Gal4p gene transcriptions

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Associate Editor(s)-in-Chief: Henry A. Hoff

PGAL1 is often used repeatedly for the expression of different enzymes in the construction of metabolic pathways [13], so the gene copy number has to be increased to compensate for the insufficient promoter strength [8], and the utilization of too many galactose-inducible promoters may interfere with the metabolism of galactose due to the depletion of the transcription activator Gal4p [14].

Galactose-inducible "promoters have various conserved UASGAL sites, a 17-bp consensus sequence 5′-CCGNNNNNNNNNNNCGG-3′ that is recognized by the transcription activator Gal4p to control their expression activities [38]."[1]

Human genes

Interactions

"During the induction of galactose, the production of Gal4p can improve the PGAL1 activity 1000-fold [37]."[1]

Consensus sequences

Upstream activating sequence for Gal4p is 5'-CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG-3' [38].[1]

Samplings

Copying 5'-CGGACCGC-3' in "⌘F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.

See also

References

  1. 1.0 1.1 1.2 Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.

External links