Sodium channel

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]

Overview

Sodium channels (also known as "voltage-gated sodium channels") are integral membrane proteins that conduct sodium ions (Na⁺) through a cell's plasma membrane. Many of the ionotropic receptors are also able to conduct sodium ions. In excitable cells such as neurons and myocytes, sodium channels are responsible for the rising phase of action potentials.

Structure

Diagram of a voltage-sensitive sodium channel α-subunit. G - glycosylation, P - phosphorylation, S - ion selectivity, I - inactivation, positive (+) charges in S4 are important for transmembrane voltage sensing.

Sodium channels can often be isolated from cells as a complex of two types of protein subunits, α and β. An α subunit forms the core of the channel. When the α subunit protein is expressed by a cell, it is able to form channels which conduct Na⁺ in a voltage-gated way, even if β subunits are not expressed. When β subunits assemble with α subunits the resulting complex can display altered voltage dependence and cellular localization.

The α-subunit has four repeat domains, labeled I through IV, each containing six membrane-spanning regions, labeled S1 through S6. The highly conserved S4 region acts as the channel's voltage sensor. The voltage sensitivity of this channel is due to positive amino acids located at every third position. When stimulated by a change in transmembrane voltage, this region moves toward the extracellular side of the cell membrane, allowing the channel to become permeable to ions. The ions are conducted through a pore, which can be broken into two regions. The more external (i.e., more extracellular) portion of the pore is formed by the "P-loops" (the region between S5 and S6) of the four domains. This region is the most narrow part of the pore and is responsible for its ion selectivity. The inner portion (i.e., more cytoplasmic) of the pore is formed by the combined S5 and S6 regions of the four domains. The region linking domains III and IV is also important for channel function. This region plugs the channel after prolonged activation, inactivating it.

Gating

Voltage-gated sodium channels have three types of states: deactivated (closed), activated (open), and inactivated (closed). Channels in the deactivated state are thought to be blocked on their intracellular side by an "activation gate", which is removed in response to stimulation that opens the channel. The ability to inactivate is thought to be due to a tethered plug (formed by domains III and IV of the alpha subunit), called an inactivation gate, that blocks the inside of the channel shortly after it has been activated. During an action potential the channel remains inactivated for a few milliseconds after depolarization. The inactivation is removed when the membrane potential of the cell repolarizes following the falling phase of the action potential. This allows the channels to be activated again during the next action potential. Genetic diseases that alter Na⁺ channel inactivation cause muscle stiffness because of the introduction of a window current.

The temporal behaviour of sodium channels can be described by a Markovian scheme or by the Hodgkin-Huxley-type formalism. In the former scheme, each channel occupies a distinct state with differential equations describing transitions between states; in the latter, the channels are treated as a population that are affected by three independent gating variables. Each of these variables can attain a value between 1 (fully permeant to ions) and 0 (fully non-permeant), the product of these variables yielding the percentage of conducting channels.

Impermeability to other ions

The pore of sodium channels contains a selectivity filter made of negatively charged amino acid residues, which attract the positive Na⁺ ion and keep out negatively charged ions such as chloride. The cations flow into a more constricted part of the pore that is 0.3 by 0.5 nm wide, which is just large enough to allow a single Na⁺ ion with a water molecule associated to pass through. The larger K⁺ ion cannot fit through this area. Differently sized ions also cannot interact as well with the negatively charged glutamic acid residues that line the pore.

Diversity

Sodium channels normally consist of an alpha subunit which forms the ion conduction pore and one to two beta subunits which have several functions including modulation of channel gating. Expression of the alpha subunit alone is sufficient to produce a functional channel.

Alpha subunits

Figure 1. Likely evolutionary relationship of the nine known human sodium channels.

The family of sodium channels has nine known members, with amino acid identity >50% in the transmembrane and extracellular loop regions. A standardized nomenclature for sodium channels is currently used and is maintain by the IUPHAR.

The proteins of these channels are named Na_v1.1 through Na_v1.9. The gene names are referred to as SCN1A through SCN11A (the SCN6/7A gene is part of the Na_x sub-family and has uncertain function). The likely evolutionary relationship between these channels, based on the similarity of their amino acid sequences, is shown in figure 1. The individual sodium channels are distinguished not only by differences in their sequence but also by their kinetics and expression profiles. Some of this data is summarized in table 1, below.

Table 1. Nomenclature and some function of voltage-gated sodium channels

Protein name	Gene	Auxiliary subunits	Expression profile	Associated human channelopathies
Navα1.1	SCN1A	β1,β2,β3,β4	Central neurons and cardiac myocytes	Inherited febrile epilepsy, GEFS and myoclonic epilepsy
Navα1.2	SCN2A	β1,β2,β3,β4	Central neurons	inherited febrile seizures and epilepsy
Navα1.3	SCN3A	β1,β3	Central neurons and cardiac myocytes	none known
Navα1.4	SCN4A	β1	Skeletal muscle	hyperkalemic periodic paralysis, Paramyotonia congenita, and potassium-aggravated myotonia
Navα1.5	SCN5A	β1,β2,β3,β4	Central neurons, cardiac myocytes	Long QT Syndrome, Brugada syndrome, and idiopathic ventricular fibrillation
Navα1.6	SCN8A	β1,β2	Central neurons, dorsal root ganglia, peripheral neurons	none known
Navα1.7	SCN9A	β1,β2	Dorsal root ganglia, sympathetic neurons, Schwann cells, and neuroendocrine cells	Erythromelalgia and Channelopathy-associated insensitivity to pain
Navα1.8	SCN10A	unknown	Dorsal root ganglia	none known
Navα1.9	SCN11A	unknown	Dorsal root ganglia	none known

Beta subunits

In addition to regulating channel gating, sodium channel beta subunits also modulate channel expression and form links to the intracelluar cytoskeleton and extracellular matrix.

Protein name	Gene link
Navβ1	SCN1B
Navβ2	SCN2B
Navβ3	SCN3B
Navβ4	SCN4B

Role in action potential

See main article: Action potential

Voltage-gated sodium channels play an important role in action potentials. If enough channels open when there is a change in the cell's membrane potential, a small but significant number of Na⁺ ions will move into the cell down their electrochemical gradient, further depolarizing the cell. Thus, the more Na⁺ channels localized in a region of a cell's membrane, the faster the action potential will propagate, and the more excitable that area of the cell will be. This is an example of a positive feedback loop. The ability of these channels to assume a closed-inactivated state causes the refractory period and is critical for the propagation of action potentials down an axon.

Na⁺ channels both open and close more quickly than K⁺ channels, producing an influx of positive charge (Na⁺) toward the beginning of the action potential and an efflux (K⁺) toward the end.

Ligands

Blockers

Extracellular

The following naturally produced substances block sodium channels by binding to and occluding the extracellular pore opening of the channel:

• Alkaloid based toxins
  • tetrodotoxin (TTX)
  • saxitoxin

Intracellular

Drugs which block sodium channels by blocking from the intracellular side of the channel:

• Local anesthetics
• Class I antiarrhythmic agents
• Some anticonvulsants

Activators

The following naturally produced substances persistently activate (open) sodium channels:

• Alkaloid based toxins
  • veratridine
  • batrachotoxin
  • aconitine
• Diterpene based toxins
  • grayanotoxin

Gating modifiers

The following toxins modify the gating of sodium channels:

• Peptide based toxins
  • μ-conotoxin
  • δ-atracotoxin

References

• Eric R. Kandel, Schwartz JH, Jessell TM. Principles of Neural Science, 4th ed., pp.154-169. McGraw-Hill, New York (2000). ISBN 0-8385-7701-6
• Bertil Hille Ion channels of excitable membranes, 3rd ed., Sinauer Associates, Sunderland, MA (2001). ISBN 0-87893-321-2

See also

• Ion channels
• Calcium channels
• Potassium channels
• Resting ion channels
• Epithelial sodium channel

External links

• Sodium+Channels at the US National Library of Medicine Medical Subject Headings (MeSH)