H and ACA box gene transcriptions
Editor-In-Chief: Henry A. Hoff
Consensus sequences
"The box H/ACA snoRNAs were most recently recognized as a small RNA family by virtue of an ACA trinucleotide located 3 nt upstream of the mature snoRNA 3' end (41). In addition to this ACA box, they have the consensus H box sequence (5'-ANANNA-3') but have no other primary sequence identity. Despite this lack of primary sequence conservation, the H and ACA boxes are embedded in an evolutionarily conserved hairpin-hinge-hairpin-tail core secondary structure with the H box in the single-stranded hinge region and the ACA box in the single-stranded tail (5, 16)."[1]
The "3' end of mature hTR (45) has an ACA trinucleotide 3 nt upstream of its 3' end. In addition, the 3' region of hTR contains a single H box consensus sequence (5'-AGAGGA-3')."[1]
"Comparison with the murine telomerase RNA (mTR) (7) suggests that the snoRNA-like features of hTR are evolutionarily conserved. The mTR 3' end (nt 169 to 397 as numbered in reference 25) has ~76% sequence identity with the corresponding region of hTR (nt 211 to 451) and includes consensus H (5'-ACAGGA-3') and ACA box sequences."[1]
An H box has a consensus sequence of 3'-ACACCA-5'.[2]
The combined consensus sequence is 5'-ACAGGA-3'.[1]
H and ACA boxes in promoters of A1BG
For the Basic programs (starting with SuccessablesHACA.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction is SuccessablesHACA--.bas, looking for 5'-ACAGGA-3', 0.
- negative strand, positive direction is SuccessablesHACA-+.bas, looking for 5'-ACAGGA-3', 1, 5'-ACAGGA-3' at 3572.
- positive strand, negative direction is SuccessablesHACA+-.bas, looking for 5'-ACAGGA-3', 1, 5'-ACAGGA-3' at 2690.
- positive strand, positive direction is SuccessablesHACA++.bas, looking for 5'-ACAGGA-3', 1, 5'-ACAGGA-3' at 3620.
- complement, negative strand, negative direction is SuccessablesHACAc--.bas, looking for 5'-TGTCCT-3', 0.
- complement, negative strand, positive direction is SuccessablesHACAc-+.bas, looking for 5'-TGTCCT-3', 0.
- complement, positive strand, negative direction is SuccessablesHACAc+-.bas, looking for 5'-TGTCCT-3', 0.
- complement, positive strand, positive direction is SuccessablesHACAc++.bas, looking for 5'-TGTCCT-3', 0.
- inverse complement, negative strand, negative direction is SuccessablesHACAci--.bas, looking for 5'-TCCTGT-3', 0.
- inverse complement, negative strand, positive direction is SuccessablesHACAci-+.bas, looking for 5'-TCCTGT-3', 0.
- inverse complement, positive strand, negative direction is SuccessablesHACAci+-.bas, looking for 5'-TCCTGT-3', 0.
- inverse complement, positive strand, positive direction is SuccessablesHACAci++.bas, looking for 5'-TCCTGT-3', 0.
- inverse, negative strand, negative direction, is SuccessablesHACAi--.bas, looking for 5'-AGGACA-3', 0.
- inverse, negative strand, positive direction, is SuccessablesHACAi-+.bas, looking for 5'-AGGACA-3', 0.
- inverse, positive strand, negative direction, is SuccessablesHACAi+-.bas, looking for 5'-AGGACA-3', 0.
- inverse, positive strand, positive direction, is SuccessablesHACAi++.bas, looking for 5'-AGGACA-3', 0.
Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.
Initial content for this page in some instances came from Wikiversity.
See also
References
- ↑ 1.0 1.1 1.2 1.3 James R. Mitchell, Jeffrey Cheng, ang Kathleen Collins (January 1999). "A Box H/ACA Small Nucleolar RNA-Like Domain at the Human Telomerase RNA 3' End" (PDF). Molecular and Cellular Biology. 19 (1): 567–576. Retrieved 5 November 2018.
- ↑ Timofey S. Rozhdestvensky, Thean Hock Tang, Inna V. Tchirkova, Jürgen Brosius, Jean‐Pierre Bachellerie and Alexander Hüttenhofer (2003). "Binding of L7Ae protein to the K‐turn of archaeal snoRNAs: a shared RNA binding motif for C/D and H/ACA box snoRNAs in Archaea". Nucleic Acids Research. 31 (3): 869–77. doi:10.1093/nar/gkg175. Retrieved 2014-06-08.