User:Marshallsumter: Difference between revisions
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==Human genes== | ==Human genes== | ||
==Gene expressions== | ==Gene expressions== | ||
Line 11: | Line 11: | ||
==Interactions== | ==Interactions== | ||
==Consensus sequences== | ==Consensus sequences== | ||
==Binding site for== | ==Binding site for== | ||
==Complement copies== | |||
{{main|Complement copy gene transcriptions}} | |||
==Inverse copies== | ==Inverse copies== | ||
{{main|Inverse copy gene transcriptions}} | |||
==Complement-inverse copies== | |||
==Enhancer activity== | ==Enhancer activity== | ||
==Promoter occurrences== | ==Promoter occurrences== | ||
==Hypotheses== | ==Hypotheses== | ||
{{main|Hypotheses}} | {{main|Hypotheses}} | ||
# A1BG has no | # A1BG has no regulatory elements in either promoter. | ||
# A1BG is not transcribed by | # A1BG is not transcribed by a regulatory element. | ||
# | # No regulatory element participates in the transcription of A1BG. | ||
==Response element samplings== | |||
Copying a responsive elements consensus sequence AAAAAAAA and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs. | |||
For the Basic programs testing consensus sequence AAAAAAAA (starting with SuccessablesAAA.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found: | |||
# negative strand, negative direction, looking for AAAAAAAA, 0. | |||
# positive strand, negative direction, looking for AAAAAAAA, 0. | |||
# negative strand, positive direction, looking for AAAAAAAA, 0. | |||
# positive strand, positive direction, looking for AAAAAAAA, 0. | |||
# inverse complement, negative strand, negative direction, looking for TTTTTTTT, 0. | |||
# inverse complement, positive strand, negative direction, looking for TTTTTTTT, 0. | |||
# inverse complement, negative strand, positive direction, looking for TTTTTTTT, 0. | |||
# inverse complement, positive strand, positive direction, looking for TTTTTTTT, 0. | |||
===AAA (4560-2846) UTRs=== | |||
===AAA negative direction (2846-2811) core promoters=== | |||
===AAA positive direction (4445-4265) core promoters=== | |||
===AAA negative direction (2811-2596) proximal promoters=== | |||
===AAA positive direction (4265-4050) proximal promoters=== | |||
===AAA negative direction (2596-1) distal promoters=== | |||
===AAA positive direction (4050-1) distal promoters=== | |||
==Response element random dataset samplings== | |||
# RDr0: 0. | |||
# RDr1: 0. | |||
# RDr2: 0. | |||
# RDr3: 0. | |||
# RDr4: 0. | |||
# RDr5: 0. | |||
# RDr6: 0. | |||
# RDr7: 0. | |||
# RDr8: 0. | |||
# RDr9: 0. | |||
# RDr0ci: 0. | |||
# RDr1ci: 0. | |||
# RDr2ci: 0. | |||
# RDr3ci: 0. | |||
# RDr4ci: 0. | |||
# RDr5ci: 0. | |||
# RDr6ci: 0. | |||
# RDr7ci: 0. | |||
# RDr8ci: 0. | |||
# RDr9ci: 0. | |||
===RDr arbitrary (evens) (4560-2846) UTRs=== | |||
===RDr alternate (odds) (4560-2846) UTRs=== | |||
===RDr arbitrary negative direction (evens) (2846-2811) core promoters=== | |||
== | ===RDr alternate negative direction (odds) (2846-2811) core promoters=== | ||
===RDr arbitrary positive direction (odds) (4445-4265) core promoters=== | |||
===RDr alternate positive direction (evens) (4445-4265) core promoters=== | |||
===RDr arbitrary negative direction (evens) (2811-2596) proximal promoters=== | |||
===RDr alternate negative direction (odds) (2811-2596) proximal promoters=== | |||
===RDr arbitrary positive direction (odds) (4265-4050) proximal promoters=== | |||
# | |||
===RDr alternate positive direction (evens) (4265-4050) proximal promoters=== | |||
===RDr arbitrary negative direction (evens) (2596-1) distal promoters=== | |||
===RDr alternate negative direction (odds) (2596-1) distal promoters=== | |||
===RDr arbitrary positive direction (odds) (4050-1) distal promoters=== | |||
===RDr alternate positive direction (evens) (4050-1) distal promoters=== | |||
==Response element analysis and results== | |||
{{main|Complex locus A1BG and ZNF497#Name of response elements}} | |||
The Pax-4 homeodomain [HD] was shown to preferentially dimerize on DNA sequences consisting of an inverted TAAT motif, separated by 4-nucleotide spacing.<ref name=Zhang>{{ cite journal | |||
|author=Bosen Zhang, Liwei Song, Jiali Cai, Lei Li, Hong Xu, Mengying Li, Jiamin Wang, Minmin Shi, Hao Chen, Hao Jia, and Zhaoyuan Hou | |||
|title=The LIM protein Ajuba/SP1 complex forms a feed forward loop to induce SP1 target genes and promote pancreatic cancer cell proliferation | |||
|journal=Journal of Experimental and Clinical Cancer Research | |||
|date=17 May 2019 | |||
|volume=38 | |||
|issue= | |||
|pages=205 | |||
|url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525466/ | |||
|arxiv= | |||
|bibcode= | |||
|doi=10.1186/s13046-019-1203-2 | |||
|pmid=31101117 | |||
|accessdate=27 February 2021 }}</ref> | |||
= | {|class="wikitable" | ||
|- | |||
! Reals or randoms !! Promoters !! direction !! Numbers !! Strands !! Occurrences !! Averages (± 0.1) | |||
|- | |||
| Reals || UTR || negative || 0 || 2 || 0 || 0 | |||
|- | |||
| Randoms || UTR || arbitrary negative || 0 || 10 || 0 || 0 | |||
|- | |||
| Randoms || UTR || alternate negative || 0 || 10 || 0 || 0 | |||
|- | |||
| Reals || Core || negative || 0 || 2 || 0 || 0 | |||
|- | |||
| Randoms || Core || arbitrary negative || 0 || 10 || 0 || 0 | |||
|- | |||
| Randoms || Core || alternate negative || 0 || 10 || 0 || 0 | |||
|- | |||
| Reals || Core || positive || 0 || 2 || 0 || 0 | |||
|- | |||
| Randoms || Core || arbitrary positive || 0 || 10 || 0 || 0 | |||
|- | |||
| Randoms || Core || alternate positive || 0 || 10 || 0 || 0 | |||
|- | |||
| Reals || Proximal || negative || 0 || 2 || 0 || 0 | |||
|- | |||
| Randoms || Proximal || arbitrary negative || 0 || 10 || 0 || 0 | |||
|- | |||
| Randoms || Proximal || alternate negative || 0 || 10 || 0 || 0 | |||
|- | |||
| Reals || Proximal || positive || 0 || 2 || 0 || 0 | |||
|- | |||
| Randoms || Proximal || arbitrary positive || 0 || 10 || 0 || 0 | |||
|- | |||
| Randoms || Proximal || alternate positive || 0 || 10 || 0 || 0 | |||
|- | |||
| Reals || Distal || negative || 0 || 2 || 0 || 0 | |||
|- | |||
| Randoms || Distal || arbitrary negative || 0 || 10 || 0 || 0 | |||
|- | |||
| Randoms || Distal || alternate negative || 0 || 10 || 0 || 0 | |||
|- | |||
| Reals || Distal || positive || 0 || 2 || 0 || 0 | |||
|- | |||
| Randoms || Distal || arbitrary positive || 0 || 10 || 0 || 0 | |||
|- | |||
| Randoms || Distal || alternate positive || 0 || 10 || 0 || 0 | |||
|} | |||
Comparison: | |||
The occurrences of real responsive element consensus sequences are greater than the randoms. This suggests that the real responsive element consensus sequences are likely active or activable. | |||
==Acknowledgements== | ==Acknowledgements== | ||
Line 89: | Line 198: | ||
==See also== | ==See also== | ||
{{div col|colwidth=20em}} | {{div col|colwidth=20em}} | ||
* [[A1BG gene transcription core promoters]] | |||
* [[A1BG gene transcriptions]] | * [[A1BG gene transcriptions]] | ||
* [[A1BG regulatory elements and regions]] | |||
* [[A1BG response element gene transcriptions]] | |||
* [[A1BG response element negative results]] | |||
* [[A1BG response element positive results]] | |||
* [[Complex locus A1BG and ZNF497]] | * [[Complex locus A1BG and ZNF497]] | ||
{{Div col end}} | {{Div col end}} | ||
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<!-- footer templates --> | <!-- footer templates --> | ||
{{Gene project}} | {{Gene project}}{{tlx|Phosphate biochemistry}}{{Transcription factors and intracellular receptors}} | ||
<!-- footer categories --> | <!-- footer categories --> | ||
This user is also an author, contributor, and editor at | This user is also an author, contributor, and editor at |
Latest revision as of 05:24, 29 January 2023
Editor-In-Chief: Henry A. Hoff
On recent contributions: Associate Editor(s)-in-Chief: Henry A. Hoff
Human genes
Gene expressions
Interactions
Consensus sequences
Binding site for
Complement copies
Inverse copies
Complement-inverse copies
Enhancer activity
Promoter occurrences
Hypotheses
- A1BG has no regulatory elements in either promoter.
- A1BG is not transcribed by a regulatory element.
- No regulatory element participates in the transcription of A1BG.
Response element samplings
Copying a responsive elements consensus sequence AAAAAAAA and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence AAAAAAAA (starting with SuccessablesAAA.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for AAAAAAAA, 0.
- positive strand, negative direction, looking for AAAAAAAA, 0.
- negative strand, positive direction, looking for AAAAAAAA, 0.
- positive strand, positive direction, looking for AAAAAAAA, 0.
- inverse complement, negative strand, negative direction, looking for TTTTTTTT, 0.
- inverse complement, positive strand, negative direction, looking for TTTTTTTT, 0.
- inverse complement, negative strand, positive direction, looking for TTTTTTTT, 0.
- inverse complement, positive strand, positive direction, looking for TTTTTTTT, 0.
AAA (4560-2846) UTRs
AAA negative direction (2846-2811) core promoters
AAA positive direction (4445-4265) core promoters
AAA negative direction (2811-2596) proximal promoters
AAA positive direction (4265-4050) proximal promoters
AAA negative direction (2596-1) distal promoters
AAA positive direction (4050-1) distal promoters
Response element random dataset samplings
- RDr0: 0.
- RDr1: 0.
- RDr2: 0.
- RDr3: 0.
- RDr4: 0.
- RDr5: 0.
- RDr6: 0.
- RDr7: 0.
- RDr8: 0.
- RDr9: 0.
- RDr0ci: 0.
- RDr1ci: 0.
- RDr2ci: 0.
- RDr3ci: 0.
- RDr4ci: 0.
- RDr5ci: 0.
- RDr6ci: 0.
- RDr7ci: 0.
- RDr8ci: 0.
- RDr9ci: 0.
RDr arbitrary (evens) (4560-2846) UTRs
RDr alternate (odds) (4560-2846) UTRs
RDr arbitrary negative direction (evens) (2846-2811) core promoters
RDr alternate negative direction (odds) (2846-2811) core promoters
RDr arbitrary positive direction (odds) (4445-4265) core promoters
RDr alternate positive direction (evens) (4445-4265) core promoters
RDr arbitrary negative direction (evens) (2811-2596) proximal promoters
RDr alternate negative direction (odds) (2811-2596) proximal promoters
RDr arbitrary positive direction (odds) (4265-4050) proximal promoters
RDr alternate positive direction (evens) (4265-4050) proximal promoters
RDr arbitrary negative direction (evens) (2596-1) distal promoters
RDr alternate negative direction (odds) (2596-1) distal promoters
RDr arbitrary positive direction (odds) (4050-1) distal promoters
RDr alternate positive direction (evens) (4050-1) distal promoters
Response element analysis and results
The Pax-4 homeodomain [HD] was shown to preferentially dimerize on DNA sequences consisting of an inverted TAAT motif, separated by 4-nucleotide spacing.[1]
Reals or randoms | Promoters | direction | Numbers | Strands | Occurrences | Averages (± 0.1) |
---|---|---|---|---|---|---|
Reals | UTR | negative | 0 | 2 | 0 | 0 |
Randoms | UTR | arbitrary negative | 0 | 10 | 0 | 0 |
Randoms | UTR | alternate negative | 0 | 10 | 0 | 0 |
Reals | Core | negative | 0 | 2 | 0 | 0 |
Randoms | Core | arbitrary negative | 0 | 10 | 0 | 0 |
Randoms | Core | alternate negative | 0 | 10 | 0 | 0 |
Reals | Core | positive | 0 | 2 | 0 | 0 |
Randoms | Core | arbitrary positive | 0 | 10 | 0 | 0 |
Randoms | Core | alternate positive | 0 | 10 | 0 | 0 |
Reals | Proximal | negative | 0 | 2 | 0 | 0 |
Randoms | Proximal | arbitrary negative | 0 | 10 | 0 | 0 |
Randoms | Proximal | alternate negative | 0 | 10 | 0 | 0 |
Reals | Proximal | positive | 0 | 2 | 0 | 0 |
Randoms | Proximal | arbitrary positive | 0 | 10 | 0 | 0 |
Randoms | Proximal | alternate positive | 0 | 10 | 0 | 0 |
Reals | Distal | negative | 0 | 2 | 0 | 0 |
Randoms | Distal | arbitrary negative | 0 | 10 | 0 | 0 |
Randoms | Distal | alternate negative | 0 | 10 | 0 | 0 |
Reals | Distal | positive | 0 | 2 | 0 | 0 |
Randoms | Distal | arbitrary positive | 0 | 10 | 0 | 0 |
Randoms | Distal | alternate positive | 0 | 10 | 0 | 0 |
Comparison:
The occurrences of real responsive element consensus sequences are greater than the randoms. This suggests that the real responsive element consensus sequences are likely active or activable.
Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.
Initial content for this page in some instances came from Wikiversity.
Initial content for this page in some instances came from Wikipedia.
Initial content for this page in some instances incorporates text from the United States National Library of Medicine.
See also
References
- ↑ Bosen Zhang, Liwei Song, Jiali Cai, Lei Li, Hong Xu, Mengying Li, Jiamin Wang, Minmin Shi, Hao Chen, Hao Jia, and Zhaoyuan Hou (17 May 2019). "The LIM protein Ajuba/SP1 complex forms a feed forward loop to induce SP1 target genes and promote pancreatic cancer cell proliferation". Journal of Experimental and Clinical Cancer Research. 38: 205. doi:10.1186/s13046-019-1203-2. PMID 31101117. Retrieved 27 February 2021.
External links
- GenomeNet KEGG database
- Home - Gene - NCBI
- NCBI All Databases Search
- NCBI Site Search
- PubChem Public Chemical Database
This user is also an author, contributor, and editor at