CCCTC-binding factor gene transcriptions
Associate Editor(s)-in-Chief: Henry A. Hoff
Consensus sequences
"Experiments using chromatin immunoprecipitation exonuclease (ChIP-exo) uncovered a broad CTCF-binding motif that contains a 12–15 bp consensus sequence, 5′-NCA-NNA-G(G/A)N-GGC-(G/A)(C/G)(T/C)-3′ (Nakahashi et al., 2013, Rhee and Pugh, 2011) [...]."[1]
Hashimoto samplings
Copying the consensus of the CTCF: 5'-CACCAGG-3' and putting the sequence in "⌘F" finds no locations between ZSCAN22 and A1BG and CTCF: 5'-CACCAGGAGG-3' finds no locations between ZNF497 and A1BG as can be found by the computer programs.
For the Basic programs SuccessablesCFbox.bas written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for 5'-NCA-NNA-G(A/G)N-GGC-(A/G)(C/G)(C/T)-3'[1], 0.
- negative strand, positive direction, looking for 5'-NCA-NNA-G(A/G)N-GGC-(A/G)(C/G)(C/T)-3', 0.
- positive strand, negative direction, looking for 5'-NCA-NNA-G(A/G)N-GGC-(A/G)(C/G)(C/T)-3', 0.
- positive strand, positive direction, looking for 5'-NCA-NNA-G(A/G)N-GGC-(A/G)(C/G)(C/T)-3', 0.
- complement, negative strand, negative direction, looking for 5'-NGT-NNT-C(C/T)N-CCG-(C/T)(C/G)(A/G)-3', 0.
- complement, negative strand, positive direction, looking for 5'-NGT-NNT-C(C/T)N-CCG-(C/T)(C/G)(A/G)-3', 0.
- complement, positive strand, negative direction, looking for 5'-NGT-NNT-C(C/T)N-CCG-(C/T)(C/G)(A/G)-3', 0.
- complement, positive strand, positive direction, looking for 5'-NGT-NNT-C(C/T)N-CCG-(C/T)(C/G)(A/G)-3', 0.
- inverse complement, negative strand, negative direction, looking for 5'-(A/G)(C/G)(C/T)-GCC-N(C/T)C-TNN-TGN-3', 0.
- inverse complement, negative strand, positive direction, looking for 5'-(A/G)(C/G)(C/T)-GCC-N(C/T)C-TNN-TGN-3', 0.
- inverse complement, positive strand, negative direction, looking for 5'-(A/G)(C/G)(C/T)-GCC-N(C/T)C-TNN-TGN-3', 0.
- inverse complement, positive strand, positive direction, looking for 5'-(A/G)(C/G)(C/T)-GCC-N(C/T)C-TNN-TGN-3', 0.
- inverse, negative strand, negative direction, looking for 5'-(C/T)(C/G)(A/G)-CGG-N(A/G)G-ANN-ACN-3', 0.
- inverse, negative strand, positive direction, looking for 5'-(C/T)(C/G)(A/G)-CGG-N(A/G)G-ANN-ACN-3', 0.
- inverse, positive strand, negative direction, looking for 5'-(C/T)(C/G)(A/G)-CGG-N(A/G)G-ANN-ACN-3', 0.
- inverse, positive strand, positive direction, looking for 5'-(C/T)(C/G)(A/G)-CGG-N(A/G)G-ANN-ACN-3', 0.
Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.
See also
References
- ↑ 1.0 1.1 Hideharu Hashimoto, Dongxue Wang, John R. Horton, Xing Zhang, Victor G. Corces and Xiaodong Cheng (1 June 2017). "Structural Basis for the Versatile and Methylation-Dependent Binding of CTCF to DNA". Molecular Cell. 66 (5): 711–720.e3. doi:10.1016/j.molcel.2017.05.004. PMID 28529057. Retrieved 28 August 2020.