K-box gene transcriptions
Associate Editor(s)-in-Chief: Henry A. Hoff
"In fact, the groE genes were greatly induced upon heat shock in the hrcA mutant which was dark-treated prior to the heat shock in order to keep the background initial level of the groE mRNA accumulation as low as possible. These results led us to find a novel regulatory sequence which is involved in a heat and/or light mediated regulation of the groESL1 operon (Kojima and Nakamoto, 2007). This DNA element (GTTCGG-NNAN-CCNNAC) is located upstream of the groE promoters. It is highly conserved among the groESL1 operons from various cyanobacterial genomes. We designated this DNA element to be K-box. Removal of the K-box containing region upstream of the groESL1 promoter abolished heat- and/or light-induced transcription of the operon completely. K-box is also present upstream of dnaK2, one of the three dnaK genes in cyanobacteria (Kojima and Nakamoto, 2007; Sato et al., 2007)."[1]
K-box samplings
Copying a responsive elements consensus sequence GTTCGG-NNAN-CCNNAC and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence GTTCGG-NNAN-CCNNAC (starting with SuccessablesK-box.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for AAAAAAAA, 0.
- positive strand, negative direction, looking for AAAAAAAA, 0.
- positive strand, positive direction, looking for AAAAAAAA, 0.
- negative strand, positive direction, looking for AAAAAAAA, 0.
- complement, negative strand, negative direction, looking for TTTTTTTT, 0.
- complement, positive strand, negative direction, looking for TTTTTTTT, 0.
- complement, positive strand, positive direction, looking for TTTTTTTT, 0.
- complement, negative strand, positive direction, looking for TTTTTTTT, 0.
- inverse complement, negative strand, negative direction, looking for TTTTTTTT, 0.
- inverse complement, positive strand, negative direction, looking for TTTTTTTT, 0.
- inverse complement, positive strand, positive direction, looking for TTTTTTTT, 0.
- inverse complement, negative strand, positive direction, looking for TTTTTTTT, 0.
- inverse negative strand, negative direction, looking for AAAAAAAA, 0.
- inverse positive strand, negative direction, looking for AAAAAAAA, 0.
- inverse positive strand, positive direction, looking for AAAAAAAA, 0.
- inverse negative strand, positive direction, looking for AAAAAAAA, 0.
K-box UTRs
K-box core promoters
K-box proximal promoters
K-box distal promoters
Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.
See also
References
- ↑ Masakazu Saito, Satoru Watanabe, Kaori Nimura-Matsune, Hirofumi Yoshikawa1,
8 Hitoshi Nakamoto (10 April 2020). "Regulation of the groESL1 transcription by the HrcA repressor and a novel transcription factor Orf7.5 in the cyanobacterium Synechococcus elongatus PCC7942". The Journal of General and Applied Microbiology. 66 (2): 85–92. doi:10.2323/jgam.2020.02.001. Retrieved 17 March 2021. line feed character in
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