Cat8p gene transcriptions
Associate Editor(s)-in-Chief: Henry A. Hoff
Human genes
Interactions
Consensus sequences
The upstream activating sequence (UAS) for Cat8p is 5'-CGGNBNVMHGGA-3', where N = A, C, G, T, B = C, G, T, V = A, C, G, M = A, C, and H = A, C, T; i.e. 5'-CGG(A/C/G/T)(C/G/T)(A/C/G/T)(A/C/G)(A/C)(A/C/T)GGA-3'.[1]
Hypotheses
- A1BG has no Cat8ps in either promoter.
- A1BG is not transcribed by a Cat8p.
- Cat8p does not participate in the transcription of A1BG.
Cat8p samplings
Copying 5'-CGGTCCGC-3' in "⌘F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence 5'-CGG(A/C/G/T)(C/G/T)(A/C/G/T)(A/C/G)(A/C)(A/C/T)GGA-3' (starting with SuccessablesCat8p.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for 5'-CGG(A/C/G/T)(C/G/T)(A/C/G/T)(A/C/G)(A/C)(A/C/T)GGA-3', 0.
- negative strand, positive direction, looking for 5'-CGG(A/C/G/T)(C/G/T)(A/C/G/T)(A/C/G)(A/C)(A/C/T)GGA-3', 0.
- positive strand, negative direction, looking for 5'-CGG(A/C/G/T)(C/G/T)(A/C/G/T)(A/C/G)(A/C)(A/C/T)GGA-3', 0.
- positive strand, positive direction, looking for 5'-CGG(A/C/G/T)(C/G/T)(A/C/G/T)(A/C/G)(A/C)(A/C/T)GGA-3', 0.
- complement, negative strand, negative direction, looking for 5'-GCC(A/C/G/T)(A/C/G)(A/C/G/T)(C/G/T)(G/T)(A/G/T)CCT-3', 0.
- complement, negative strand, positive direction, looking for 5'-GCC(A/C/G/T)(A/C/G)(A/C/G/T)(C/G/T)(G/T)(A/G/T)CCT-3', 0.
- complement, positive strand, negative direction, looking for 5'-GCC(A/C/G/T)(A/C/G)(A/C/G/T)(C/G/T)(G/T)(A/G/T)CCT-3', 0.
- complement, positive strand, positive direction, looking for 5'-GCC(A/C/G/T)(A/C/G)(A/C/G/T)(C/G/T)(G/T)(A/G/T)CCT-3', 0.
- inverse complement, negative strand, negative direction, looking for 5'-TCC(A/G/T)(G/T)(C/G/T)(A/C/G/T)(A/C/G)(A/C/G/T)CCG-3', 2, 5'-TCCGTGCCACCG-3' at 2528, 5'-TCCGTGCCACCG-3' at 657, and complements.
- inverse complement, negative strand, positive direction, looking for 5'-TCC(A/G/T)(G/T)(C/G/T)(A/C/G/T)(A/C/G)(A/C/G/T)CCG-3', 0.
- inverse complement, positive strand, negative direction, looking for 5'-TCC(A/G/T)(G/T)(C/G/T)(A/C/G/T)(A/C/G)(A/C/G/T)CCG-3', 0.
- inverse complement, positive strand, positive direction, looking for 5'-TCC(A/G/T)(G/T)(C/G/T)(A/C/G/T)(A/C/G)(A/C/G/T)CCG-3', 0.
- inverse negative strand, negative direction, looking for 5'-AGG(A/C/T)(A/C)(A/C/G)(A/C/G/T)(C/G/T)(A/C/G/T)GGC-3', 0.
- inverse negative strand, positive direction, looking for 5'-AGG(A/C/T)(A/C)(A/C/G)(A/C/G/T)(C/G/T)(A/C/G/T)GGC-3', 0.
- inverse positive strand, negative direction, looking for 5'-AGG(A/C/T)(A/C)(A/C/G)(A/C/G/T)(C/G/T)(A/C/G/T)GGC-3', 2, 5'-AGGCACGGTGGC-3' at 2528, 5'-AGGCACGGTGGC-3' at 657.
- inverse positive strand, positive direction, looking for 5'-AGG(A/C/T)(A/C)(A/C/G)(A/C/G/T)(C/G/T)(A/C/G/T)GGC-3', 0.
Cat8p distal promoters
Negative strand, negative direction: TCCGTGCCACCG at 2528, TCCGTGCCACCG at 657.
Cat8 random dataset samplings
- Cat8r0: 0.
- Cat8r1: 1, CGGGTTGATGGA at 1079.
- Cat8r2: 0.
- Cat8r3: 0.
- Cat8r4: 0.
- Cat8r5: 1, CGGCTCAACGGA at 575.
- Cat8r6: 1, CGGGCCAATGGA at 743.
- Cat8r7: 0.
- Cat8r8: 0.
- Cat8r9: 0.
- Cat8r0ci: 0.
- Cat8r1ci: 0.
- Cat8r2ci: 1, TCCTGTACACCG at 1210.
- Cat8r3ci: 0.
- Cat8r4ci: 1, TCCTGGGCGCCG at 2629.
- Cat8r5ci: 0.
- Cat8r6ci: 1, TCCAGTTGTCCG at 2642.
- Cat8r7ci: 1, TCCATCCGGCCG at 1909.
- Cat8r8ci: 1, TCCTGTTGCCCG at 3455.
- Cat8r9ci: 0.
Cat8r UTRs
- Cat8r8ci: TCCTGTTGCCCG at 3455.
Cat8r proximal promoters
- Cat8r4ci: TCCTGGGCGCCG at 2629.
- Cat8r6ci: TCCAGTTGTCCG at 2642.
Cat8r distal promoters
- Cat8r6: CGGGCCAATGGA at 743.
- Cat8r2ci: TCCTGTACACCG at 1210.
- Cat8r1: CGGGTTGATGGA at 1079.
- Cat8r5: CGGCTCAACGGA at 575.
- Cat8r7ci: TCCATCCGGCCG at 1909.
Cat8 analysis and results
The upstream activating sequence (UAS) for Cat8p is 5'-CGGNBNVMHGGA-3', where N = A, C, G, T, B = C, G, T, V = A, C, G, M = A, C, and H = A, C, T; i.e. 5'-CGG(A/C/G/T)(C/G/T)(A/C/G/T)(A/C/G)(A/C)(A/C/T)GGA-3'.[1]
Reals or randoms | Promoters | direction | Numbers | Strands | Occurrences | Averages (± 0.1) |
---|---|---|---|---|---|---|
Reals | UTR | negative | 0 | 2 | 0 | 0 |
Randoms | UTR | arbitrary negative | 0 | 10 | 0 | 0 |
Randoms | UTR | alternate negative | 0 | 10 | 0 | 0 |
Reals | Core | negative | 0 | 2 | 0 | 0 |
Randoms | Core | arbitrary negative | 0 | 10 | 0 | 0 |
Randoms | Core | alternate negative | 0 | 10 | 0 | 0 |
Reals | Core | positive | 0 | 2 | 0 | 0 |
Randoms | Core | arbitrary positive | 0 | 10 | 0 | 0 |
Randoms | Core | alternate positive | 0 | 10 | 0 | 0 |
Reals | Proximal | negative | 0 | 2 | 0 | 0 |
Randoms | Proximal | arbitrary negative | 0 | 10 | 0 | 0 |
Randoms | Proximal | alternate negative | 0 | 10 | 0 | 0 |
Reals | Proximal | positive | 0 | 2 | 0 | 0 |
Randoms | Proximal | arbitrary positive | 0 | 10 | 0 | 0 |
Randoms | Proximal | alternate positive | 0 | 10 | 0 | 0 |
Reals | Distal | negative | 0 | 2 | 0 | 0 |
Randoms | Distal | arbitrary negative | 0 | 10 | 0 | 0 |
Randoms | Distal | alternate negative | 0 | 10 | 0 | 0 |
Reals | Distal | positive | 0 | 2 | 0 | 0 |
Randoms | Distal | arbitrary positive | 0 | 10 | 0 | 0 |
Randoms | Distal | alternate positive | 0 | 10 | 0 | 0 |
Comparison:
The occurrences of real responsive element consensus sequences are greater than the randoms. This suggests that the real responsive element consensus sequences are likely active or activable.
Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.
See also
References
- ↑ 1.0 1.1 Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check
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value (help). Retrieved 18 September 2020.