1006/bbrc.1996.1330. PMID8806611.
↑Ahn BH, Kim TH, Bae YS (October 2001). "Mapping of the interaction domain of the protein kinase CKII beta subunit with target proteins". Mol. Cells. 12 (2): 158–63. PMID11710515.
Hirano K, Ito M, Hartshorne DJ (1995). "Interaction of the ribosomal protein, L5, with protein phosphatase type 1". J. Biol. Chem. 270 (34): 19786–90. doi:10.1074/jbc.270.34.19786. PMID7649987.
Ogata K, Kurahashi A, Nishiyama C, Terao K (1994). "Presence of role of the 5SrRNA-L5 protein complex (5SRNP) in the threonyl- and histidyl-tRNA synthetase complex in rat liver cytosol". Biochim. Biophys. Acta. 1218 (3): 388–400. doi:10.1016/0167-4781(94)90192-9. PMID8049265.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID8125298.
Michael WM, Dreyfuss G (1996). "Distinct domains in ribosomal protein L5 mediate 5 S rRNA binding and nucleolar localization". J. Biol. Chem. 271 (19): 11571–4. doi:10.1074/jbc.271.19.11571. PMID8626719.
Kim JM, Cha JY, Marshak DR, Bae YS (1996). "Interaction of the beta subunit of casein kinase II with the ribosomal protein L5". Biochem. Biophys. Res. Commun. 226 (1): 180–6. doi:10.1006/bbrc.1996.1330. PMID8806611.
Boldyreff B, Issinger OG (1997). "A-Raf kinase is a new interacting partner of protein kinase CK2 beta subunit". FEBS Lett. 403 (2): 197–9. doi:10.1016/S0014-5793(97)00010-0. PMID9042965.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID9373149.
Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (1998). "A map of 75 human ribosomal protein genes". Genome Res. 8 (5): 509–23. doi:10.1101/gr.8.5.509. PMID9582194.
Guerra B, Issinger OG (1998). "p53 and the ribosomal protein L5 participate in high molecular mass complex formation with protein kinase CK2 in murine teratocarcinoma cell line F9 after serum stimulation and cisplatin treatment". FEBS Lett. 434 (1–2): 115–20. doi:10.1016/S0014-5793(98)00962-4. PMID9738462.
Park JW, Bae YS (1999). "Phosphorylation of ribosomal protein L5 by protein kinase CKII decreases its 5S rRNA binding activity". Biochem. Biophys. Res. Commun. 263 (2): 475–81. doi:10.1006/bbrc.1999.1345. PMID10491318.
Rosorius O, Fries B, Stauber RH, Hirschmann N, Bevec D, Hauber J (2000). "Human ribosomal protein L5 contains defined nuclear localization and export signals". J. Biol. Chem. 275 (16): 12061–8. doi:10.1074/jbc.275.16.12061. PMID10766838.