Gal4p gene transcriptions: Difference between revisions
mNo edit summary |
|||
| Line 10: | Line 10: | ||
"During the induction of galactose, the production of Gal4p can improve the PGAL1 activity 1000-fold [37]."<ref name=Tang/> | "During the induction of galactose, the production of Gal4p can improve the PGAL1 activity 1000-fold [37]."<ref name=Tang/> | ||
"We used the yeast Gal4p two-hybrid system (Fields and Sternglanz, 1994) to identify proteins that physically interact with GLD-1. We recovered two identical cDNAs in two-hybrid screens [...]. One (OG2.3) using GLD-1 residues 84-341 and the other (CD13.1) using residues 273-457, both fused to the Gal4p DNA binding domain [...]."<ref name=Clifford>{{ cite journal | |||
|author=Robert Clifford, Min-Ho Lee, Sudhir Nayak, Mitsue Ohmachi, Flav Giorgini and Tim Schedl | |||
|title=FOG-2, a novel F-box containing protein, associates with the GLD-1 RNA binding protein and directs male sex determination in the ''C. elegans'' hermaphrodite germline | |||
|journal=Development | |||
|date=December 2000 | |||
|volume=127 | |||
|issue=24 | |||
|pages=5265-76 | |||
|url=https://dev.biologists.org/content/develop/127/24/5265.full.pdf | |||
|arxiv= | |||
|bibcode= | |||
|doi= | |||
|pmid=11076749 | |||
|accessdate=10 August 2020 }}</ref> | |||
==Consensus sequences== | ==Consensus sequences== | ||
Revision as of 15:50, 19 September 2020
Associate Editor(s)-in-Chief: Henry A. Hoff
"PGAL1 is often used repeatedly for the expression of different enzymes in the construction of metabolic pathways [13], so the gene copy number has to be increased to compensate for the insufficient promoter strength [8], and the utilization of too many galactose-inducible promoters may interfere with the metabolism of galactose due to the depletion of the transcription activator Gal4p [14]."[1]
Galactose-inducible "promoters have various conserved UASGAL sites, a 17-bp consensus sequence 5′-CCGNNNNNNNNNNNCGG-3′ that is recognized by the transcription activator Gal4p to control their expression activities [38]."[1]
Human genes
Interactions
"During the induction of galactose, the production of Gal4p can improve the PGAL1 activity 1000-fold [37]."[1]
"We used the yeast Gal4p two-hybrid system (Fields and Sternglanz, 1994) to identify proteins that physically interact with GLD-1. We recovered two identical cDNAs in two-hybrid screens [...]. One (OG2.3) using GLD-1 residues 84-341 and the other (CD13.1) using residues 273-457, both fused to the Gal4p DNA binding domain [...]."[2]
Consensus sequences
Upstream activating sequence for Gal4p is 5'-CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG-3' [38].[1]
Samplings
Copying 5'-CGGACCGC-3' in "⌘F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.
See also
References
- ↑ 1.0 1.1 1.2 1.3 Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check
|pmid=value (help). Retrieved 18 September 2020. - ↑ Robert Clifford, Min-Ho Lee, Sudhir Nayak, Mitsue Ohmachi, Flav Giorgini and Tim Schedl (December 2000). "FOG-2, a novel F-box containing protein, associates with the GLD-1 RNA binding protein and directs male sex determination in the C. elegans hermaphrodite germline" (PDF). Development. 127 (24): 5265–76. PMID 11076749. Retrieved 10 August 2020.