Gal4p gene transcriptions: Difference between revisions

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==Human genes==
==Human genes==
{{main|Human genes}}


==Interactions==
==Interactions==
{{main|Interaction gene transcriptions}}
"During the induction of galactose, the production of Gal4p can improve the PGAL1 activity 1000-fold [37]."<ref name=Tang/>


"During the induction of galactose, the production of Gal4p can improve the PGAL1 activity 1000-fold [37]."<ref name=Tang/>
"We used the yeast Gal4p two-hybrid system (Fields and Sternglanz, 1994) to identify proteins that physically interact with GLD-1. We recovered two identical cDNAs in two-hybrid screens [...]. One (OG2.3) using GLD-1 residues 84-341 and the other (CD13.1) using residues 273-457, both fused to the Gal4p DNA binding domain [...]."<ref name=Clifford>{{ cite journal
|author=Robert Clifford, Min-Ho Lee, Sudhir Nayak, Mitsue Ohmachi, Flav Giorgini and Tim Schedl
|title=FOG-2, a novel F-box containing protein, associates with the GLD-1 RNA binding protein and directs male sex determination in the ''C. elegans'' hermaphrodite germline
|journal=Development
|date=December 2000
|volume=127
|issue=24
|pages=5265-76
|url=https://dev.biologists.org/content/develop/127/24/5265.full.pdf
|arxiv=
|bibcode=
|doi=
|pmid=11076749
|accessdate=10 August 2020 }}</ref>


==Consensus sequences==
==Consensus sequences==
 
{{main|Consensus sequence gene transcriptions}}
Upstream activating sequence for Gal4p is 5'-CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG-3' [38].<ref name=Tang>{{ cite journal
Upstream activating sequence for Gal4p is 5'-CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG-3' [38].<ref name=Tang>{{ cite journal
|author=Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling
|author=Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling
Line 27: Line 43:
|pmid=32781665
|pmid=32781665
|accessdate=18 September 2020 }}</ref>
|accessdate=18 September 2020 }}</ref>
==Hypotheses==
{{main|Hypotheses}}
# A1BG has no Gal4ps in either promoter.
# A1BG is not transcribed by a Gal4p.
# Gal4p does not participate in the transcription of A1BG.


==Samplings==
==Samplings==


Copying 5'-CGGACCGC-3' in "⌘F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.
Copying 5'-CGGACCGC-3' in "⌘F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG (starting with SuccessablesGal4.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
# negative strand, negative direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
# negative strand, positive direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
# positive strand, negative direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
# positive strand, positive direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
# complement, negative strand, negative direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
# complement, negative strand, positive direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
# complement, positive strand, negative direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
# complement, positive strand, positive direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
# inverse complement, negative strand, negative direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
# inverse complement, negative strand, positive direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
# inverse complement, positive strand, negative direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
# inverse complement, positive strand, positive direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
# inverse negative strand, negative direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
# inverse negative strand, positive direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
# inverse positive strand, negative direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
# inverse positive strand, positive direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
==Acknowledgements==
The content on this page was first contributed by: Henry A. Hoff.


==See also==
==See also==
{{div col|colwidth=20em}}
{{div col|colwidth=20em}}
* [[A1BG gene transcription core promoters]]
* [[A1BG gene transcriptions]]
* [[A1BG regulatory elements and regions]]
* [[A1BG response element negative results]]
* [[A1BG response element positive results]]
* [[Complex locus A1BG and ZNF497]]
* [[Complex locus A1BG and ZNF497]]
* [[Transcription factor]]
{{Div col end}}
{{Div col end}}


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==External links==
==External links==
* [http://www.genome.jp/ GenomeNet KEGG database]
* [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene Home - Gene - NCBI]
* [http://www.ncbi.nlm.nih.gov/sites/gquery NCBI All Databases Search]
* [http://www.ncbi.nlm.nih.gov/ncbisearch/ NCBI Site Search]
* [http://www.ncbi.nlm.nih.gov/pccompound PubChem Public Chemical Database]


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[[Category:Resources last modified in September 2020]]
[[Category:Resources last modified in December 2020]]

Latest revision as of 22:28, 31 December 2020

Associate Editor(s)-in-Chief: Henry A. Hoff

"PGAL1 is often used repeatedly for the expression of different enzymes in the construction of metabolic pathways [13], so the gene copy number has to be increased to compensate for the insufficient promoter strength [8], and the utilization of too many galactose-inducible promoters may interfere with the metabolism of galactose due to the depletion of the transcription activator Gal4p [14]."[1]

Galactose-inducible "promoters have various conserved UASGAL sites, a 17-bp consensus sequence 5′-CCGNNNNNNNNNNNCGG-3′ that is recognized by the transcription activator Gal4p to control their expression activities [38]."[1]

Human genes

Main article: Human genes

Interactions

Main article: Interaction gene transcriptions

"During the induction of galactose, the production of Gal4p can improve the PGAL1 activity 1000-fold [37]."[1]

"We used the yeast Gal4p two-hybrid system (Fields and Sternglanz, 1994) to identify proteins that physically interact with GLD-1. We recovered two identical cDNAs in two-hybrid screens [...]. One (OG2.3) using GLD-1 residues 84-341 and the other (CD13.1) using residues 273-457, both fused to the Gal4p DNA binding domain [...]."[2]

Consensus sequences

Main article: Consensus sequence gene transcriptions

Upstream activating sequence for Gal4p is 5'-CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG-3' [38].[1]

Hypotheses

Main article: Hypotheses
  1. A1BG has no Gal4ps in either promoter.
  2. A1BG is not transcribed by a Gal4p.
  3. Gal4p does not participate in the transcription of A1BG.

Samplings

Copying 5'-CGGACCGC-3' in "⌘F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG (starting with SuccessablesGal4.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand, negative direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
  2. negative strand, positive direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
  3. positive strand, negative direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
  4. positive strand, positive direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
  5. complement, negative strand, negative direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
  6. complement, negative strand, positive direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
  7. complement, positive strand, negative direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
  8. complement, positive strand, positive direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
  9. inverse complement, negative strand, negative direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
  10. inverse complement, negative strand, positive direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
  11. inverse complement, positive strand, negative direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
  12. inverse complement, positive strand, positive direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
  13. inverse negative strand, negative direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
  14. inverse negative strand, positive direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
  15. inverse positive strand, negative direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
  16. inverse positive strand, positive direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

See also

References

  1. ↑ 1.0 1.1 1.2 1.3 Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.
  2. ↑ Robert Clifford, Min-Ho Lee, Sudhir Nayak, Mitsue Ohmachi, Flav Giorgini and Tim Schedl (December 2000). "FOG-2, a novel F-box containing protein, associates with the GLD-1 RNA binding protein and directs male sex determination in the C. elegans hermaphrodite germline" (PDF). Development. 127 (24): 5265–76. PMID 11076749. Retrieved 10 August 2020.

External links

Retrieved from "https://www.wikidoc.org/index.php?title=Gal4p_gene_transcriptions&oldid=1680971"