Replication initiator 1 is a protein that in humans is encoded by the REPIN1 gene .[1] [2]
Model organisms Model organisms have been used in the study of REPIN1 function. A conditional knockout mouse line called Repin1tm1a(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute .[3] phenotypic screen [4] [5] [6] [7] [8] [9]
Repin1 knockout mouse phenotype
Characteristic
Phenotype
All data available at.[4] [9]
Peripheral blood leukocytes 6 Weeks
Normal
Insulin
Normal
Haematology Normal
Homozygous viability at P14
Normal
Homozygous Fertility
Normal
Body weight
Normal
Neurological assessment
Normal
Grip strength
Normal
Dysmorphology Normal
Indirect calorimetry Abnormal
Glucose tolerance test Normal
Auditory brainstem response Normal
DEXA Normal
Radiography Normal
Eye morphology
Normal
Clinical chemistry Normal
Haematology Normal
Peripheral blood leukocytes 16 Weeks
Normal
Heart weight
Normal
Salmonella Normal
Cytotoxic T Cell Function
Normal
Spleen Immunophenotyping
Normal
Mesenteric Lymph Node Immunophenotyping
Normal
Bone Marrow Immunophenotyping
Normal
Epidermal Immune Composition
Normal
References
↑ Houchens CR, Montigny W, Zeltser L, Dailey L, Gilbert JM, Heintz NH (Jan 2000). "The dhfr oribeta-binding protein RIP60 contains 15 zinc fingers: DNA binding and looping by the central three fingers and an associated proline-rich region" . Nucleic Acids Research . 28 (2): 570–81. doi :10.1093/nar/28.2.570 . PMC 102514 PMID 10606657 . ↑ "Entrez Gene: REPIN1 replication initiator 1" .↑ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica . 88 : 925–7. doi :10.1111/j.1755-3768.2010.4142.x . ↑ 4.0 4.1 "International Mouse Phenotyping Consortium" .↑ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function" . Nature . 474 (7351): 337–42. doi :10.1038/nature10163 . PMC 3572410 PMID 21677750 . ↑ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature . 474 (7351): 262–3. doi :10.1038/474262a . PMID 21677718 . ↑ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell . 128 (1): 9–13. doi :10.1016/j.cell.2006.12.018 . PMID 17218247 . ↑ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes" . Cell . 154 (2): 452–64. doi :10.1016/j.cell.2013.06.022 . PMC 3717207 PMID 23870131 . ↑ 9.0 9.1 "Infection and Immunity Immunophenotyping (3i) Consortium" .
Further reading
Dailey L, Caddle MS, Heintz N, Heintz NH (Dec 1990). "Purification of RIP60 and RIP100, mammalian proteins with origin-specific DNA-binding and ATP-dependent DNA helicase activities" . Molecular and Cellular Biology . 10 (12): 6225–35. doi :10.1128/mcb.10.12.6225 . PMC 362897 PMID 2174103 . Caddle MS, Dailey L, Heintz NH (Dec 1990). "RIP60, a mammalian origin-binding protein, enhances DNA bending near the dihydrofolate reductase origin of replication" . Molecular and Cellular Biology . 10 (12): 6236–43. doi :10.1128/mcb.10.12.6236 . PMC 362898 PMID 2247056 . Mastrangelo IA, Held PG, Dailey L, Wall JS, Hough PV, Heintz N, Heintz NH (Aug 1993). "RIP60 dimers and multiples of dimers assemble link structures at an origin of bidirectional replication in the dihydrofolate reductase amplicon of Chinese hamster ovary cells". Journal of Molecular Biology . 232 (3): 766–78. doi :10.1006/jmbi.1993.1430 . PMID 8355269 . Montigny WJ, Houchens CR, Illenye S, Gilbert J, Coonrod E, Chang YC, Heintz NH (May 2001). "Condensation by DNA looping facilitates transfer of large DNA molecules into mammalian cells" . Nucleic Acids Research . 29 (9): 1982–8. doi :10.1093/nar/29.9.1982 . PMC 37261 PMID 11328883 . Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, Li Y, Xu C, Fang R, Guegler K, Rao MS, Mandalam R, Lebkowski J, Stanton LW (Jun 2004). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nature Biotechnology . 22 (6): 707–16. doi :10.1038/nbt971 . PMID 15146197 . Kim MY, Jeong BC, Lee JH, Kee HJ, Kook H, Kim NS, Kim YH, Kim JK, Ahn KY, Kim KK (Aug 2006). "A repressor complex, AP4 transcription factor and geminin, negatively regulates expression of target genes in nonneuronal cells" . Proceedings of the National Academy of Sciences of the United States of America . 103 (35): 13074–9. doi :10.1073/pnas.0601915103 . PMC 1551900 PMID 16924111 .
External links This article incorporates text from the United States National Library of Medicine , which is in the public domain .
(2) Zinc finger DNA-binding domains
(2.1) Nuclear receptor (Cys4 )
subfamily 1 subfamily 2 subfamily 3 subfamily 4 subfamily 5 subfamily 6 subfamily 0
(2.2) Other Cys4 (2.3) Cys2 His2 (2.4) Cys6 (2.5) Alternating composition (2.6) WRKY
(4) β-Scaffold factors with minor groove contacts
(0) Other transcription factors
