Rox1p gene transcriptions
Associate Editor(s)-in-Chief: Henry A. Hoff
"All promoters recognized by pol-II may require one or more UASs for regulated gene expression [36,37]."[1]
Human genes
Gene expressions
"Inducible promoters can initiate a dramatic change in the transcriptional level of their corresponding genes upon the presence or absence of specific stimuli. In S. cerevisiae, these stimuli, or inducers, range from carbon sources, including glucose, galactose, sucrose, maltose, glycerol, acetate and ethanol, to environmental factors such as the temperature, pH, stress and light, to others, such as metabolites, amino acids, metal ions and hormones [9,10,11]."[1]
An inducer for the Rox1 transcription factor is Heme which functions for a Heme-dependent repressor of hypoxic genes.[1]
Interactions
Consensus sequences
"The upstream activating sequence (UAS) is located upstream of the core promoter and serves as a binding site for specific transcription activators."[1]
UAS for the Rox1p transcription factor is RRRTAACAAGAG.[1]
Binding site for
Complement copies
Inverse copies
Complement-inverse copies
Enhancer activity
Promoter occurrences
The UAS for Rox1p occurs in the S. cerevisiae HEM13, COX5B, ANB1, and CYC7 promoters.[1]
Hypotheses
- A1BG has no regulatory elements in either promoter.
- A1BG is not transcribed by a regulatory element.
- No regulatory element participates in the transcription of A1BG.
ROX samplings
Copying a responsive elements consensus sequence GGGTAA and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence (A/G)(A/G)(A/G)TAACAAGAG (starting with SuccessablesROX.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for (A/G)(A/G)(A/G)TAACAAGAG, 0.
- positive strand, negative direction, looking for (A/G)(A/G)(A/G)TAACAAGAG, 0.
- positive strand, positive direction, looking for (A/G)(A/G)(A/G)TAACAAGAG, 0.
- negative strand, positive direction, looking for (A/G)(A/G)(A/G)TAACAAGAG, 0.
- complement, negative strand, negative direction, looking for (C/T)(C/T)(C/T)ATTGTTCTC, 0.
- complement, positive strand, negative direction, looking for (C/T)(C/T)(C/T)ATTGTTCTC, 0.
- complement, positive strand, positive direction, looking for (C/T)(C/T)(C/T)ATTGTTCTC, 0.
- complement, negative strand, positive direction, looking for (C/T)(C/T)(C/T)ATTGTTCTC, 0.
- inverse complement, negative strand, negative direction, looking for CTCTTGTTA(C/T)(C/T)(C/T), 0.
- inverse complement, positive strand, negative direction, looking for CTCTTGTTA(C/T)(C/T)(C/T), 0.
- inverse complement, positive strand, positive direction, looking for CTCTTGTTA(C/T)(C/T)(C/T), 0.
- inverse complement, negative strand, positive direction, looking for CTCTTGTTA(C/T)(C/T)(C/T), 0.
- inverse negative strand, negative direction, looking for GAGAACAAT(A/G)(A/G)(A/G), 0.
- inverse positive strand, negative direction, looking for GAGAACAAT(A/G)(A/G)(A/G), 0.
- inverse positive strand, positive direction, looking for GAGAACAAT(A/G)(A/G)(A/G), 0.
- inverse negative strand, positive direction, looking for GAGAACAAT(A/G)(A/G)(A/G), 0.
Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.
See also
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check
|pmid=value (help). Retrieved 18 September 2020.