Cytokinin response regulator gene transcriptions: Difference between revisions
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For the Basic programs testing consensus sequence (A/G)AGATA (starting with SuccessablesARR1.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found: | For the Basic programs testing consensus sequence (A/G)AGATA (starting with SuccessablesARR1.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found: | ||
# negative strand, negative direction, looking for (A/G)AGATA, 1, AAGATA at 1525. | # negative strand, negative direction, looking for (A/G)AGATA, 1, AAGATA at 1525. | ||
# positive strand, negative direction, looking for | # positive strand, negative direction, looking for (A/G)AGATA, 3, AAGATA at 2177, AAGATA at 1595, AAGATA at 57. | ||
# positive strand, positive direction, looking for | # positive strand, positive direction, looking for (A/G)AGATA, 0. | ||
# negative strand, positive direction, looking for | # negative strand, positive direction, looking for (A/G)AGATA, 0. | ||
# inverse complement, negative strand, negative direction, looking for TATCT(C/T), | # inverse complement, negative strand, negative direction, looking for TATCT(C/T), 4, TATCTT at 4080, TATCTC at 1711, TATCTT at 356, TATCTT at 101. | ||
# inverse complement, positive strand, negative direction, looking for | # inverse complement, positive strand, negative direction, looking for TATCT(C/T), 1, TATCTT at 1732. | ||
# inverse complement, positive strand, positive direction, looking for | # inverse complement, positive strand, positive direction, looking for TATCT(C/T), 1, TATCTT at 2628. | ||
# inverse complement, negative strand, positive direction, looking for | # inverse complement, negative strand, positive direction, looking for TATCT(C/T), 0. | ||
===ARR12 UTRs=== | ===ARR12 UTRs=== | ||
{{main|UTR promoter gene transcriptions}} | {{main|UTR promoter gene transcriptions}} | ||
Negative strand, negative direction: TATCTT at 4080. | |||
===ARR12 | ===ARR12 distal promoters=== | ||
{{main| | {{main|Distal promoter gene transcriptions}} | ||
Negative strand, negative direction: TATCTC at 1711, AAGATA at 1525, TATCTT at 356, TATCTT at 101. | |||
Positive strand, negative direction: AAGATA at 2177, TATCTT at 1732, AAGATA at 1595, AAGATA at 57. | |||
Positive strand, positive direction: TATCTT at 2628. | |||
==Acknowledgements== | ==Acknowledgements== |
Revision as of 00:51, 28 April 2021
Associate Editor(s)-in-Chief: Henry A. Hoff
"Cytokinin fulfills its diverse roles in planta through a series of transcriptional responses."[1]
Human genes
Gene expressions
Interactions
"Cytokinin employs a two-component multi-step phosphorelay for its perception and signaling transduction12–14. In Arabidopsis, there are three cytokinin receptors (ARABIDOPSIS HISTIDINE KINASEs; AHK2, 3, 4) and eleven type-B response regulators (ARABIDOSPIS RESPONSE REGULATORs; B-ARRs)8,15."[1]
"The cytokinin transcriptional response centrally affects the family of ARRs. Type-B ARRs (B-ARRs) are transcription factors (TFs) with a GARP-like DNA binding domain at their C-termini and a receiver domain at their N-termini. Type-A ARRs (A-ARRs) are similar to the N-termini receiver domain of B-ARRs but do not possess a DNA binding domain."[1]
Consensus sequences
ARR1: AGATT(C/T), ARR10: (A/G)GATA(A/C)G, ARR12: (A/G)AGATA.[1]
Binding site for
Enhancer activity
Promoter occurrences
Hypotheses
- A1BG has no regulatory elements in either promoter.
- A1BG is not transcribed by a regulatory element.
- No regulatory element participates in the transcription of A1BG.
ARR1 Cytokinin samplings
Copying a responsive elements consensus sequence AGATT(C/T) and putting the sequence in "⌘F" finds one between ZNF497 and A1BG or one between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence AGATT(C/T) (starting with SuccessablesARR1.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for AGATT(C/T), 0.
- positive strand, negative direction, looking for AGATT(C/T), 1, AGATTC at 2452.
- positive strand, positive direction, looking for AGATT(C/T), 1, AGATTC at 122.
- negative strand, positive direction, looking for AGATT(C/T), 1, AGATTT at 2869.
- inverse complement, negative strand, negative direction, looking for (A/G)AATCT, 1, AAATCT at 50.
- inverse complement, positive strand, negative direction, looking for (A/G)AATCT, 0.
- inverse complement, positive strand, positive direction, looking for (A/G)AATCT, 0.
- inverse complement, negative strand, positive direction, looking for (A/G)AATCT, 2, GAATCT at 2725, GAATCT at 2243.
ARR1 distal promoters
Negative strand, negative direction: AAATCT at 50.
Positive strand, negative direction: AGATTC at 2452.
Negative strand, positive direction: AGATTT at 2869, GAATCT at 2725, GAATCT at 2243.
Positive strand, positive direction: AGATTC at 122.
ARR10 Cytokinin samplings
Copying a responsive elements consensus sequence (A/G)GATA(A/C)G and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence (A/G)GATA(A/C)G (starting with SuccessablesARR1.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for (A/G)GATA(A/C)G, 0.
- positive strand, negative direction, looking for AAAAAAAA, 0.
- positive strand, positive direction, looking for AAAAAAAA, 0.
- negative strand, positive direction, looking for AAAAAAAA, 0.
- inverse complement, negative strand, negative direction, looking for C(G/T)TATC(C/T), 0.
- inverse complement, positive strand, negative direction, looking for TTTTTTTT, 0.
- inverse complement, positive strand, positive direction, looking for TTTTTTTT, 0.
- inverse complement, negative strand, positive direction, looking for TTTTTTTT, 0.
ARR10 UTRs
ARR10 core promoters
ARR10 proximal promoters
ARR10 distal promoters
ARR12 Cytokinin samplings
Copying a responsive elements consensus sequence (A/G)AGATA and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence (A/G)AGATA (starting with SuccessablesARR1.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for (A/G)AGATA, 1, AAGATA at 1525.
- positive strand, negative direction, looking for (A/G)AGATA, 3, AAGATA at 2177, AAGATA at 1595, AAGATA at 57.
- positive strand, positive direction, looking for (A/G)AGATA, 0.
- negative strand, positive direction, looking for (A/G)AGATA, 0.
- inverse complement, negative strand, negative direction, looking for TATCT(C/T), 4, TATCTT at 4080, TATCTC at 1711, TATCTT at 356, TATCTT at 101.
- inverse complement, positive strand, negative direction, looking for TATCT(C/T), 1, TATCTT at 1732.
- inverse complement, positive strand, positive direction, looking for TATCT(C/T), 1, TATCTT at 2628.
- inverse complement, negative strand, positive direction, looking for TATCT(C/T), 0.
ARR12 UTRs
Negative strand, negative direction: TATCTT at 4080.
ARR12 distal promoters
Negative strand, negative direction: TATCTC at 1711, AAGATA at 1525, TATCTT at 356, TATCTT at 101.
Positive strand, negative direction: AAGATA at 2177, TATCTT at 1732, AAGATA at 1595, AAGATA at 57.
Positive strand, positive direction: TATCTT at 2628.
Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.
See also
References
- ↑ 1.0 1.1 1.2 1.3 Mingtang Xie1, Hongyu Chen, Ling Huang, Ryan C. O’Neil1, Maxim N. Shokhirev & Joseph R. Ecker (23 April 2018). "A B-ARR-mediated cytokinin transcriptional network directs hormone cross-regulation and shoot development" (PDF). Nature Communications. 9 (1604): 1–13. doi:10.1038/s41467-018-03921-6. Retrieved 26 April 2021.