Discoidin domain-containing receptor 2, also known as CD167b (cluster of differentiation 167b), is a protein that in humans is encoded by the DDR2 gene.[1] Discoidin domain-containing receptor 2 is a receptor tyrosine kinase (RTK).
RTKs play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. In the case of DDR2, the ligand is collagen which binds to its extracellular discoidin domain.[2] This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. DDR2 has been associated with a number of diseases including fibrosis and cancer.[3]
Structure
RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain.[1]
Gene
Alternative splicing in the 5' UTR of the DDR2 gene results in multiple transcript variants encoding the same protein.[1]
Interactions
DDR2 (gene) has been shown to interact with SHC1[4] and phosphorylate Shp2.[5] DDR2 also interacts with Integrin α1β1 and α2β1 by promoting their adhesion to collagen.[6]
↑Ikeda K, Wang LH, Torres R, Zhao H, Olaso E, Eng FJ, Labrador P, Klein R, Lovett D, Yancopoulos GD, Friedman SL, Lin HC (May 2002). "Discoidin domain receptor 2 interacts with Src and Shc following its activation by type I collagen". J. Biol. Chem. 277 (21): 19206–12. doi:10.1074/jbc.M201078200. PMID11884411.
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Vogel W, Gish GD, Alves F, Pawson T (1997). "The discoidin domain receptor tyrosine kinases are activated by collagen". Mol. Cell. 1 (1): 13–23. doi:10.1016/S1097-2765(00)80003-9. PMID9659899.
Mohan RR, Mohan RR, Wilson SE (2001). "Discoidin domain receptor (DDR) 1 and 2: collagen-activated tyrosine kinase receptors in the cornea". Exp. Eye Res. 72 (1): 87–92. doi:10.1006/exer.2000.0932. PMID11133186.
Ikeda K, Wang LH, Torres R, Zhao H, Olaso E, Eng FJ, Labrador P, Klein R, Lovett D, Yancopoulos GD, Friedman SL, Lin HC (2002). "Discoidin domain receptor 2 interacts with Src and Shc following its activation by type I collagen". J. Biol. Chem. 277 (21): 19206–12. doi:10.1074/jbc.M201078200. PMID11884411.
Faraci E, Eck M, Gerstmayer B, Bosio A, Vogel WF (2003). "An extracellular matrix-specific microarray allowed the identification of target genes downstream of discoidin domain receptors". Matrix Biol. 22 (4): 373–81. doi:10.1016/S0945-053X(03)00053-2. PMID12935821.
Leitinger B, Steplewski A, Fertala A (2004). "The D2 period of collagen II contains a specific binding site for the human discoidin domain receptor, DDR2". J. Mol. Biol. 344 (4): 993–1003. doi:10.1016/j.jmb.2004.09.089. PMID15544808.
Yang K, Kim JH, Kim HJ, Park IS, Kim IY, Yang BS (2005). "Tyrosine 740 phosphorylation of discoidin domain receptor 2 by Src stimulates intramolecular autophosphorylation and Shc signaling complex formation". J. Biol. Chem. 280 (47): 39058–66. doi:10.1074/jbc.M506921200. PMID16186108.
Leitinger B, Kwan AP (2006). "The discoidin domain receptor DDR2 is a receptor for type X collagen". Matrix Biol. 25 (6): 355–64. doi:10.1016/j.matbio.2006.05.006. PMID16806867.
Zhang W, Ding T, Zhang J, Su J, Li F, Liu X, Ma W, Yao L (2006). "Expression of discoidin domain receptor 2 (DDR2) extracellular domain in pichia pastoris and functional analysis in synovial fibroblasts and NIT3T3 cells". Mol. Cell. Biochem. 290 (1–2): 43–53. doi:10.1007/s11010-006-9136-4. PMID16967187.