U box gene transcriptions

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Associate Editor(s)-in-Chief: Henry A. Hoff

"The U box is a domain of ∼70 amino acids that is present in proteins from yeast to humans."[1]

"The prototype U box protein, yeast Ufd2, was identified as a ubiquitin chain assembly factor that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin-protein ligase (E3) to catalyze ubiquitin chain formation on artificial substrates."[1]

"The UFD2 protein and its homologs in other eukaryotes share a conserved domain designated the ‘U box’."[2]

"The U box mediates the interaction of UFD2 with ubiquitin conjugated proteins [...] the U box is a derived version of the RING-finger domain that lacks the hallmark metal-chelating residues of the latter [5,6] but is likely to function similarly to the RING-finger in mediating ubiquitin-conjugation of protein substrates."[2]

Tomato U boxes

"The promoter region of the identified 62 tomato U-box E3 genes was analyzed using PlantCARE database50 for the presence of the cis-regulatory elements [...]. The cis-regulatory elements, the binding sites for transcription factors, carry information to regulate the gene expression in certain environmental responses or biological pathways. The occurrence frequency of the elements was represented as a word cloud image [...]. We found jasmonic acid-responsive elements (TGACG, CGTCA), MYB binding site involved in drought induction (TAACTG), gibberellin responsive elements (CCTTTTG,AAACAGA), endosperm expression (TGTGTCA), abscisic acid-responsive elements (CACGTG), ethylene responsive elements (ATTTCAAA), heat-responsive elements (AAAAAATTTC), defense and stress-responsive elements (ATTTTCTTCA), circadian control elements (CAANNNNATC) abundantly in U-box E3 family. The presence of these elements predicts the notable participation of U-box E3 gene family members in hormonal pathways and stress responses. We have noticed jasmonic acid, drought inductive, endosperm expression, defense, stress-responsive and abscisic acid-responsive elements with the highest frequency compared to other elements in the U-box E3 gene family. It suggests the critical requirement of the U-box E3 ubiquitin ligase in various cellular pathways. However, we could not observe any definite pattern of cis-element appearance with the four classes of the U-box E3 gene family. A few genes were also noticed with the shoot (GATAatGATG) and root (TGACGTCA) specific elements, cell cycle regulation (CCCAACGGT), seed-specific element (CATGCATG) and alpha-amylase conserved elements (TATCCATCCATCC)."[3]

See also

References

  1. 1.0 1.1 Shigetsugu Hatakeyama, Masayoshi Yada, Masaki Matsumoto, Noriko Ishida and Kei-Ichi Nakayama (2001). "U Box Proteins as a New Family of Ubiquitin-Protein Ligases". The Journal of Biological Chemistry. 276: 33111–20. doi:10.1074/jbc.M102755200. Retrieved 2014-06-16. Unknown parameter |month= ignored (help)
  2. 2.0 2.1 L. Aravind and Eugene V. Koonin (2000). "The U box is a modified RING finger — a common domain in ubiquitination". Current Biology. 10 (4): R132–4. |access-date= requires |url= (help)
  3. Bhaskar Sharma & Joemar Taganna (12 June 2020). "Genome-wide analysis of the U-box E3 ubiquitin ligase enzyme gene family in tomato". Scientific Reports. 10 (9581). doi:10.1038/s41598-020-66553-1. Retrieved 27 August 2020.

External links

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