Gcr1p gene transcriptions

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Associate Editor(s)-in-Chief: Henry A. Hoff

"The transcription regulators (Gcr1 and Gcr2) are responsible for inducing the expression of the glycolytic genes in S. cerevisiae [14, 15], Gcr1p binds to CT boxes (5′-CTTCC-3′) upstream of the glycolytic genes and Gcr2p acts as a co-activator by forming a complex with Gcr1p [16]."[1]

Human genes

Consensus sequences

The upstream activating sequence (UAS) for Gcr1p is 5'-CTTCC-3' for the transcriptional activator involved in the regulation of glycolysis [77].[2]

GCR1 samplings

Copying the apparent consensus sequence for Gcr1p CTTCC and putting it in "⌘F" finds four located between ZSCAN22 and six between ZNF497 and A1BG as can be found by the computer programs.

For the Basic programs (starting with SuccessablesE.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are looking for, and found:

  1. negative strand, negative direction, looking for CTTCC, 4, CTTCC at 3611, CTTCC at 2558, CTTCC at 1656, CTTCC at 1556.
  2. positive strand, negative direction, looking for CTTCC, 1, CTTCC at 3777.
  3. negative strand, positive direction, looking for CTTCC, 6, CTTCC at 4242, CTTCC at 1433, CTTCC at 1333, CTTCC at 1070, CTTCC at 734, CTTCC at 677.
  4. positive strand, positive direction, looking for CTTCC, 3, CTTCC at 4249, CTTCC at 3852, CTTCC at 210.
  5. inverse complement, negative strand, negative direction, looking for GGAAG, 1, GGAAG at 619.
  6. inverse complement, positive strand, negative direction, looking for GGAAG, 7, GGAAG at 3909, GGAAG at 3610, GGAAG at 2730, GGAAG at 2557, GGAAG at 331, GGAAG at 241, GGAAG at 81.
  7. inverse complement, negative strand, positive direction, looking for GGAAG, 9, GGAAG at 4263, GGAAG at 4248, GGAAG at 3871, GGAAG at 3762, GGAAG at 3667, GGAAG at 2784, GGAAG at 2748, GGAAG at 1594, GGAAG at 209.
  8. inverse complement, positive strand, positive direction, looking for GGAAG, 20, GGAAG at 4241, GGAAG at 4061, GGAAG at 3312, GGAAG at 2582, GGAAG at 1516, GGAAG at 1432, GGAAG at 1405, GGAAG at 1332, GGAAG at 1305, GGAAG at 1264, GGAAG at 1153, GGAAG at 1069, GGAAG at 1012, GGAAG at 928, GGAAG at 828, GGAAG at 760, GGAAG at 733, GGAAG at 676, GGAAG at 592, GGAAG at 456.

GCR (4560-2846) UTRs

  1. Negative strand, negative direction: CTTCC at 3611.
  2. Positive strand, negative direction: GGAAG at 3909, GGAAG at 3610, CTTCC at 3777.

GCR negative direction (2811-2596) proximal promoters

  1. Positive strand, negative direction: GGAAG at 2730.

GCR positive direction (4265-4050) proximal promoters

  1. Negative strand, positive direction: GGAAG at 4263, GGAAG at 4248, CTTCC at 4242.
  2. Positive strand, positive direction: CTTCC at 4249, GGAAG at 4241, GGAAG at 4061.

GCR negative direction (2596-1) distal promoters

  1. Negative strand, negative direction: CTTCC at 2558, CTTCC at 1656, CTTCC at 1556, GGAAG at 619.
  2. Positive strand, negative direction: GGAAG at 2557, GGAAG at 331, GGAAG at 241, GGAAG at 81.

GCR positive direction (4050-1) distal promoters

  1. Negative strand, positive direction: GGAAG at 3871, GGAAG at 3762, GGAAG at 3667, GGAAG at 2784, GGAAG at 2748, GGAAG at 1594, CTTCC at 1433, CTTCC at 1333, CTTCC at 1070, CTTCC at 734, CTTCC at 677, GGAAG at 209.
  2. Positive strand, positive direction: CTTCC at 3852, GGAAG at 3312, GGAAG at 2582, GGAAG at 1516, GGAAG at 1432, GGAAG at 1405, GGAAG at 1332, GGAAG at 1305, GGAAG at 1264, GGAAG at 1153, GGAAG at 1069, GGAAG at 1012, GGAAG at 928, GGAAG at 828, GGAAG at 760, GGAAG at 733, GGAAG at 676, GGAAG at 592, GGAAG at 456, CTTCC at 210.

GCR random dataset samplings

  1. GCRr0: 4, CTTCC at 3519, CTTCC at 1594, CTTCC at 1119, CTTCC at 122.
  2. GCRr1: 6, CTTCC at 4075, CTTCC at 4067, CTTCC at 2093, CTTCC at 732, CTTCC at 650, CTTCC at 293.
  3. GCRr2: 6, CTTCC at 3970, CTTCC at 3395, CTTCC at 2291, CTTCC at 923, CTTCC at 646, CTTCC at 161.
  4. GCRr3: 7, CTTCC at 3954, CTTCC at 3565, CTTCC at 2929, CTTCC at 1861, CTTCC at 1844, CTTCC at 1822, CTTCC at 1035.
  5. GCRr4: 5, CTTCC at 4194, CTTCC at 3692, CTTCC at 1461, CTTCC at 824, CTTCC at 590.
  6. GCRr5: 3, CTTCC at 2017, CTTCC at 1668, CTTCC at 1472.
  7. GCRr6: 4, CTTCC at 3592, CTTCC at 2755, CTTCC at 2305, CTTCC at 697.
  8. GCRr7: 6, CTTCC at 4126, CTTCC at 3084, CTTCC at 3003, CTTCC at 1900, CTTCC at 1200, CTTCC at 1021.
  9. GCRr8: 8, CTTCC at 4333, CTTCC at 4252, CTTCC at 3950, CTTCC at 2321, CTTCC at 1824, CTTCC at 1467, CTTCC at 1356, CTTCC at 633.
  10. GCRr9: 7, CTTCC at 3338, CTTCC at 3134, CTTCC at 2824, CTTCC at 1994, CTTCC at 699, CTTCC at 612, CTTCC at 126.
  11. GCRr0ci: 3, GGAAG at 2941, GGAAG at 2525, GGAAG at 1149.
  12. GCRr1ci: 8, GGAAG at 4363, GGAAG at 3669, GGAAG at 3562, GGAAG at 2466, GGAAG at 928, GGAAG at 690, GGAAG at 467, GGAAG at 462.
  13. GCRr2ci: 6, GGAAG at 4132, GGAAG at 4128, GGAAG at 4012, GGAAG at 3373, GGAAG at 2204, GGAAG at 45.
  14. GCRr3ci: 5, GGAAG at 4405, GGAAG at 4312, GGAAG at 3149, GGAAG at 2546, GGAAG at 1319.
  15. GCRr4ci: 5, GGAAG at 3899, GGAAG at 3313, GGAAG at 2554, GGAAG at 1865, GGAAG at 575.
  16. GCRr5ci: 7, GGAAG at 3167, GGAAG at 3004, GGAAG at 2390, GGAAG at 2344, GGAAG at 2237, GGAAG at 1726, GGAAG at 1192.
  17. GCRr6ci: 5, GGAAG at 3898, GGAAG at 3833, GGAAG at 2579, GGAAG at 1503, GGAAG at 1192.
  18. GCRr7ci: 4, GGAAG at 3710, GGAAG at 2971, GGAAG at 2433, GGAAG at 649.
  19. GCRr8ci: 6, GGAAG at 4382, GGAAG at 4168, GGAAG at 3643, GGAAG at 3214, GGAAG at 2921, GGAAG at 2756.
  20. GCRr9ci: 2, GGAAG at 3485, GGAAG at 2177.

GCRr arbitrary (evens) (4560-2846) UTRs

  1. GCRr0: CTTCC at 3519.
  2. GCRr2: CTTCC at 3970, CTTCC at 3395.
  3. GCRr4: CTTCC at 4194, CTTCC at 3692.
  4. GCRr6: CTTCC at 3592.
  5. GCRr8: CTTCC at 4333, CTTCC at 4252, CTTCC at 3950.
  6. GCRr0ci: GGAAG at 2941.
  7. GCRr2ci: GGAAG at 4132, GGAAG at 4128, GGAAG at 4012, GGAAG at 3373.
  8. GCRr4ci: GGAAG at 3899, GGAAG at 3313.
  9. GCRr6ci: GGAAG at 3898, GGAAG at 3833.
  10. GCRr8ci: GGAAG at 4382, GGAAG at 4168, GGAAG at 3643, GGAAG at 3214, GGAAG at 2921.

GCRr alternate (odds) (4560-2846) UTRs

  1. GCRr1: CTTCC at 4075, CTTCC at 4067.
  2. GCRr3: CTTCC at 3954, CTTCC at 3565, CTTCC at 2929.
  3. GCRr7: CTTCC at 4126, CTTCC at 3084, CTTCC at 3003.
  4. GCRr9: CTTCC at 3338, CTTCC at 3134.
  5. GCRr1ci: GGAAG at 4363, GGAAG at 3669, GGAAG at 3562.
  6. GCRr3ci: GGAAG at 4405, GGAAG at 4312, GGAAG at 3149.
  7. GCRr5ci: GGAAG at 3167, GGAAG at 3004.
  8. GCRr7ci: GGAAG at 3710, GGAAG at 2971.
  9. GCRr9ci: GGAAG at 3485.

GCRr arbitrary positive direction (odds) (4445-4265) core promoters

  1. GCRr1ci: GGAAG at 4363.
  2. GCRr3ci: GGAAG at 4405, GGAAG at 4312.

GCRr alternate positive direction (evens) (4445-4265) core promoters

  1. GCRr8: CTTCC at 4333.
  2. GCRr8ci: GGAAG at 4382.

GCRr arbitrary negative direction (evens) (2811-2596) proximal promoters

  1. GCRr6: CTTCC at 2755.
  2. GCRr8ci: GGAAG at 2756.

GCRr arbitrary positive direction (odds) (4265-4050) proximal promoters

  1. GCRr1: CTTCC at 4075, CTTCC at 4067.
  2. GCRr7: CTTCC at 4126.

GCRr alternate positive direction (evens) (4265-4050) proximal promoters

  1. GCRr4: CTTCC at 4194.
  2. GCRr8: CTTCC at 4252.
  3. GCRr2ci: GGAAG at 4132, GGAAG at 4128.
  4. GCRr8ci: GGAAG at 4168.

GCRr arbitrary negative direction (evens) (2596-1) distal promoters

  1. GCRr0: CTTCC at 1594, CTTCC at 1119, CTTCC at 122.
  2. GCRr2: CTTCC at 2291, CTTCC at 923, CTTCC at 646, CTTCC at 161.
  3. GCRr4: CTTCC at 1461, CTTCC at 824, CTTCC at 590.
  4. GCRr6: CTTCC at 2305, CTTCC at 697.
  5. GCRr8: CTTCC at 2321, CTTCC at 1824, CTTCC at 1467, CTTCC at 1356, CTTCC at 633.
  6. GCRr0ci: GGAAG at 2525, GGAAG at 1149.
  7. GCRr2ci: GGAAG at 2204, GGAAG at 45.
  8. GCRr4ci: GGAAG at 2554, GGAAG at 1865, GGAAG at 575.
  9. GCRr6ci: GGAAG at 2579, GGAAG at 1503, GGAAG at 1192.

GCRr alternate negative direction (odds) (2596-1) distal promoters

  1. GCRr1: CTTCC at 2093, CTTCC at 732, CTTCC at 650, CTTCC at 293.
  2. GCRr3: CTTCC at 1861, CTTCC at 1844, CTTCC at 1822, CTTCC at 1035.
  3. GCRr5: CTTCC at 2017, CTTCC at 1668, CTTCC at 1472.
  4. GCRr7: CTTCC at 1900, CTTCC at 1200, CTTCC at 1021.
  5. GCRr9: CTTCC at 1994, CTTCC at 699, CTTCC at 612, CTTCC at 126.
  6. GCRr1ci: GGAAG at 2466, GGAAG at 928, GGAAG at 690, GGAAG at 467, GGAAG at 462.
  7. GCRr3ci: GGAAG at 2546, GGAAG at 1319.
  8. GCRr5ci: GGAAG at 2390, GGAAG at 2344, GGAAG at 2237, GGAAG at 1726, GGAAG at 1192.
  9. GCRr7ci: GGAAG at 2433, GGAAG at 649.
  10. GCRr9ci: GGAAG at 2177.

GCRr arbitrary positive direction (odds) (4050-1) distal promoters

  1. GCRr1: CTTCC at 2093, CTTCC at 732, CTTCC at 650, CTTCC at 293.
  2. GCRr3: CTTCC at 3954, CTTCC at 3565, CTTCC at 2929, CTTCC at 1861, CTTCC at 1844, CTTCC at 1822, CTTCC at 1035.
  3. GCRr5: CTTCC at 2017, CTTCC at 1668, CTTCC at 1472.
  4. GCRr7: CTTCC at 3084, CTTCC at 3003, CTTCC at 1900, CTTCC at 1200, CTTCC at 1021.
  5. GCRr9: CTTCC at 3338, CTTCC at 3134, CTTCC at 2824, CTTCC at 1994, CTTCC at 699, CTTCC at 612, CTTCC at 126.
  6. GCRr1ci: GGAAG at 3669, GGAAG at 3562, GGAAG at 2466, GGAAG at 928, GGAAG at 690, GGAAG at 467, GGAAG at 462.
  7. GCRr3ci: GGAAG at 3149, GGAAG at 2546, GGAAG at 1319.
  8. GCRr5ci: GGAAG at 3167, GGAAG at 3004, GGAAG at 2390, GGAAG at 2344, GGAAG at 2237, GGAAG at 1726, GGAAG at 1192.
  9. GCRr7ci: GGAAG at 3710, GGAAG at 2971, GGAAG at 2433, GGAAG at 649.
  10. GCRr9ci: GGAAG at 3485, GGAAG at 2177.

GCRr alternate positive direction (evens) (4050-1) distal promoters

  1. GCRr0: CTTCC at 3519, CTTCC at 1594, CTTCC at 1119, CTTCC at 122.
  2. GCRr2: CTTCC at 3970, CTTCC at 3395, CTTCC at 2291, CTTCC at 923, CTTCC at 646, CTTCC at 161.
  3. GCRr4: CTTCC at 3692, CTTCC at 1461, CTTCC at 824, CTTCC at 590.
  4. GCRr6: CTTCC at 3592, CTTCC at 2755, CTTCC at 2305, CTTCC at 697.
  5. GCRr8: CTTCC at 3950, CTTCC at 2321, CTTCC at 1824, CTTCC at 1467, CTTCC at 1356, CTTCC at 633.
  6. GCRr0ci: GGAAG at 2941, GGAAG at 2525, GGAAG at 1149.
  7. GCRr2ci: GGAAG at 4012, GGAAG at 3373, GGAAG at 2204, GGAAG at 45.
  8. GCRr4ci: GGAAG at 3899, GGAAG at 3313, GGAAG at 2554, GGAAG at 1865, GGAAG at 575.
  9. GCRr6ci: GGAAG at 3898, GGAAG at 3833, GGAAG at 2579, GGAAG at 1503, GGAAG at 1192.
  10. GCRr8ci: GGAAG at 3643, GGAAG at 3214, GGAAG at 2921, GGAAG at 2756.

GCR analysis and results

Main article: Complex locus A1BG and ZNF497 § GCR1s

Gcr1p binds to CT boxes (5′-CTTCC-3′) upstream of the glycolytic genes and Gcr2p acts as a co-activator by forming a complex with Gcr1p [16]."[1]

Reals or randoms Promoters direction Numbers Strands Occurrences Averages (± 0.1)
Reals UTR negative 4 2 2.0 2.0 ± 1 (--1,+-3)
Randoms UTR arbitrary negative 23 10 2.3 2.2
Randoms UTR alternate negative 21 10 2.1 2.2
Reals Core negative 0 2 0 0
Randoms Core arbitrary negative 0 10 0 0
Randoms Core alternate negative 0 10 0 0
Reals Core positive 0 2 0 0
Randoms Core arbitrary positive 3 10 0.3 0.25
Randoms Core alternate positive 2 10 0.2 0.25
Reals Proximal negative 1 2 0.5 0.5 ± 0.5 (--0,+-1)
Randoms Proximal arbitrary negative 2 10 0.2 0.1
Randoms Proximal alternate negative 0 10 0 0.1
Reals Proximal positive 6 2 3 3 ± 0 (-+3,++3)
Randoms Proximal arbitrary positive 2 10 0.2 0.35
Randoms Proximal alternate positive 5 10 0.5 0.35
Reals Distal negative 8 2 4 4 ± 0 (--4,+-4)
Randoms Distal arbitrary negative 27 10 2.7 3.0
Randoms Distal alternate negative 33 10 3.3 3.0
Reals Distal positive 32 2 16 16 ± 4 (-+12,++20)
Randoms Distal arbitrary positive 49 10 4.9 4.7
Randoms Distal alternate positive 45 10 4.5 4.7

Comparison:

The occurrences of real GCR UTRs are outside the randoms, the proximals and distals are greater than the randoms. This suggests that the real responsive element consensus sequences are likely active or activable.

Pathways

There are two principal signal transduction pathways involving the G protein-coupled receptors:

See also

References

  1. ↑ 1.0 1.1 Doblin Sandai, Yasser Tabana and Rosline Sandai (5 November 2018). Doblin Sandai, ed. Carbon Sources Attribute to Pathogenicity in Candida albicans, in Candida albicans. IntechOpen. doi:10.5772/intechopen.80211. Retrieved 7 January 2021.
  2. ↑ Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.
  3. ↑ Gilman AG (1987). "G proteins: transducers of receptor-generated signals". Annual Review of Biochemistry. 56 (1): 615–49. doi:10.1146/annurev.bi.56.070187.003151. PMID 3113327.

External links

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