Tec1p gene transcriptions

Associate Editor(s)-in-Chief: Henry A. Hoff

Human genes

Interactions

Consensus sequences

The upstream activating sequence (UAS) for Tec1p is 5'-GAATGT-3'.^[1]

Tec1 samplings

Copying GAATGT in "⌘F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence GAATGT (starting with SuccessablesTEC.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

negative strand, negative direction, looking for GAATGT, 0.
positive strand, negative direction, looking for GAATGT, 0.
positive strand, positive direction, looking for GAATGT, 0.
negative strand, positive direction, looking for GAATGT, 0.
complement, negative strand, negative direction, looking for CTTACA, 0.
complement, positive strand, negative direction, looking for CTTACA, 0.
complement, positive strand, positive direction, looking for CTTACA, 0.
complement, negative strand, positive direction, looking for CTTACA, 0.
inverse complement, negative strand, negative direction, looking for ACATTC, 0.
inverse complement, positive strand, negative direction, looking for ACATTC, 0.
inverse complement, positive strand, positive direction, looking for ACATTC, 1, ACATTC at 2456.
inverse complement, negative strand, positive direction, looking for ACATTC, 0.
inverse negative strand, negative direction, looking for TGTAAG, 0.
inverse positive strand, negative direction, looking for TGTAAG, 0.
inverse positive strand, positive direction, looking for TGTAAG, 0.
inverse negative strand, positive direction, looking for TGTAAG, 1, TGTAAG at 2456.

TEC positive direction (4050-1) distal promoters

Positive strand, positive direction: ACATTC at 2456.

TEC random dataset samplings

TECr0: 0.
TECr1: 0.
TECr2: 3, GAATGT at 4493, GAATGT at 1294, GAATGT at 918.
TECr3: 1, GAATGT at 4317.
TECr4: 1, GAATGT at 1101.
RDr5: 0.
RDr6: 0.
RDr7: 0.
RDr8: 0.
RDr9: 0.
RDr0ci: 0.
RDr1ci: 0.
RDr2ci: 0.
RDr3ci: 0.
RDr4ci: 0.
RDr5ci: 0.
RDr6ci: 0.
RDr7ci: 0.
RDr8ci: 0.
RDr9ci: 0.

TECr arbitrary (evens) (4560-2846) UTRs

TECr2: GAATGT at 4493.

TECr alternate (odds) (4560-2846) UTRs

TECr3: GAATGT at 4317.

RDr arbitrary negative direction (evens) (2846-2811) core promoters

RDr alternate negative direction (odds) (2846-2811) core promoters

TECr arbitrary positive direction (odds) (4445-4265) core promoters

TECr3: GAATGT at 4317.

RDr alternate positive direction (evens) (4445-4265) core promoters

RDr arbitrary negative direction (evens) (2811-2596) proximal promoters

RDr alternate negative direction (odds) (2811-2596) proximal promoters

RDr arbitrary positive direction (odds) (4265-4050) proximal promoters

RDr alternate positive direction (evens) (4265-4050) proximal promoters

TECr arbitrary negative direction (evens) (2596-1) distal promoters

TECr2: GAATGT at 1294, GAATGT at 918.
TECr4: GAATGT at 1101.

RDr alternate negative direction (odds) (2596-1) distal promoters

RDr arbitrary positive direction (odds) (4050-1) distal promoters

TECr alternate positive direction (evens) (4050-1) distal promoters

TECr2: GAATGT at 1294, GAATGT at 918.
TECr4: GAATGT at 1101.

TEC analysis and results

The upstream activating sequence (UAS) for Tec1p is GAATGT.^[1]

Reals or randoms	Promoters	direction	Numbers	Strands	Occurrences	Averages (± 0.1)
Reals	UTR	negative	0	2	0	0
Randoms	UTR	arbitrary negative	0	10	0	0
Randoms	UTR	alternate negative	0	10	0	0
Reals	Core	negative	0	2	0	0
Randoms	Core	arbitrary negative	0	10	0	0
Randoms	Core	alternate negative	0	10	0	0
Reals	Core	positive	0	2	0	0
Randoms	Core	arbitrary positive	0	10	0	0
Randoms	Core	alternate positive	0	10	0	0
Reals	Proximal	negative	0	2	0	0
Randoms	Proximal	arbitrary negative	0	10	0	0
Randoms	Proximal	alternate negative	0	10	0	0
Reals	Proximal	positive	0	2	0	0
Randoms	Proximal	arbitrary positive	0	10	0	0
Randoms	Proximal	alternate positive	0	10	0	0
Reals	Distal	negative	0	2	0	0
Randoms	Distal	arbitrary negative	0	10	0	0
Randoms	Distal	alternate negative	0	10	0	0
Reals	Distal	positive	1	2	0.5	0.5 ± 0.5 (-+0,++1)
Randoms	Distal	arbitrary positive	0	10	0	0
Randoms	Distal	alternate positive	0	10	0	0

Comparison:

The occurrences of real TECs are greater than the randoms. This suggests that the real TECs are likely active or activable.

References

↑ ^1.0 ^1.1 Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.

External links

[Tang-1] 1.0 ^1.1 Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.

[1]

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